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We made a bacterial society that follows Pareto's principle as an Ant society does.
Bistability and coexistence by cell-cell communication.
Genetic circuit construction
new parts
We indicated our goal of the project by the simulation!! The simulation proved that our genetic circuit have existence of solution.
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We need to develop the promoter that can receive two input, AHL & reperssor. We tried to create and succeeded!!
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We wrote the Formulation
We decided the parameter of hill coefficients by the assay of AHL & IPTG
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wet実験で求められたパラメータを用いて、集団が怠け者の細胞のみとなった場合に、一度不安定化して、続いて働き者と怠け者になれることをStocastic Simulationで確認した。このシステムの挙動に必要な他のパラメータ範囲が判明した。
Based on stochastic simulation using parameters obtained from our wet experiments, we have confirmed that the whole system becomes unstable when there are only idlers left, and then, they become either of workers and idlers. Also we have determined other parameters necessary for the desired behavior of this system.
Simulationを満たすための他のパラメータが現在のプラスミドで可能であるかを、大腸菌を用いた実験で確認した。
1. By the wet experiments using E. coli, we testified whether our current plasmids could satisfy the other required conditions (for the desired behavior of the system) estimated by the simulation.
2. We tested whether our plasmids gave the other parameters to satisfy the simulation by the experiments using E. coli.
5.Future works
Based on the results from the wet experiments in the step 4, the next dry approach - analysis and simulation - should be performed, which will in turn be testified and confirmed by wet experiments.
Continueing these processes, if time permits, would further sophisticate our model.
Students
Satoru Akama | Kenichiro Iwasaki | Hiroki Kawahara | Hajime Nakatani
Advisors: Last year's member
Shotaro Ayukawa | Akio Kobayashi
Instructors
[http://www.sb.dis.titech.ac.jp/ Daisuke Kiga] | [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura] | [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya] | [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]
Links
sunaba
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