Tokyo/Wash
From 2007.igem.org
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== Wash == | == Wash == | ||
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<br>take 200 ml of culture in a Falcon tube into a eppendorf tube (1.6 or 2.0 ml tube) | <br>take 200 ml of culture in a Falcon tube into a eppendorf tube (1.6 or 2.0 ml tube) | ||
<br>centrifugation for 2 min at 9000g | <br>centrifugation for 2 min at 9000g | ||
Revision as of 09:08, 20 October 2007
Wash
take 200 ml of culture in a Falcon tube into a eppendorf tube (1.6 or 2.0 ml tube)
centrifugation for 2 min at 9000g
discard the supernatant with a pippet
add 200 ml of PBS and dissolve the pellet
centrifugation for 2 min at 9000g
discard the supernatant with a pippet
add 200 ml of PBS and dissolve the pellet
mix well
apply 150 ml onto a well
