McGill
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<u>'''Quorum-sensing coupled with the Repressilator'''</u> | <u>'''Quorum-sensing coupled with the Repressilator'''</u> | ||
| - | Our project is a continuation of one of the projects we presented last year: a two-gene oscillator, with an 'On' switch - LuxI gene, and an 'Off' switch - LacI, expressing a Cyan fluorescent protein when turned on for visualization. This simple relaxation oscillator makes a robust system of oscillations that can act as a 'pace maker' for more complicated oscillating systems. Quorum-sensing coupling between the systems is achieved with a diffusible artificial inducer (AI) protein made from LuxI which couples when produced, to a constitutively expressed gene in the system LuxR which can turn 'on' the system by promoting the synthesis of Lac from pLux. As Lac accumulates, it acts to turn 'off' the system by repressing the pLac controlling the synthesis of LuxI itself. Also, once AI is produced, it can easily diffuse to other cells and continue this 'On'-'Off' oscillator in other neighbouring cells, and hence increasing synchronization across a population of oscillating cells. This year we' | + | Our project is a continuation of one of the projects we presented last year: a two-gene oscillator, with an 'On' switch - LuxI gene, and an 'Off' switch - LacI, expressing a Cyan fluorescent protein when turned on for visualization. This simple relaxation oscillator makes a robust system of oscillations that can act as a 'pace maker' for more complicated oscillating systems. Quorum-sensing coupling between the systems is achieved with a diffusible artificial inducer (AI) protein made from LuxI which couples when produced, to a constitutively expressed gene in the system LuxR which can turn 'on' the system by promoting the synthesis of Lac from pLux. As Lac accumulates, it acts to turn 'off' the system by repressing the pLac controlling the synthesis of LuxI itself. Also, once AI is produced, it can easily diffuse to other cells and continue this 'On'-'Off' oscillator in other neighbouring cells, and hence increasing synchronization across a population of oscillating cells. <br> |
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| + | This year we've taken this system even further... [[McGill/Project Overview|learn more]]<br><br> | ||
[[McGill/Project Description|Project Description]]<br> | [[McGill/Project Description|Project Description]]<br> | ||
Revision as of 21:16, 14 October 2007
Project OverviewQuorum-sensing coupled with the Repressilator Our project is a continuation of one of the projects we presented last year: a two-gene oscillator, with an 'On' switch - LuxI gene, and an 'Off' switch - LacI, expressing a Cyan fluorescent protein when turned on for visualization. This simple relaxation oscillator makes a robust system of oscillations that can act as a 'pace maker' for more complicated oscillating systems. Quorum-sensing coupling between the systems is achieved with a diffusible artificial inducer (AI) protein made from LuxI which couples when produced, to a constitutively expressed gene in the system LuxR which can turn 'on' the system by promoting the synthesis of Lac from pLux. As Lac accumulates, it acts to turn 'off' the system by repressing the pLac controlling the synthesis of LuxI itself. Also, once AI is produced, it can easily diffuse to other cells and continue this 'On'-'Off' oscillator in other neighbouring cells, and hence increasing synchronization across a population of oscillating cells. This year we've taken this system even further... learn more Project Description
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