USTC/Logic-Gate Promoters
From 2007.igem.org
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= Repression Assay = | = Repression Assay = | ||
== Build Up Promoter Family == | == Build Up Promoter Family == | ||
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| + | Firstly, we extend both sides of the conservative region for transcriptional initiation of PlacUV5[], including -35 box，-10 box and +1 starting point, with two non-sense sequence selected from random groups. The product is named as P_template1 as it is the template for the promoter family. These two non-sense sequence have three main characters: | ||
| + | # They will never include the restriction enzyme cutting sites that will be involved in the whole study; | ||
| + | # They will never include the recognition sites of RNA Polymerases and those of either of the two repressors; | ||
| + | # They will never present in complicated structures. | ||
| + | |||
| + | Secondly, another group of primers, of which the elongation region at 5’ end may contain a unique operator sequence or each, is applied at both ends of P_template1, equipping us with an according group of promoters with complete structures. These promoters can include variant operator sequences at different position in flank of the conservative region. | ||
== Solo-Repression Assay == | == Solo-Repression Assay == | ||
Revision as of 16:20, 21 October 2007
Contents |
Cis-acting Bio-Logic Gates
Model
Schemes of Bio-Logic Promoters
Repression Assay
Build Up Promoter Family
Firstly, we extend both sides of the conservative region for transcriptional initiation of PlacUV5[], including -35 box，-10 box and +1 starting point, with two non-sense sequence selected from random groups. The product is named as P_template1 as it is the template for the promoter family. These two non-sense sequence have three main characters:
- They will never include the restriction enzyme cutting sites that will be involved in the whole study;
- They will never include the recognition sites of RNA Polymerases and those of either of the two repressors;
- They will never present in complicated structures.
Secondly, another group of primers, of which the elongation region at 5’ end may contain a unique operator sequence or each, is applied at both ends of P_template1, equipping us with an according group of promoters with complete structures. These promoters can include variant operator sequences at different position in flank of the conservative region.
