Week 15
From 2007.igem.org
(Difference between revisions)
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*[http://partsregistry.org/Part:I763007 I763007] and I763026 band extraction. | *[http://partsregistry.org/Part:I763007 I763007] and I763026 band extraction. | ||
*O/N ligation for: | *O/N ligation for: | ||
| - | ::-I763026+[http://partsregistry.org/Part:I763007 I763007]; | + | ::-[http://partsregistry.org/Part:I763026 I763026]+[http://partsregistry.org/Part:I763007 I763007]; |
::-[http://partsregistry.org/Part:I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3]; | ::-[http://partsregistry.org/Part:I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3]; | ||
::-[http://partsregistry.org/Part:I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3]; | ::-[http://partsregistry.org/Part:I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3]; | ||
| Line 31: | Line 31: | ||
::'''10/09/07''' | ::'''10/09/07''' | ||
*Transformation of: | *Transformation of: | ||
| - | ::-I763026 + [http://partsregistry.org/Part:I763007 I763007]; | + | ::-[http://partsregistry.org/Part:I763026 I763026] + [http://partsregistry.org/Part:I763007 I763007]; |
::-[http://partsregistry.org/Part:I763024 I763024] (low copy plasmid); | ::-[http://partsregistry.org/Part:I763024 I763024] (low copy plasmid); | ||
::-[http://partsregistry.org/Part:I763032 I763032] (low copy plasmid); | ::-[http://partsregistry.org/Part:I763032 I763032] (low copy plasmid); | ||
| Line 57: | Line 57: | ||
::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid); | ::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid); | ||
::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid); | ::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid); | ||
| - | ::-I763026 + I763007(high copy plasmid); | + | ::-[http://partsregistry.org/Part:I763026 I763026] + [http://partsregistry.org/Part:I763007 I763007](high copy plasmid); |
::-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid); | ::-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid); | ||
::-[http://partsregistry.org/Part:I763024 I763024](low copy plasmid). | ::-[http://partsregistry.org/Part:I763024 I763024](low copy plasmid). | ||
| Line 64: | Line 64: | ||
::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid); | ::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid); | ||
::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid); | ::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid); | ||
| - | ::-I763026 + I763007(high copy plasmid); | + | ::-[http://partsregistry.org/Part:I763026 I763026] + [http://partsregistry.org/Part:I763007 I763007](high copy plasmid); |
::-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid); | ::-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid); | ||
::-[http://partsregistry.org/Part:I763024 I763024](low copy plasmid). | ::-[http://partsregistry.org/Part:I763024 I763024](low copy plasmid). | ||
| Line 70: | Line 70: | ||
::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid); | ::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid); | ||
::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid); | ::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid); | ||
| - | ::-I763026 + I763007(high copy plasmid); | + | ::-[http://partsregistry.org/Part:I763026 I763026]+ [http://partsregistry.org/Part:I763007 I763007](high copy plasmid); |
::-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid); | ::-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid); | ||
::-[http://partsregistry.org/Part:I763024 I763024](low copy plasmid). | ::-[http://partsregistry.org/Part:I763024 I763024](low copy plasmid). | ||
*Fluorescence test of: | *Fluorescence test of: | ||
::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid); | ::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid); | ||
| - | ::-I763026 + I763007(high copy plasmid); | + | ::-[http://partsregistry.org/Part:I763026 I763026] + [http://partsregistry.org/Part:I763007 I763007](high copy plasmid); |
| - | ::-I763007 (low copy plasmid). | + | ::-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid). |
*Control digestion of: | *Control digestion of: | ||
::-[http://partsregistry.org/Part:I763031 I763031] with Xba/Pst1; | ::-[http://partsregistry.org/Part:I763031 I763031] with Xba/Pst1; | ||
::-[http://partsregistry.org/Part:I763016 I763016] with Xba/Pst1 and Spe/Xba; | ::-[http://partsregistry.org/Part:I763016 I763016] with Xba/Pst1 and Spe/Xba; | ||
| - | ::-I763026 + I763007 with Xba/Pst1 and Spe/Xba; | + | ::-[http://partsregistry.org/Part:I763026 I763026] + [http://partsregistry.org/Part:I763007 I763007] with Xba/Pst1 and Spe/Xba; |
::-[http://partsregistry.org/Part:I763024 I763024] with Xba/Pst1; | ::-[http://partsregistry.org/Part:I763024 I763024] with Xba/Pst1; | ||
::-[http://partsregistry.org/Part:I763007 I763007] with Xba/Pst1. | ::-[http://partsregistry.org/Part:I763007 I763007] with Xba/Pst1. | ||
| Line 86: | Line 86: | ||
*Transformation of: | *Transformation of: | ||
::-[http://partsregistry.org/Part:I763019 I763019]; | ::-[http://partsregistry.org/Part:I763019 I763019]; | ||
| - | ::-[http://partsregistry.org/Part:I763024 I763024]+C0012; | + | ::-[http://partsregistry.org/Part:I763024 I763024]+C0012[http://partsregistry.org/Part:C0012 C0012]; |
::-[http://partsregistry.org/Part:I763032 I763032]. | ::-[http://partsregistry.org/Part:I763032 I763032]. | ||
*We inoculate | *We inoculate | ||
Revision as of 10:42, 12 October 2007
- 10/08/07
- We inoculate:
- -[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
- -[http://partsregistry.org/Part:I763016 I763016](low copy plasmid);
- -E.Coli;
- -[http://partsregistry.org/Part:I763032 I763032] (high copy plasmid).
- Digestion of:
- -[http://partsregistry.org/Part:I763035 I763035] with Eco/Pst1;
- -[http://partsregistry.org/Part:I763007 I763007] with Eco/spe;
- -[http://partsregistry.org/Part:I763026 I763026] with Eco/Xba;
- -[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Spe/Xba.
- Ligation for:
- -[http://partsregistry.org/Part:I763035 I763035]+pSB4A3;
- -[http://partsregistry.org/Part:I763007 I763007]+I763026;
- -[http://partsregistry.org/Part:I763007 I763007]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
- -[http://partsregistry.org/Part:I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3].
- Run on electrophoresis gel.
- [http://partsregistry.org/Part:I763007 I763007] and I763026 band extraction.
- O/N ligation for:
- -[http://partsregistry.org/Part:I763026 I763026]+[http://partsregistry.org/Part:I763007 I763007];
- -[http://partsregistry.org/Part:I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
- -[http://partsregistry.org/Part:I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
- -[http://partsregistry.org/Part:I763007 I763007]+[http://partsregistry.org/Part:pSB4A3 pSB4A3].
- We inoculate:
- -[http://partsregistry.org/Part:I763031 I763031](low copy plasmid);
- -[http://partsregistry.org/Part:I763016 I763016](low copy plasmid);
- -[http://partsregistry.org/Part:I763032 I763032] (high copy plasmid).
- 10/09/07
- Transformation of:
- -[http://partsregistry.org/Part:I763026 I763026] + [http://partsregistry.org/Part:I763007 I763007];
- -[http://partsregistry.org/Part:I763024 I763024] (low copy plasmid);
- -[http://partsregistry.org/Part:I763032 I763032] (low copy plasmid);
- -[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid).
- We inoculate in 6ml:
- -[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
- -[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid).
- Ligation for:
- -[http://partsregistry.org/Part:I763016 I763016] (Eco/Spe)+GFP.(Eco/Xba).
- 10/10/07
- Miniprep of:
- -[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid);
- -[http://partsregistry.org/Part:I763031 I763031](low copy plasmid).
- Digestion for :
- -[http://partsregistry.org/Part:I763031 I763031] with Xba/Pst1;
- -[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid) with Spe/Pst1;
- Run on electrophoresis gel and I763016 band extraction.
- Ligation for :
- -[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid)+GFP;
- -[http://partsregistry.org/Part:I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
- -[http://partsregistry.org/Part:I763024 I763024]+C0012(high copy plasmid).
- We inoculate in 6ml:
- -[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
- -[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid);
- -[http://partsregistry.org/Part:I763026 I763026] + [http://partsregistry.org/Part:I763007 I763007](high copy plasmid);
- -[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid);
- -[http://partsregistry.org/Part:I763024 I763024](low copy plasmid).
- 10/11/07
- Stock preparation (500ul) of:
- -[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
- -[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid);
- -[http://partsregistry.org/Part:I763026 I763026] + [http://partsregistry.org/Part:I763007 I763007](high copy plasmid);
- -[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid);
- -[http://partsregistry.org/Part:I763024 I763024](low copy plasmid).
- Miniprep of:
- -[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
- -[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid);
- -[http://partsregistry.org/Part:I763026 I763026]+ [http://partsregistry.org/Part:I763007 I763007](high copy plasmid);
- -[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid);
- -[http://partsregistry.org/Part:I763024 I763024](low copy plasmid).
- Fluorescence test of:
- -[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
- -[http://partsregistry.org/Part:I763026 I763026] + [http://partsregistry.org/Part:I763007 I763007](high copy plasmid);
- -[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid).
- Control digestion of:
- -[http://partsregistry.org/Part:I763031 I763031] with Xba/Pst1;
- -[http://partsregistry.org/Part:I763016 I763016] with Xba/Pst1 and Spe/Xba;
- -[http://partsregistry.org/Part:I763026 I763026] + [http://partsregistry.org/Part:I763007 I763007] with Xba/Pst1 and Spe/Xba;
- -[http://partsregistry.org/Part:I763024 I763024] with Xba/Pst1;
- -[http://partsregistry.org/Part:I763007 I763007] with Xba/Pst1.
- Run on electrophoresis gel and band extraction.
- Transformation of:
- -[http://partsregistry.org/Part:I763019 I763019];
- -[http://partsregistry.org/Part:I763024 I763024]+C0012[http://partsregistry.org/Part:C0012 C0012];
- -[http://partsregistry.org/Part:I763032 I763032].
- We inoculate
- -[http://partsregistry.org/Part:I763019 I763019].
- 10/12/07
- Miniprep of:
- -[http://partsregistry.org/Part:I763019 I763019].
- Digestion of:
- -[http://partsregistry.org/Part:I763019 I763019] with Xba/Pst1;
- -[http://partsregistry.org/Part:I763019 I763019] with Eco/Xba.
- Run on electrophoresis gel.
