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Line 24:		Line 24:
	32.5ul ddH<sub>2</sub>O		32.5ul ddH<sub>2</sub>O
-	|94°C	+	|94°C 5'
-	94°C	+
-	59°C	+	94°C   30''
-	68°C	+
-	68°C	+	59°C   30''
-	4°C <math>\infty</math>	+
		+	68°C   35''
		+
		+	68°C   1.5'
		+
		+	4°C forever
	|-		|-

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Revision as of 15:00, 10 October 2007

Protocol for PCR reactions A~G

For amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.

PCR mix	PCR program
5ul 10x buffer\\ 5ul Enhancer 0.6ul dNTPs (25mM stock) 2.5ul MgSO4 (Supplied in PCR kit) 1.5ul Primer I (10uM) 1.5ul Primer II (10uM) 1ul Template (pJS010) 0.4ul Pfx Platinum (Invitrogen) 32.5ul ddH2O	94°C 5' 94°C 30 59°C 30 68°C 35 68°C 1.5' 4°C forever
50ul Total

|-

PCR mix

|5ul 10x buffer\\

5ul Enhancer

0.6ul dNTPs (25mM stock)

2.5ul MgSO4 (Supplied in PCR kit)

1.5ul Primer I (10uM)

1.5ul Primer II (10uM)

1ul Template (pJS010)

0.4ul Pfx Platinum (Invitrogen)

32.5ul ddH₂O

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50ul Total | |-