Imperial

From 2007.igem.org

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<h2>Foundational technology: Power your iGEM projects with Cell-Free System Chassis!</h2>
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Revision as of 14:16, 19 October 2007

Project Overview

Our contribution to the synthetic biology community this year will be the investigation of Cell-Free Chassis, the common platform on which Cell By Date and Infector Detector will be built.

Our Achievements

  • Investigated and characterised the in-vitro chassis
  • Designed and modelled a device to detect biofilms and potentially prevent diseases like MRSA
  • Characterised Infector Detector in terms of various AHL concentrations, at room temperature
  • Designed and modelled a device to indicate spoilage of meat/break in the cold chain via the thermal history of the system
  • Characterised the pTet-GFP and pT7-GFP construct in-vitro

Main Project - Infector Detector

Infector Detector is a system that will detect the presence of biofilm infections on urinary catheters by reporting on the presence of AHL, a signalling molecule used by E.coli, and output a fluorescent protein as a result. (more...)


 Image for Specs.
SPECIFICATIONS
Image for Testing/Validation.
TESTING / VALIDATION 		Image for full system.
 Image for Design.
DESIGN

Image for full system.
INFECTOR DETECTOR
Image for Implementation.
IMPLEMENTATION
Image for Modelling.
MODELLING

Foundational technology: Power your iGEM projects with Cell-Free System Chassis!

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Sub Project - Cell by Date

Cell by Date will report when beef has been out of the fridge for too long. It produces fluorescent proteins at ambient temperatures above 8°C. (more...)

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