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Revision as of 13:34, 20 September 2007 (view source) Robertina (Talk | contribs) ← Older edit		Revision as of 08:48, 19 October 2007 (view source) Alda (Talk | contribs) Newer edit →
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	5’- CGC GAGCTC  TTAAAGCTTCTTTCCGCCCTT-3’		5’- CGC GAGCTC  TTAAAGCTTCTTTCCGCCCTT-3’
	Tm 60°C		Tm 60°C
		+
		+	PHO 80 fw Bam HI
		+	5’- CGC GGATCC ATGGAAAGCACATCAGGAGA-3’
		+
		+	PHO 80 rev Sac I
		+	5’- CGC GAGCTC  TTAATCTGGCTTTGATCGCT-3’

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Revision as of 08:48, 19 October 2007

We have design two oligo primers for 2Xore,1ORE and Luc with our restriction enzyme to amplify from plasmid p2UG-2XOre-Luc:

2X ORE – CYC1 Hind III ORE Fw 5’-CCC AAGCTT TAGTCAATAATGGGAGCTGG-3’ Tm 62°C

2X ORE – CYC1 BamHI Rev 5’-CGC GGATCC GTGCGTTTGTGGCGCCCTTA-3’ Tm 68°C

1X ORE – CYC1 Hind III ORE Fw 5’-CCC AAGCTT GGCTCTACCGAGCTGGTAGA-3’

1X ORE – CYC1 BamHI Rev 5’-CGC GGATCC GTGCGTTTGTGGCGCCCTTA-3’

LUCIFERASE BamH1 Fv 5’- CGC GGATCC ATGGAAGACGCCAAAAACATAA-3’ Tm 60°C

LUCIFERASE SacI Rv 5’- CGC GAGCTC TTAAAGCTTCTTTCCGCCCTT-3’ Tm 60°C

PHO 80 fw Bam HI 5’- CGC GGATCC ATGGAAAGCACATCAGGAGA-3’

PHO 80 rev Sac I 5’- CGC GAGCTC TTAATCTGGCTTTGATCGCT-3’