BerkiGEM2007-SamanthaConstructionFile8
From 2007.igem.org
(Difference between revisions)
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| - | '''[lox][term][lox]''' <br> | + | '''[rbs][ATG][lox][term][lox]''' <br> |
'''2 Different terminators (1124 and 1092 - will probably use 1092 in the end)''' <br> | '''2 Different terminators (1124 and 1092 - will probably use 1092 in the end)''' <br> | ||
| + | '''using Bca1090 rbs site''' <br> | ||
---- | ---- | ||
| - | Digest pBca9145- | + | Digest pBca9145-I716204 (BamHI/AlwnI, 1547+553bp, Large) <br> |
| - | Digest pBca9145- | + | Digest pBca9145-I716207 (BglII/AlwnI, 1519+797bp, Small) <br> |
| - | Product is pBca9145- | + | Product is pBca9145-I716214. <br> |
| - | Digest pBca9145- | + | Digest pBca9145-I716204 (BamHI/AlwnI, 1547+553bp, Large) <br> |
| - | Digest pBca9145- | + | Digest pBca9145-I716208 (BglII/AlwnI, 1519+768bp, Small) <br> |
| - | Product is pBca9145- | + | Product is pBca9145-I716215. <br> |
Latest revision as of 18:06, 8 June 2007
[rbs][ATG][lox][term][lox]
2 Different terminators (1124 and 1092 - will probably use 1092 in the end)
using Bca1090 rbs site
Digest pBca9145-I716204 (BamHI/AlwnI, 1547+553bp, Large)
Digest pBca9145-I716207 (BglII/AlwnI, 1519+797bp, Small)
Product is pBca9145-I716214.
Digest pBca9145-I716204 (BamHI/AlwnI, 1547+553bp, Large)
Digest pBca9145-I716208 (BglII/AlwnI, 1519+768bp, Small)
Product is pBca9145-I716215.
