Week 15

From 2007.igem.org

(Difference between revisions)
Line 1: Line 1:
::'''10/08/07'''
::'''10/08/07'''
*We inoculate:
*We inoculate:
-
::-I763031 (low copy plasmid);
+
::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
-
::-I763016 (low copy plasmid);
+
::-[http://partsregistry.org/Part:I763016 I763016](low copy plasmid);
::-E.Coli;
::-E.Coli;
-
::-I763032 (high copy plasmid).
+
::-[http://partsregistry.org/Part:I763032 I763032] (high copy plasmid).
*Digestion of:
*Digestion of:
-
::-I763035 with Eco/Pst1;
+
::-[http://partsregistry.org/Part:I763035 I763035] with Eco/Pst1;
-
::-I763007 with Eco/spe;
+
::-[http://partsregistry.org/Part:I763007 I763007] with Eco/spe;
-
::-I763026 with Eco/Xba;
+
::-[http://partsregistry.org/Part:I763026 I763026] with Eco/Xba;
::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Spe/Xba.
::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Spe/Xba.
*Ligation for:
*Ligation for:
-
::-I763035+pSB4A3;
+
::-[http://partsregistry.org/Part:I763035 I763035]+pSB4A3;
-
::-I763007+I763026;
+
::-[http://partsregistry.org/Part:I763007 I763007]+I763026;
-
::-I763007+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
+
::-[http://partsregistry.org/Part:I763007 I763007]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-
::-I763032+[http://partsregistry.org/Part:pSB4A3 pSB4A3].
+
::-[http://partsregistry.org/Part:I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3].
*Run on electrophoresis gel.
*Run on electrophoresis gel.
-
*I763007 and I763026 band extraction.
+
*[http://partsregistry.org/Part:I763007 I763007] and I763026 band extraction.
*O/N ligation for:
*O/N ligation for:
-
::-I763026+I763007;
+
::-I763026+[http://partsregistry.org/Part:I763007 I763007];
-
::-I763024+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
+
::-[http://partsregistry.org/Part:I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-
::-I763032+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
+
::-[http://partsregistry.org/Part:I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-
::-I763007+[http://partsregistry.org/Part:pSB4A3 pSB4A3].
+
::-[http://partsregistry.org/Part:I763007 I763007]+[http://partsregistry.org/Part:pSB4A3 pSB4A3].
*We inoculate:
*We inoculate:
-
::-I763031 (low copy plasmid);
+
::-[http://partsregistry.org/Part:I763031 I763031](low copy plasmid);
-
::-I763016 (low copy plasmid);
+
::-[http://partsregistry.org/Part:I763016 I763016](low copy plasmid);
-
::-I763032 (high copy plasmid).
+
::-[http://partsregistry.org/Part:I763032 I763032] (high copy plasmid).
Line 31: Line 31:
::'''10/09/07'''
::'''10/09/07'''
*Transformation of:
*Transformation of:
-
::-I763026 + I763007;
+
::-I763026 + [http://partsregistry.org/Part:I763007 I763007];
-
::-I763024 (low copy plasmid);
+
::-[http://partsregistry.org/Part:I763024 I763024] (low copy plasmid);
-
::-I763032 (low copy plasmid);
+
::-[http://partsregistry.org/Part:I763032 I763032] (low copy plasmid);
-
::-I763007 (low copy plasmid).
+
::-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid).
*We inoculate in 6ml:
*We inoculate in 6ml:
-
::-I763031 (low copy plasmid);
+
::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
-
::-I763016 (low copy plasmid).
+
::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid).
*Ligation for:
*Ligation for:
-
::-I763016 (Eco/Spe)+GFP.(Eco/Xba).
+
::-[http://partsregistry.org/Part:I763016 I763016] (Eco/Spe)+GFP.(Eco/Xba).
::'''10/10/07'''
::'''10/10/07'''
*Miniprep of:
*Miniprep of:
-
::-I763016 (low copy plasmid);
+
::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid);
-
::-I763031(low copy plasmid).
+
::-[http://partsregistry.org/Part:I763031 I763031](low copy plasmid).
*Digestion for :
*Digestion for :
-
::-I763031 with Xba/Pst1;
+
::-[http://partsregistry.org/Part:I763031 I763031] with Xba/Pst1;
-
::-I763016 (low copy plasmid) with Spe/Pst1;
+
::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid) with Spe/Pst1;
*Run on electrophoresis gel and I763016 band extraction.
*Run on electrophoresis gel and I763016 band extraction.
*Ligation for :
*Ligation for :
-
::-I763016 (low copy plasmid)+GFP;
+
::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid)+GFP;
-
::-I763024+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
+
::-[http://partsregistry.org/Part:I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-
::-I763024+C0012(high copy plasmid).
+
::-[http://partsregistry.org/Part:I763024 I763024]+C0012(high copy plasmid).
*We inoculate in 6ml:
*We inoculate in 6ml:
-
::-I763031 (low copy plasmid);
+
::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
-
::-I763016 (low copy plasmid);
+
::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid);
::-I763026 + I763007(high copy plasmid);
::-I763026 + I763007(high copy plasmid);
-
::-I763007 (low copy plasmid);
+
::-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid);
-
::-I763024(low copy plasmid).
+
::-[http://partsregistry.org/Part:I763024 I763024](low copy plasmid).
::'''10/11/07'''
::'''10/11/07'''
*Stock preparation (500ul) of:
*Stock preparation (500ul) of:
-
::-I763031 (low copy plasmid);
+
::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
-
::-I763016 (low copy plasmid);
+
::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid);
::-I763026 + I763007(high copy plasmid);
::-I763026 + I763007(high copy plasmid);
-
::-I763007 (low copy plasmid);
+
::-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid);
-
::-I763024(low copy plasmid).
+
::-[http://partsregistry.org/Part:I763024 I763024](low copy plasmid).
*Miniprep of:
*Miniprep of:
-
::-I763031 (low copy plasmid);
+
::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
-
::-I763016 (low copy plasmid);
+
::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid);
::-I763026 + I763007(high copy plasmid);
::-I763026 + I763007(high copy plasmid);
-
::-I763007 (low copy plasmid);
+
::-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid);
-
::-I763024(low copy plasmid).
+
::-[http://partsregistry.org/Part:I763024 I763024](low copy plasmid).
*Fluorescence test of:
*Fluorescence test of:
-
::-I763031 (low copy plasmid);
+
::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
::-I763026 + I763007(high copy plasmid);
::-I763026 + I763007(high copy plasmid);
::-I763007 (low copy plasmid).
::-I763007 (low copy plasmid).
*Control digestion of:
*Control digestion of:
-
::-I763031 with Xba/Pst1;
+
::-[http://partsregistry.org/Part:I763031 I763031] with Xba/Pst1;
-
::-I763016 with Xba/Pst1 and Spe/Xba;
+
::-[http://partsregistry.org/Part:I763016 I763016] with Xba/Pst1 and Spe/Xba;
::-I763026 + I763007 with Xba/Pst1 and Spe/Xba;
::-I763026 + I763007 with Xba/Pst1 and Spe/Xba;
-
::-I763024 with Xba/Pst1;
+
::-[http://partsregistry.org/Part:I763024 I763024] with Xba/Pst1;
-
::-I763007 with Xba/Pst1.
+
::-[http://partsregistry.org/Part:I763007 I763007] with Xba/Pst1.
*Run on electrophoresis gel and band extraction.
*Run on electrophoresis gel and band extraction.
*Transformation of:
*Transformation of:
-
::-I763019;
+
::-[http://partsregistry.org/Part:I763019 I763019];
-
::-I763024+C0012;
+
::-[http://partsregistry.org/Part:I763024 I763024]+C0012;
-
::-I763032.
+
::-[http://partsregistry.org/Part:I763032 I763032].
*We inoculate  
*We inoculate  
-
::-I763019.     
+
::-[http://partsregistry.org/Part:I763019 I763019].     
::'''10/12/07'''
::'''10/12/07'''
*Miniprep of:
*Miniprep of:
-
::-I763019.
+
::-[http://partsregistry.org/Part:I763019 I763019].
*Digestion of:
*Digestion of:
-
::-I763019 with Xba/Pst1;
+
::-[http://partsregistry.org/Part:I763019 I763019] with Xba/Pst1;
-
::-I763019 with Eco/Xba.
+
::-[http://partsregistry.org/Part:I763019 I763019] with Eco/Xba.
*Run on electrophoresis gel.
*Run on electrophoresis gel.

Revision as of 10:39, 12 October 2007

10/08/07
  • We inoculate:
-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
-[http://partsregistry.org/Part:I763016 I763016](low copy plasmid);
-E.Coli;
-[http://partsregistry.org/Part:I763032 I763032] (high copy plasmid).
  • Digestion of:
-[http://partsregistry.org/Part:I763035 I763035] with Eco/Pst1;
-[http://partsregistry.org/Part:I763007 I763007] with Eco/spe;
-[http://partsregistry.org/Part:I763026 I763026] with Eco/Xba;
-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Spe/Xba.
  • Ligation for:
-[http://partsregistry.org/Part:I763035 I763035]+pSB4A3;
-[http://partsregistry.org/Part:I763007 I763007]+I763026;
-[http://partsregistry.org/Part:I763007 I763007]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-[http://partsregistry.org/Part:I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3].
  • Run on electrophoresis gel.
  • [http://partsregistry.org/Part:I763007 I763007] and I763026 band extraction.
  • O/N ligation for:
-I763026+[http://partsregistry.org/Part:I763007 I763007];
-[http://partsregistry.org/Part:I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-[http://partsregistry.org/Part:I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-[http://partsregistry.org/Part:I763007 I763007]+[http://partsregistry.org/Part:pSB4A3 pSB4A3].
  • We inoculate:
-[http://partsregistry.org/Part:I763031 I763031](low copy plasmid);
-[http://partsregistry.org/Part:I763016 I763016](low copy plasmid);
-[http://partsregistry.org/Part:I763032 I763032] (high copy plasmid).


10/09/07
  • Transformation of:
-I763026 + [http://partsregistry.org/Part:I763007 I763007];
-[http://partsregistry.org/Part:I763024 I763024] (low copy plasmid);
-[http://partsregistry.org/Part:I763032 I763032] (low copy plasmid);
-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid).
  • We inoculate in 6ml:
-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid).
  • Ligation for:
-[http://partsregistry.org/Part:I763016 I763016] (Eco/Spe)+GFP.(Eco/Xba).


10/10/07
  • Miniprep of:
-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid);
-[http://partsregistry.org/Part:I763031 I763031](low copy plasmid).
  • Digestion for :
-[http://partsregistry.org/Part:I763031 I763031] with Xba/Pst1;
-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid) with Spe/Pst1;
  • Run on electrophoresis gel and I763016 band extraction.
  • Ligation for :
-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid)+GFP;
-[http://partsregistry.org/Part:I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-[http://partsregistry.org/Part:I763024 I763024]+C0012(high copy plasmid).
  • We inoculate in 6ml:
-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid);
-I763026 + I763007(high copy plasmid);
-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid);
-[http://partsregistry.org/Part:I763024 I763024](low copy plasmid).
10/11/07
  • Stock preparation (500ul) of:
-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid);
-I763026 + I763007(high copy plasmid);
-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid);
-[http://partsregistry.org/Part:I763024 I763024](low copy plasmid).
  • Miniprep of:
-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid);
-I763026 + I763007(high copy plasmid);
-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid);
-[http://partsregistry.org/Part:I763024 I763024](low copy plasmid).
  • Fluorescence test of:
-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
-I763026 + I763007(high copy plasmid);
-I763007 (low copy plasmid).
  • Control digestion of:
-[http://partsregistry.org/Part:I763031 I763031] with Xba/Pst1;
-[http://partsregistry.org/Part:I763016 I763016] with Xba/Pst1 and Spe/Xba;
-I763026 + I763007 with Xba/Pst1 and Spe/Xba;
-[http://partsregistry.org/Part:I763024 I763024] with Xba/Pst1;
-[http://partsregistry.org/Part:I763007 I763007] with Xba/Pst1.
  • Run on electrophoresis gel and band extraction.
  • Transformation of:
-[http://partsregistry.org/Part:I763019 I763019];
-[http://partsregistry.org/Part:I763024 I763024]+C0012;
-[http://partsregistry.org/Part:I763032 I763032].
  • We inoculate
-[http://partsregistry.org/Part:I763019 I763019].
10/12/07
  • Miniprep of:
-[http://partsregistry.org/Part:I763019 I763019].
  • Digestion of:
-[http://partsregistry.org/Part:I763019 I763019] with Xba/Pst1;
-[http://partsregistry.org/Part:I763019 I763019] with Eco/Xba.
  • Run on electrophoresis gel.


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