Paris/October 19

From 2007.igem.org

(Difference between revisions)
(→Digestion reactions)
Line 18: Line 18:
| style="background: #ccffcc;" |D22bis
| style="background: #ccffcc;" |D22bis
|pSB1A2 BBv
|pSB1A2 BBv
-
|MP6
+
|MP6.1 et .2
|EcoRI
|EcoRI
|PstI
|PstI
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 +
|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D88
 +
|pTet>>lox71-gfptripqrt FV
 +
|L34.1 et .2
 +
|EcoRI
 +
|XbaI
 +
|
 +
|pTet>>lox71-gfptripqrt pSB1A2 dig. for use as a FV
 +
|
 +
|
 +
|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D89
 +
|T-lox66-rbsdapAcoli BV
 +
|L53.7
 +
|SpeI
 +
|PstI
 +
|
 +
|T-lox66-rbsdapAcoli pSB1A2 dig. for use as a BV
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|
 +
|
 +
|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D90
 +
|T-lox66-rbsdapAsubtilis BV
 +
|L56
 +
|SpeI
 +
|PstI
 +
|
 +
|T-lox66-rbsdapAsubtilis pSB1A2 dig. for use as a BV
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|
 +
|
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|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D91
 +
|ptet>>lox71-gfpT-lox66-mrfp pSB1A2 BV
 +
|L59.6, .7
 +
|SpeI
 +
|PstI
 +
|
 +
|ptet>>lox71-gfpT-lox66-mrfp pSB1A2 dig. for use as a BV
 +
|
 +
|
 +
|
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|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D92
 +
|rbsdapAcoli BI
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|L39.1
 +
|XbaI
 +
|PstI
 +
|
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|rbsdapAcoli extracted for use as a BI
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|
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|
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|
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|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D93
 +
|rbsdapAsubtilis BI
 +
|L64.1, .2
 +
|XbaI
 +
|PstI
 +
|
 +
|rbsdapAsubtilis extracted for use as a BI
 +
|
 +
|
 +
|
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|}
 +
 +
 +
More digestions that need to be performed
 +
 +
 +
{|
 +
|- style="background: #ccccff;"
 +
! colspan="5" style="background: #ccccff;" | Digestion Products
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| Number
 +
|width=24%| Product Name
 +
|width=24%| Matrix Name
 +
|width=5%| Enzyme 1
 +
|width=5%| Enzyme 2
 +
|width=5%| Size
 +
|width=34%| Description
 +
|width=1%|
 +
|width=1%|
 +
|width=1%|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D76
| style="background: #ccffcc;" |D76
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|frt1-CmR-frt2-T1T2 PCR product digested for cloning as a BB
|frt1-CmR-frt2-T1T2 PCR product digested for cloning as a BB
-
|
 
-
|
 
-
|
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D88
 
-
|pTet>>lox71-gfptripqrt FV
 
-
|L34
 
-
|EcoRI
 
-
|XbaI
 
-
|
 
-
|pTet>>lox71-gfptripqrt pSB1A2 dig. for use as a FV
 
-
|
 
-
|
 
-
|
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D89
 
-
|T-lox66-rbsdapAcoli BV
 
-
|L53
 
-
|SpeI
 
-
|PstI
 
-
|
 
-
|T-lox66-rbsdapAcoli pSB1A2 dig. for use as a BV
 
-
|
 
-
|
 
-
|
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D90
 
-
|T-lox66-rbsdapAsubtilis BV
 
-
|L56
 
-
|SpeI
 
-
|PstI
 
-
|
 
-
|T-lox66-rbsdapAsubtilis pSB1A2 dig. for use as a BV
 
-
|
 
-
|
 
-
|
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D91
 
-
|ptet>>lox71-gfpT-lox66-mrfp pSB1A2 BV
 
-
|L59
 
-
|SpeI
 
-
|PstI
 
-
|
 
-
|ptet>>lox71-gfpT-lox66-mrfp pSB1A2 dig. for use as a BV
 
-
|
 
-
|
 
-
|
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D92
 
-
|rbsdapAcoli BI
 
-
|L39
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|rbsdapAcoli extracted for use as a BI
 
-
|
 
-
|
 
-
|
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D93
 
-
|rbsdapAsubtilis BI
 
-
|L64
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|rbsdapAsubtilis extracted for use as a BI
 
|
|
|
|
|
|
|}
|}

Revision as of 15:19, 19 October 2007

Digestion reactions

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description
D22bis pSB1A2 BBv MP6.1 et .2 EcoRI PstI pSB1A2 vector digested with EcoRI and PstI enzymes
D88 pTet>>lox71-gfptripqrt FV L34.1 et .2 EcoRI XbaI pTet>>lox71-gfptripqrt pSB1A2 dig. for use as a FV
D89 T-lox66-rbsdapAcoli BV L53.7 SpeI PstI T-lox66-rbsdapAcoli pSB1A2 dig. for use as a BV
D90 T-lox66-rbsdapAsubtilis BV L56 SpeI PstI T-lox66-rbsdapAsubtilis pSB1A2 dig. for use as a BV
D91 ptet>>lox71-gfpT-lox66-mrfp pSB1A2 BV L59.6, .7 SpeI PstI ptet>>lox71-gfpT-lox66-mrfp pSB1A2 dig. for use as a BV
D92 rbsdapAcoli BI L39.1 XbaI PstI rbsdapAcoli extracted for use as a BI
D93 rbsdapAsubtilis BI L64.1, .2 XbaI PstI rbsdapAsubtilis extracted for use as a BI


More digestions that need to be performed


Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description
D76 DGAT BB P14(19/10/07) EcoRI PstI DGAT (delta PstI)
D79 DGAT BI P14(19/10/07) XbaI PstI DGAT(delta PstI)PCR product for cloming as a BB in D47
D80 PacI[ptet>>lox71-gfpT-lox66-mrfp]SmaI P15 PacI SmaI ptet>>lox71-gfpT-lox66-mrfp P15 PCR product digested by PacI-SmaI for cloning into pY-2C-IntC
D81 PacI[pY-2C-IntC]SmaI pY-2C-IntC plasmid PacI SmaI pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning
D82 frt1-CmR-frt2 FI P16 EcoRI SpeI frt1-CmR-frt2 PCR product digested for use as a FI
D83 frt1-CmR-frt2 BI P16 XbaI PstI frt1-CmR-frt2 PCR product digested for use as a BI
D84 frt1-CmR-frt2 BBi P16 EcoRI PstI frt1-CmR-frt2 PCR product digested for cloning as a BB
D85 frt1-CmR-frt2-T1T2 FI P17 EcoRI SpeI frt1-CmR-frt2-T1T2 PCR product digested for use as a FI
D86 frt1-CmR-frt2-T1T2 BI P17 XbaI- PstI frt1-CmR-frt2-T1T2 PCR product digested for use as a BI
D87 frt1-CmR-frt2-T1T2 BBi P17 EcoRI PstI frt1-CmR-frt2-T1T2 PCR product digested for cloning as a BB
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