PHO80cds

From 2007.igem.org

Revision as of 10:38, 17 October 2007

Gene Pho80 CODING SEQUENCE

1 ATGGAAAGCA CATCAGGAGA ACGTTCCGAA AATATACATG AGGATCAAGG

51 GATACCAAAA GTAATTCTGC CCGCTGATTT TAATAAATGC TCTAGAACTG

101 ACCTAGTGGT GCTCATATCA CGAATGTTAG TATCGCTGAT AGCAATCAAT

151 GAAAATTCAG CAACAAAGAA ATCTGATGAC CAAATTACTT TAACACGATA

201 CCATTCTAAG ATTCCTCCAA ACATATCAAT CTTCAACTAT TTCATACGAC

251 TGACAAAGTT TTCCTCTTTA GAACATTGTG TGCTTATGAC ATCACTCTAT

301 TATATCGATT TATTGCAAAC TGTGTATCCT GATTTTACGC TTAATTCGTT

351 GACTGCCCAT AGGTTTTTAT TAACAGCCAC CACAGTCGCA ACAAAAGGCT

401 TATGTGATTC GTTCTCAACA AACGCCCATT ATGCAAAAGT TGGAGGAGTA

451 CGATGTCACG AATTGAATAT ACTGGAGAAC GATTTTTTAA AGAGAGTAAA

501 CTACAGAATC ATTCCGCGGG ATCATAACAT TACGTTATGT AGTATAGAGC

551 AAAAACAGAA AAAGTTTGTC ATAGATAAAA ACGCATTAGG GTCTCTCGAT

601 TTGGATTCTT ATTCTTACGT TAATCGTCCA AAAAGTGGAT ATAATGTTCT

651 AGATAAATAC TATCGAAGAA TAGTTCAGCT GGTGGGTTCC TTTAACGCTT

701 CACCTGATAA GAGTAGAAAG GTTGATTATG TTCTCCCGCC AAATATTGAT

751 ATAGTGAGTG AAAGTGGCTC CCAAACTACT CAACTAAAGG GGTCGTCATC

801 ACCCAATTCT CACTCTTCAC AAAAGCGATA TTCTGAGGCA AAGGACGCAC

851 ATATCTATAA CAAGCGATCA AAGCCAGATT AA

XbaI cuts in 2 sites 91 and 648

Pho80cds 882 bp e 293aa

XbaI restriction sequence TCTAGA

1)91 bp position 31-32aa SR S=TCT TCC TCA TCG

R=AGA AGG

2)648 bp position 216-217-218aa VLD V=TT GTC GTA GTG

L=TTA TTG CTT CTA CTC CTG

D=GAT GAC

5'3' Frame 1

Met E S T S G E R S E N I H E D Q G I P K V I L P A D F N K C S R T D L V V L I S R Met L V S L I A I N E N S A T K K S D D Q I T L T R Y H S K I P P N I S I F N Y F I R L T K F S S L E H C V L Met T S L Y Y I D L L Q T V Y P D F T L N S L T A H R F L L T A T T V A T K G L C D S F S T N A H Y A K V G G V R C H E L N I L E N D F L K R V N Y R I I P R D H N I T L C S I E Q K Q K K F V I D K N A L G S L D L D S Y S Y V N R P K S G Y N V L D K Y Y R R I V Q L V G S F N A S P D K S R K V D Y V L P P N I D I V S E S G S Q T T Q L K G S S S P N S H S S Q K R Y S E A K D A H I Y N K R S K P D Stop

Virtual Sequence: SEQUENCE 293 AA; 33227 MW; 72918CAF845AE74C CRC64.

    MESTSGERSE NIHEDQGIPK VILPADFNKC SRTDLVVLIS RMLVSLIAIN ENSATKKSDD
    QITLTRYHSK IPPNISIFNY FIRLTKFSSL EHCVLMTSLY YIDLLQTVYP DFTLNSLTAH
    RFLLTATTVA TKGLCDSFST NAHYAKVGGV RCHELNILEN DFLKRVNYRI IPRDHNITLC
    SIEQKQKKFV IDKNALGSLD LDSYSYVNRP KSGYNVLDKY YRRIVQLVGS FNASPDKSRK
    VDYVLPPNID IVSESGSQTT QLKGSSSPNS HSSQKRYSEA KDAHIYNKRS KPD

PHO80 Primer sequences:

t93c 5'-cgctgattttaataaatgctccagaactgacctagtggtgc-3'

t93c antisense 5'-gcaccactaggtcagttctggagcatttattaaaatcagcg-3'

t648c 5'-atcgtccaaaaagtggatataatgtcctagataaatactatcgaagaatag-3'

t648c antisense 5'-ctattcttcgatagtatttatctaggacattatatccactttttggacgat-3'