Berkeley UC

From 2007.igem.org

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'''The necessity''' of cheap, available, disease free, and universally usable blood substitutes is undisputed. There are currently no blood substitutes approved for use in the US or the UK, and whole blood is almost always in short supply. Developing countries have the greatest need for blood transfusions, however many lack the necessary donation and storage infrastructure and the required number of healthy donors.  To address this problem, we are developing an innovative and inexpensive blood substitute constructed from ''E. coli'' bacteria engineered to include the critical capabilities of human erythrocytes.  Our bacterial system includes the ability to safely exist in the bloodstream, carry oxygen with hemoglobin, and be stored for prolonged periods in a freeze-dried state.<br><br>
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''' ''Support for Berkeley iGEM 2007 was generously provided by SynBERC and The Camille and Henry Dreyfus Foundation, Inc.'' '''
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<h3>Project Modules</h3>
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|-
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<div style="position:relative;"><div style="position:absolute; top:-1px;">[[Image:Berk-Icon-Oxygen.png]]</div>[[Image:Berk-Pixel-Wide-Aligner.gif|121px]]</div>
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<b>[[BerkiGEM2007Present1 | Oxygen Carrying]]</b><p><i>Our system is designed to produce Hemoglobin, Heme, and the necessary chaperones and detoxifying agents to promote the transport of oxygen throughout the bloodstream.&nbsp; We also investigated alternates to hemoglobin and other strategies for its production.</i></p>
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|-
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|valign="top" style="padding-bottom: 3px; padding-left: 0px; padding-right: 0px; padding-top: 0px; width:121px; height:121px; background-color: #FFFFFF; border: 2px solid #993300;" |
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<div style="position:relative;"><div style="position:absolute; top:-1px;">[[Image:Berk-Icon-Chassis.png]]</div>&nbsp;</div>
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<b>[[BerkiGEM2007Present4 | The Chassis]]</b><p><i>Our bacterial chassis has been heavily modified to remove its sepsis-inducing toxicity, as well as promote its ability to last longer in the bloodstream by masking it from the immune system.  We performed this by lipopolysaccharide modifications as well as adding the K-capsule and O-antigen.</i></p>
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<div style="position:relative;"><div style="position:absolute; top:-1px;">[[Image:Berk-Icon-Controller.png]]</div>&nbsp;</div>
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<b>[[BerkiGEM2007Present3 | The Controller]]</b><p><i>The Controller is an integrated genetic circuit, comprised of two plasmids, that directs the initiation and production of the primary systems proteins through its component operons.  It is composed of a pSC101 controller plasmid and an amplifiable BAC utilizing the T7 RNAP expression system.</i></p>
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<div style="position:relative;"><div style="position:absolute; top:-1px;">[[Image:Berk-Icon-Self-Destruct.png]]</div>&nbsp;</div>
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<b>[[BerkiGEM2007Present5 | Genetic Self-Destruct]]</b><p><i>To prevent chance of infection or unwanted proliferation after hemoglobin production, we have engineered a genetic self-destruct mechanism whereby when induced, the bacterial cell will express a genetic material-degrading toxin which kills the cell, but leaves it physically intact.</i></p>
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|valign="top" style="padding-bottom: 3px; padding-left: 0px; padding-right: 0px; padding-top: 0px; width:121px; height:121px; background-color: #FFFFFF; border: 2px solid #993300;" |
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<div style="position:relative;"><div style="position:absolute; top:-1px;">[[Image:Berk-Icon-Freeze-Drying.png]]</div>&nbsp;</div>
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|style="padding: 10px; background-color: #FFFFFF; border: 2px solid #993300;"|
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<b>[[BerkiGEM2007Present2 | Freeze Drying]]</b><p><i>In order to enable preservation of our bacteria for long periods of time, we have included the ability to produce compounds such as hydroxyectoine that will enable our bacteria to survive freeze-drying intact.  This will dramatically increase shelf-life and decrease transport costs.</i></p>
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-->
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</style>
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----
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<p>
 +
<a href="/igem07/index.php/Image:Berkeley_BactobloodHeader.jpg" class="image" title="BactoBlood">
 +
<img src="/igem07/images/c/cd/Berkeley_BactobloodHeader.jpg" alt="BactoBlood" width="940" height="305" longdesc="BactoBlood Image" /></a><br />
 +
<br /></p>
 +
<p><b>The necessity</b> of cheap, available, disease free, and
 +
universally usable blood substitutes is undisputed. There are
 +
currently no blood substitutes approved for use in the US or the UK,
 +
and whole blood is almost always in short supply. Developing
 +
countries have the greatest need for blood transfusions, however
 +
many lack the necessary donation and storage infrastructure and the
 +
required number of healthy donors. To address this problem, we are
 +
developing an innovative and inexpensive blood substitute
 +
constructed from <i>E. coli</i> bacteria engineered to include the
 +
critical capabilities of human erythrocytes. Our bacterial system
 +
includes the ability to safely exist in the bloodstream, carry
 +
oxygen with hemoglobin, and be stored for prolonged periods in a
 +
freeze-dried state.<br /><br /></p>
 +
<p><b><i>Support for Berkeley iGEM 2007 was generously provided by
 +
SynBERC and The Camille and Henry Dreyfus Foundation, Inc.</i> </b>
 +
</p><hr />
 +
<p>&nbsp;</p>
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<p align="left">
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|-valign="top"
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<table width="100%" id="table2" border="2" cellpadding="0" style="padding: 0px; width: 780px; color: navyblue; background-color: lightblue;">
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|width=189.25px style="padding: 10px; background-color: lightblue; border: 2px solid #993300;" |
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<!--<tr>
 +
<td class="tdheadL">&nbsp;</td>
 +
<td class="tdheadR"><b>Project Modules</b></td>
 +
</tr>-->
 +
<tr>
 +
<td class="tdheadR" colspan="2">
 +
<h3>Project Modules</h3></td>
 +
</tr>
 +
<tr>
 +
<td class="tdboxes">
 +
<a href="https://2007.igem.org/BerkiGEM2007Present1"><img border="0" src="https://static.igem.org/mediawiki/2007/e/e5/Berk-Icon-Oxygen.png" width="121" height="121"></a></td>
 +
<td class="tddesc"><b><a href="https://2007.igem.org/BerkiGEM2007Present1">Oxygen Carrying</a></b><p><i>Our system is
 +
designed to produce Hemoglobin, Heme, and the necessary
 +
chaperones and detoxifying agents to promote the transport
 +
of oxygen throughout the bloodstream.&nbsp; We also
 +
investigated alternates to hemoglobin and other strategies
 +
for its production.</i></td>
 +
</tr>
 +
<tr>
 +
<td class="tdboxes">
 +
<a href="https://2007.igem.org/BerkiGEM2007Present4"><img border="0" src="https://static.igem.org/mediawiki/2007/b/ba/Berk-Icon-Chassis.png" width="121" height="121"></a></td>
 +
<td class="tddesc"><b><a href="https://2007.igem.org/BerkiGEM2007Present4">The Chassis</a></b><p><i>Our bacterial
 +
chassis has been heavily modified to remove its
 +
sepsis-inducing toxicity, as well as promote its ability to
 +
last longer in the bloodstream by masking it from the immune
 +
system.&nbsp; We performed this by lipopolysaccharide
 +
modifications as well as adding the K-capsule and O-antigen.</i></td>
 +
</tr>
 +
<tr>
 +
<td class="tdboxes">
 +
<a href="https://2007.igem.org/BerkiGEM2007Present3"><img border="0" src="https://static.igem.org/mediawiki/2007/8/8d/Berk-Icon-Controller.png" width="121" height="121"></a></td>
 +
<td class="tddesc"><b><a href="https://2007.igem.org/BerkiGEM2007Present3">The Controller</a></b><p><i>The Controller
 +
is an integrated genetic circuit, comprised of two plasmids,
 +
that directs the initiation and production of the primary
 +
systems proteins through its component operons.&nbsp; It is
 +
composed of a pSC101 controller plasmid and an amplifiable
 +
BAC utilizing the T7 RNAP expression system.</i></td>
 +
</tr>
 +
<tr>
 +
<td class="tdboxes">
 +
<a href="https://2007.igem.org/BerkiGEM2007Present5"><img border="0" src="https://static.igem.org/mediawiki/2007/c/c5/Berk-Icon-Self-Destruct.png" width="121" height="121"></a></td>
 +
<td class="tddesc"><b><a href="https://2007.igem.org/BerkiGEM2007Present5">Genetic Self-Destruct</a></b><p><i>To
 +
prevent chance of infection or unwanted proliferation after
 +
hemoglobin production, we have engineered a genetic
 +
self-destruct mechanism whereby when induced, the bacterial
 +
cell will express a genetic material-degrading toxin which
 +
kills the cell, but leaves it physically intact.</i></td>
 +
</tr>
 +
<tr>
 +
<td class="tdboxes">
 +
<a href="https://2007.igem.org/BerkiGEM2007Present2"><img border="0" src="https://static.igem.org/mediawiki/2007/c/ce/Berk-Icon-Freeze-Drying.png" width="121" height="121"></a></td>
 +
<td class="tddesc"><b><a href="https://2007.igem.org/BerkiGEM2007Present2">Freeze Drying</a></b><p><i>In order to
 +
enable preservation of our bacteria for long time periods,
 +
we have included the ability to produce compounds, such as
 +
hydroxyectoine, that will enable our bacteria to survive
 +
freeze-drying intact.&nbsp; This will dramatically increase
 +
shelf-life and decrease transport costs.</i></td>
 +
</tr>
 +
</table>
 +
<p><br /></p>
 +
<table width="100%" id="table3" border="2" style="padding: 0px; width: 780px; color: navyblue; background-color: #ffffaa;">
 +
<tr>
 +
<td class="tdheadR" style="background-color: #ffffaa;">
 +
<h3>Team Members</h3></td>
 +
</tr>
 +
<tr>
 +
<td class="tddesc"><b>Advisors</b><br />
 +
<a href="/igem07/index.php/John_Dueber" title="John Dueber">
 +
John Dueber</a> <font face="Arial">•</font>
 +
<a href="http://www.openwetware.org/wiki/User:JCAnderson" title="http://www.openwetware.org/wiki/User:JCAnderson" rel="nofollow">
 +
Christopher Anderson</a> <font face="Arial">•</font>
 +
<a href="http://genomics.lbl.gov/" title="http://genomics.lbl.gov/" rel="nofollow">
 +
Adam Arkin</a> <font face="Arial">•</font>
 +
<a href="https://keaslinglab.lbl.gov/wiki/index.php/Main_Page" title="https://keaslinglab.lbl.gov/wiki/index.php/Main Page" rel="nofollow">
 +
Jay Keasling</a><br />
 +
<p><b>Teaching Assistants</b><br />
 +
<a href="/igem07/index.php/Farnaz_Nowroozi" title="Farnaz Nowroozi">
 +
Farnaz Nowroozi</a> <font face="Arial">•</font>
 +
<a href="/igem07/index.php/Amin_Hajimorad" title="Amin Hajimorad">
 +
Amin Hajimorad</a> <font face="Arial">•</font>
 +
<a href="/igem07/index.php/Rickey_Bonds" title="Rickey Bonds">
 +
Rickey Bonds</a><br /></p>
 +
<p><b>Undergraduate Researchers</b><br />
 +
<a href="/igem07/index.php/Arthur_Yu" title="Arthur Yu">
 +
Arthur Yu</a> <font face="Arial">•</font>
 +
<a href="/igem07/index.php/Austin_Day" title="Austin Day">
 +
Austin Day</a> <font face="Arial">•</font>
 +
<a href="/igem07/index.php/David_Tulga" title="David Tulga">
 +
David Tulga</a> <font face="Arial">•</font>
 +
<a href="/igem07/index.php/Kristin_Doan" title="Kristin Doan">
 +
Kristin Doan</a> <font face="Arial">•</font>
 +
<a href="/igem07/index.php/Samantha_Liang" title="Samantha Liang">
 +
Samantha Liang</a> <font face="Arial">•</font>
 +
<a href="/igem07/index.php/Vaibhavi_Umesh" title="Vaibhavi Umesh">
 +
Vaibhavi Umesh</a> <font face="Arial">•</font>
 +
<a href="/igem07/index.php/Kristin_Fuller" title="Kristin Fuller">
 +
Kristin Fuller</a><br /></p>
 +
<p><b>High School Students</b><br />
 +
<a href="/igem07/index.php/Vincent_Parker" title="Vincent Parker">
 +
Vincent Parker</a> <font face="Arial">•</font>
 +
<a href="/igem07/index.php/Nhu_Nguyen" title="Nhu Nguyen">
 +
Nhu Nguyen</a> <font face="Arial">•</font>
 +
<a href="/igem07/index.php/Hannah_Cole" title="Hannah Cole">
 +
Hannah Cole</a></p></td>
 +
</tr>
 +
</table>
 +
<p><br /></p>
 +
<table cellspacing="2px" cellpadding="5" border="2" style="padding: 0px; width: 780px; color: navyblue; background-color: lightblue;">
 +
<tr valign="top">
 +
<td width="50%" style="padding:10px; background-color: #ffffaa; border: 2px solid #993300;">
 +
<div class="center">
 +
<a name="Team_Resources"></a>
 +
<h3>Team Resources</h3></div><hr />
 +
<p><br />
 +
<a href="/igem07/index.php/BerkiGEM2007Setup" title="BerkiGEM2007Setup">
 +
Presentation Setup</a></p>
 +
<p>
 +
<a href="http://spreadsheets.google.com/ccc?key=pUQEpr4ZqU9TxjeURaNm1Vw" class='external text' title="http://spreadsheets.google.com/ccc?key=pUQEpr4ZqU9TxjeURaNm1Vw" rel="nofollow">
 +
Oligo List Spreadsheet</a><br />
 +
<a href="http://spreadsheets.google.com/ccc?key=pUQEpr4ZqU9TerIU8gSvKbQ" class='external text' title="http://spreadsheets.google.com/ccc?key=pUQEpr4ZqU9TerIU8gSvKbQ" rel="nofollow">
 +
CloneSaver Spreadsheet</a><br />
 +
<a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=Berkeley_UC" class='external text' title="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=Berkeley UC" rel="nofollow">
 +
Our BioBrick Parts</a><br />
 +
<a href="/igem07/index.php/BerkiGEM2007_AllConstructionFiles" title="BerkiGEM2007 AllConstructionFiles">
 +
All construction files</a><br />
 +
<a href="/igem07/index.php/BerkiGEM2007_AllSequencingFiles" title="BerkiGEM2007 AllSequencingFiles">
 +
All sequencing files</a></p>
 +
<p><br /><b>Tools and Guides</b><br /></p>
 +
<p>
 +
<a href="http://www.openwetware.org/wiki/Arking:JCAOligoTutorialHome" class='external text' title="http://www.openwetware.org/wiki/Arking:JCAOligoTutorialHome" rel="nofollow">
 +
Biobricks and Cloning Tutorials</a><br />
 +
<a href="http://pir.georgetown.edu/pirwww/search/pairwise.shtml" class='external text' title="http://pir.georgetown.edu/pirwww/search/pairwise.shtml" rel="nofollow">
 +
Pairwise Alignment Online</a><br />
 +
<a href="http://align.genome.jp/" class='external text' title="http://align.genome.jp/" rel="nofollow">
 +
Multiple Sequence Alignment</a><br />
 +
<a href="http://en.wikipedia.org/wiki/Help:Wikitext_examples" class='external text' title="http://en.wikipedia.org/wiki/Help:Wikitext examples" rel="nofollow">
 +
Wiki Formatting Guide</a><br /></p>
 +
<p><br /><b>Useful Links</b> </p>
 +
<p>
 +
<a href="http://openwetware.org/wiki/IGEM:UC_Berkeley/2006" class='external text' title="http://openwetware.org/wiki/IGEM:UC Berkeley/2006" rel="nofollow">
 +
UC Berkeley iGEM 2006 OpenWetWare</a> <br />
 +
<a href="http://parts2.mit.edu/wiki/index.php/University_of_California_Berkeley_2006" class='external text' title="http://parts2.mit.edu/wiki/index.php/University of California Berkeley 2006" rel="nofollow">
 +
UC Berkeley iGEM 2006 wiki</a> <br />iGEM wikis:
 +
<a href="http://parts2.mit.edu/wiki/index.php/Main_Page" class='external text' title="http://parts2.mit.edu/wiki/index.php/Main Page" rel="nofollow">
 +
2006</a>,
 +
<a href="/igem07/index.php/Main_Page" title="Main Page">2007</a><br />
 +
<a href="http://partsregistry.org/Main_Page" class='external text' title="http://partsregistry.org/Main Page" rel="nofollow">
 +
Registry of Standard Biological Parts</a> <br />Biobricks
 +
Parts Lists:
 +
<a href="http://parts2.mit.edu/r/parts/partsdb/pgroup.cgi?pgroup=iGEM&amp;group=iGEM_Berkeley" class='external text' title="http://parts2.mit.edu/r/parts/partsdb/pgroup.cgi?pgroup=iGEM&amp;group=iGEM Berkeley" rel="nofollow">
 +
2005</a>,
 +
<a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2006&amp;group=Berkeley" class='external text' title="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2006&amp;group=Berkeley" rel="nofollow">
 +
2006</a>,
 +
<a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=Berkeley_UC" class='external text' title="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=Berkeley UC" rel="nofollow">
 +
2007</a><br />
 +
<a href="http://openwetware.org/wiki/Arking:JCAOligoTutorialHome" class='external text' title="http://openwetware.org/wiki/Arking:JCAOligoTutorialHome" rel="nofollow">
 +
Tutorials</a> <br /> </p></td>
 +
<td width="50%" style="padding: 10px; background-color: lightblue; border: 2px solid #993300;">
 +
<div class="center">
 +
<h3>Team Notebooks</h3></div><hr />
 +
<p><br />
 +
<a href="/igem07/index.php?title=John_Dueber_Notebook&amp;action=edit" class="new" title="John Dueber Notebook">
 +
John Dueber Notebook</a><br />
 +
<a href="/igem07/index.php/Christopher_Anderson_Notebook" title="Christopher Anderson Notebook">
 +
Christopher Anderson Notebook</a><br />
 +
<a href="/igem07/index.php?title=Farnaz_Nowroozi_Notebook&amp;action=edit" class="new" title="Farnaz Nowroozi Notebook">
 +
Farnaz Nowroozi Notebook</a><br />
 +
<a href="/igem07/index.php?title=Amin_Hajimorad_Notebook&amp;action=edit" class="new" title="Amin Hajimorad Notebook">
 +
Amin Hajimorad Notebook</a><br />
 +
<a href="/igem07/index.php/Rickey_Bonds_Notebook" title="Rickey Bonds Notebook">
 +
Rickey Bonds Notebook</a><br /></p>
 +
<p><br /><i>Keep your wiki notebooks,
 +
sequencing/construction logs, and the registry updated!</i>
 +
</p>
 +
<p><br />
 +
<a href="/igem07/index.php/Arthur_Yu_Notebook" title="Arthur Yu Notebook">
 +
Arthur Yu&#39;s 1337 Notebook</a><br />
 +
<a href="/igem07/index.php/Austin_Day_Notebook" title="Austin Day Notebook">
 +
Austin Day Notebook</a><br />
 +
<a href="/igem07/index.php/David_Tulga_Notebook" title="David Tulga Notebook">
 +
David Tulga&#39;s Notebook</a><br />
 +
<a href="/igem07/index.php/Kristin_Doan_Notebook" title="Kristin Doan Notebook">
 +
Kristin Doan Notebook</a><br />
 +
<a href="/igem07/index.php/Samantha_Liang_Notebook" title="Samantha Liang Notebook">
 +
Samantha&#39;s Notebook (June - July 19, 2007</a><br />
 +
<a href="/igem07/index.php/Samantha_Liang_Notebook2" title="Samantha Liang Notebook2">
 +
Samantha&#39;s Notebook (July 20, 2007 - present)</a><br />
 +
<a href="/igem07/index.php/Vaibhavi_Umesh_Notebook" title="Vaibhavi Umesh Notebook">
 +
Vaibhavi Umesh Notebook</a><br />
 +
<a href="/igem07/index.php/Kristin_Fuller_Notebook" title="Kristin Fuller Notebook">
 +
Kristin Fuller Notebook</a><br /></p>
 +
<p><br />
 +
<a href="/igem07/index.php/Vincent_Parker_Notebook" title="Vincent Parker Notebook">
 +
Vincent Parker Notebook</a><br />
 +
<a href="/igem07/index.php/Nhu_Nguyen_Notebook" title="Nhu Nguyen Notebook">
 +
Nhu Nguyen Notebook</a><br />
 +
<a href="/igem07/index.php/Hannah_Cole_Notebook" title="Hannah Cole Notebook">
 +
Hannah Cole Notebook</a></p></td>
 +
</tr>
 +
</table>
-
<center><h3>Team Members</h3></center>
 
-
----
+
</html>
-
 
+
-
 
+
-
'''Advisors'''<br>
+
-
[[John Dueber]]<br>
+
-
[http://www.openwetware.org/wiki/User:JCAnderson Christopher Anderson]<br>
+
-
[http://genomics.lbl.gov/ Adam Arkin]<br>
+
-
[https://keaslinglab.lbl.gov/wiki/index.php/Main_Page Jay Keasling]<br>
+
-
 
+
-
'''Teaching Assistants''' <br>
+
-
[[Farnaz Nowroozi]]<br>
+
-
[[Amin Hajimorad]]<br>
+
-
[[Rickey Bonds]]<br>
+
-
 
+
-
'''Undergraduate Researchers''' <br>
+
-
[[Arthur Yu]]<br>
+
-
[[Austin Day]]<br>
+
-
[[David Tulga]]<br>
+
-
[[Kristin Doan]]<br>
+
-
[[Samantha Liang]]<br>
+
-
[[Vaibhavi Umesh]]<br>
+
-
[[Kristin Fuller]]<br>
+
-
 
+
-
'''High School Students''' <br>
+
-
[[Vincent Parker]]<br>
+
-
[[Nhu Nguyen]]<br>
+
-
[[Hannah Cole]]<br>
+
-
 
+
-
|width=189.25px style="padding:10px; background-color: #ffffaa; border: 2px solid #993300;" |
+
-
 
+
-
<center><h3>Team Resources</h3></center>
+
-
 
+
-
----
+
-
 
+
-
 
+
-
[http://spreadsheets.google.com/ccc?key=pUQEpr4ZqU9TxjeURaNm1Vw Oligo List Spreadsheet]<br>
+
-
[http://spreadsheets.google.com/ccc?key=pUQEpr4ZqU9TerIU8gSvKbQ CloneSaver Spreadsheet]<br>
+
-
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Berkeley_UC Our BioBrick Parts]<br>
+
-
[[BerkiGEM2007_AllConstructionFiles | All construction files]]<br>
+
-
[[BerkiGEM2007_AllSequencingFiles | All sequencing files]]<br>
+
-
 
+
-
''If you need an invitation to the spreadsheets, ask Sam.''
+
-
 
+
-
 
+
-
'''Tools and Guides'''<br>
+
-
 
+
-
[http://www.openwetware.org/wiki/Arking:JCAOligoTutorialHome Biobricks and Cloning Tutorials]<br>
+
-
[http://pir.georgetown.edu/pirwww/search/pairwise.shtml Pairwise Alignment Online]<br>
+
-
[http://align.genome.jp/ Multiple Sequence Alignment]<br>
+
-
[http://en.wikipedia.org/wiki/Help:Wikitext_examples Wiki Formatting Guide]<br>
+
-
 
+
-
 
+
-
'''Useful Links'''
+
-
 
+
-
[http://openwetware.org/wiki/IGEM:UC_Berkeley/2006 UC Berkeley iGEM 2006 OpenWetWare] <br>
+
-
[http://parts2.mit.edu/wiki/index.php/University_of_California_Berkeley_2006 UC Berkeley iGEM 2006 wiki] <br>
+
-
iGEM wikis: [http://parts2.mit.edu/wiki/index.php/Main_Page 2006], [[Main_Page|2007]]<br>
+
-
[http://partsregistry.org/Main_Page Registry of Standard Biological Parts] <br>
+
-
Biobricks Parts Lists: [http://parts2.mit.edu/r/parts/partsdb/pgroup.cgi?pgroup=iGEM&group=iGEM_Berkeley 2005], [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2006&group=Berkeley 2006], [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Berkeley_UC 2007]<br>
+
-
[http://openwetware.org/wiki/Arking:JCAOligoTutorialHome Tutorials]
+
-
 
+
-
 
+
-
|width=300.25px style="padding: 10px; background-color: lightblue; border: 2px solid #993300;" |
+
-
 
+
-
<center><h3>Team Notebooks</h3></center>
+
-
 
+
-
----
+
-
 
+
-
 
+
-
[[John Dueber Notebook]]<br>
+
-
[[Christopher Anderson Notebook]]<br>
+
-
[[Farnaz Nowroozi Notebook]]<br>
+
-
[[Amin Hajimorad Notebook]]<br>
+
-
[[Rickey Bonds Notebook]]<br>
+
-
 
+
-
 
+
-
''Keep your wiki notebooks, sequencing/construction logs, and the registry updated!''
+
-
 
+
-
 
+
-
[[Arthur Yu Notebook | Arthur Yu's 1337 Notebook]]<br>
+
-
[[Austin Day Notebook]]<br>
+
-
[[David Tulga Notebook | David Tulga's Notebook]]<br>
+
-
[[Kristin Doan Notebook]]<br>
+
-
[[Samantha Liang Notebook | Samantha's Notebook (June - July 19, 2007]]<br>
+
-
[[Samantha Liang Notebook2 | Samantha's Notebook (July 20, 2007 - present)]]<br>
+
-
[[Vaibhavi Umesh Notebook]]<br>
+
-
[[Kristin Fuller Notebook]]<br>
+
-
 
+
-
 
+
-
[[Vincent Parker Notebook]]<br>
+
-
[[Nhu Nguyen Notebook]]<br>
+
-
[[Hannah Cole Notebook]]<br>
+
-
[[BerkiGEM2007Setup | Setup]]<br>
+
-
|}
+

Revision as of 02:40, 12 October 2007

BactoBlood

The necessity of cheap, available, disease free, and universally usable blood substitutes is undisputed. There are currently no blood substitutes approved for use in the US or the UK, and whole blood is almost always in short supply. Developing countries have the greatest need for blood transfusions, however many lack the necessary donation and storage infrastructure and the required number of healthy donors. To address this problem, we are developing an innovative and inexpensive blood substitute constructed from E. coli bacteria engineered to include the critical capabilities of human erythrocytes. Our bacterial system includes the ability to safely exist in the bloodstream, carry oxygen with hemoglobin, and be stored for prolonged periods in a freeze-dried state.

Support for Berkeley iGEM 2007 was generously provided by SynBERC and The Camille and Henry Dreyfus Foundation, Inc.

 

Project Modules
Oxygen Carrying

Our system is designed to produce Hemoglobin, Heme, and the necessary chaperones and detoxifying agents to promote the transport of oxygen throughout the bloodstream.  We also investigated alternates to hemoglobin and other strategies for its production.
The Chassis

Our bacterial chassis has been heavily modified to remove its sepsis-inducing toxicity, as well as promote its ability to last longer in the bloodstream by masking it from the immune system.  We performed this by lipopolysaccharide modifications as well as adding the K-capsule and O-antigen.
The Controller

The Controller is an integrated genetic circuit, comprised of two plasmids, that directs the initiation and production of the primary systems proteins through its component operons.  It is composed of a pSC101 controller plasmid and an amplifiable BAC utilizing the T7 RNAP expression system.
Genetic Self-Destruct

To prevent chance of infection or unwanted proliferation after hemoglobin production, we have engineered a genetic self-destruct mechanism whereby when induced, the bacterial cell will express a genetic material-degrading toxin which kills the cell, but leaves it physically intact.
Freeze Drying

In order to enable preservation of our bacteria for long time periods, we have included the ability to produce compounds, such as hydroxyectoine, that will enable our bacteria to survive freeze-drying intact.  This will dramatically increase shelf-life and decrease transport costs.


Team Members
Advisors
John Dueber • Christopher Anderson • Adam Arkin • Jay Keasling

Teaching Assistants
Farnaz Nowroozi • Amin Hajimorad • Rickey Bonds

Undergraduate Researchers
Arthur Yu • Austin Day • David Tulga • Kristin Doan • Samantha Liang • Vaibhavi Umesh • Kristin Fuller

High School Students
Vincent Parker • Nhu Nguyen • Hannah Cole


Team Resources


Presentation Setup

Oligo List Spreadsheet
CloneSaver Spreadsheet
Our BioBrick Parts
All construction files
All sequencing files


Tools and Guides

Biobricks and Cloning Tutorials
Pairwise Alignment Online
Multiple Sequence Alignment
Wiki Formatting Guide


Useful Links

UC Berkeley iGEM 2006 OpenWetWare
UC Berkeley iGEM 2006 wiki
iGEM wikis: 2006, 2007
Registry of Standard Biological Parts
Biobricks Parts Lists: 2005, 2006, 2007
Tutorials

Team Notebooks


John Dueber Notebook
Christopher Anderson Notebook
Farnaz Nowroozi Notebook
Amin Hajimorad Notebook
Rickey Bonds Notebook


Keep your wiki notebooks, sequencing/construction logs, and the registry updated!


Arthur Yu's 1337 Notebook
Austin Day Notebook
David Tulga's Notebook
Kristin Doan Notebook
Samantha's Notebook (June - July 19, 2007
Samantha's Notebook (July 20, 2007 - present)
Vaibhavi Umesh Notebook
Kristin Fuller Notebook


Vincent Parker Notebook
Nhu Nguyen Notebook
Hannah Cole Notebook

Retrieved from "http://2007.igem.org/wiki/index.php/Berkeley_UC"