BerkiGEM2007-SamanthaConstructionFile8

From 2007.igem.org

(Difference between revisions)
Line 1: Line 1:
-
'''[lox][term][lox]''' <br>
+
'''[rbs][ATG][lox][term][lox]''' <br>
'''2 Different terminators (1124 and 1092 - will probably use 1092 in the end)''' <br>
'''2 Different terminators (1124 and 1092 - will probably use 1092 in the end)''' <br>
 +
'''using Bca1090 rbs site''' <br>
----
----
-
Digest pBca9145-I716205 (BamHI/AlwnI, 1723+553bp, Large) <br>
+
Digest pBca9145-I716204 (BamHI/AlwnI, 1547+553bp, Large) <br>
-
Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small) <br>
+
Digest pBca9145-I716207 (BglII/AlwnI, 1519+797bp, Small) <br>
-
Product is pBca9145-I716207. <br>
+
Product is pBca9145-I7162014. <br>
-
Digest pBca9145-I716206 (BamHI/AlwnI, 1694+553bp, Large) <br>
+
Digest pBca9145-I716204 (BamHI/AlwnI, 1547+553bp, Large) <br>
-
Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small) <br>
+
Digest pBca9145-I716208 (BglII/AlwnI, 1519+768bp, Small) <br>
-
Product is pBca9145-I716208. <br>
+
Product is pBca9145-I7162015. <br>

Revision as of 00:12, 8 June 2007

[rbs][ATG][lox][term][lox]
2 Different terminators (1124 and 1092 - will probably use 1092 in the end)
using Bca1090 rbs site


Digest pBca9145-I716204 (BamHI/AlwnI, 1547+553bp, Large)
Digest pBca9145-I716207 (BglII/AlwnI, 1519+797bp, Small)
Product is pBca9145-I7162014.


Digest pBca9145-I716204 (BamHI/AlwnI, 1547+553bp, Large)
Digest pBca9145-I716208 (BglII/AlwnI, 1519+768bp, Small)
Product is pBca9145-I7162015.

Retrieved from "http://2007.igem.org/wiki/index.php/BerkiGEM2007-SamanthaConstructionFile8"