Tokyo/Preliminary assays

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[[Image:AHL Assay2.JPG|thumb|200px| '''Fig.1: '''Exogenously added AHL]]
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Preliminaly assays were done
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<br>- to practice and experience the assays with AHL and IPTG
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<br>- to check if and how our materials actually worked.
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== [[Tokyo/AHL preparation |AHL preparation]] ==
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== [[Tokyo/Experimental precision and accurecy|Experimental precision and accurecy]] ==
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== [[Tokyo/AHL assay practice|AHL assay practice]] ==
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== [[Tokyo/Lux-lac hybrid promoter activation check|Lux-lac hybrid promoter activation check]] ==
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== [[Tokyo/OD correction|OD correction]] ==
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== [[Tokyo/Wash|Wash]] ==
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== [[Tokyo/New AHL check|New AHL check]] ==
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== [[Tokyo/Hybrid promoter activation & repression check|Hybrid promoter activation & repression check ]] ==
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<br>

Revision as of 11:09, 22 October 2007

Fig.1: Exogenously added AHL

Preliminaly assays were done
- to practice and experience the assays with AHL and IPTG
- to check if and how our materials actually worked.

Contents

AHL preparation

Experimental precision and accurecy

AHL assay practice

Lux-lac hybrid promoter activation check

OD correction

Wash

New AHL check

Hybrid promoter activation & repression check