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	::-I763032+pSB4A3;		::-I763032+pSB4A3;
	::-I763007+pSB4A3.		::-I763007+pSB4A3.
		+	*We inoculate:
		+	::-

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Revision as of 08:08, 11 October 2007

10/08/07

• We inoculate:

-I763031 (low copy plasmid);

-Plac-CI-Lacy (low copy plasmid);

-E.Coli;

-I763032 (high copy plasmid).

• Digestion of:

-I763035 with Eco/Pst1;

-I763007 with Eco/spe;

-Ptet-LacI-T with Eco/Xba;

-pSB4A3 with Spe/Xba.

• Ligation for:

-I763035+pSB4A3;

-I763007+Ptet-lacI-t;

-I763007+pSB4A3;

-I763032+pSB4A3.

• Run on electrophoresis gel.
• I763007 and Ptet-lacI-T band extraction.
• O/N ligation for:

-Ptet-LacI-T+I763007;

-Ptet-LacI+pSB4A3;

-I763032+pSB4A3;

-I763007+pSB4A3.

• We inoculate:

-

10/09/07

10/10/07

10/11/07

10/12/07

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