Talk:Tokyo Tech

From 2007.igem.org

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===4.<span style="color:#ff33cc;">Wet </span>: 　[[Tokyo/Assay |Assay]]===
===4.<span style="color:#ff33cc;">Wet </span>: 　[[Tokyo/Assay |Assay]]===
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<!--====We determined the feasibility test of this ====-->
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We tested whether our plasmids gave the other parameters to satisfy the simulation by the experiments using ''E. coli''.
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Simulationを満たすための他のパラメータが現在のプラスミドで可能であるかを、大腸菌を用いた実験で確認した。
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1. By the wet experiments using ''E. coli'', we testified whether our current plasmids could satisfy the other required conditions (for the desired behavior of the system) estimated by the simulation.
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2. We tested whether our plasmids gave the other parameters to satisfy the simulation by the experiments using ''E. coli''.
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===5.Future works===
===5.Future works===

Revision as of 13:42, 22 October 2007

how about arranging this page like this? --Hiroki 22:33, 16 October 2007 (EDT)

Homepage design.jpg


Previous homepage design--Hiroki 12:25, 17 October 2007 (EDT) Tokyo alliance header.JPG


Project

Concept

Works

Parts

Wet

Dry


Information

Team

Members

Advisors

Instructors

  • [http://www.sb.dis.titech.ac.jp/ Daisuke Kiga]
  • [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura]
  • [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya]
  • [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]

Links

Cycle.jpg


Tokyotech3.JPG

1. Abstruct

Concept & Model

We made a bacterial society that follows Pareto's principle as an Ant society does.
TOP.JPG

Requirements

Bistability and coexistence by cell-cell communication.

Genetic circuit Tokyo genetic circuit s.jpg

Genetic circuit construction
new parts

GOAL

We indicated our goal of the project by the simulation!! The simulation proved that our genetic circuit have existence of solution.
Tokyo simulation s2.jpg
Our goal.JPG

2. Works

Combinational Wet & Dry Approaches

0. Wet :　Hybrid promoter

Successful development of a new and necessary promoter regulated by two inputs!!

Hybridgraph.JPG

1.Dry :　 Formulation

We wrote the Formulation

2.Wet : 　Assay

We decided the parameter of hill coefficients by the assay of AHL & IPTG

Hill AHL.JPG|Hill IPTG.JPG

3.Dry : 　Simulation


Tokyo simulation s2.jpg

4.Wet : 　Assay

We tested whether our plasmids gave the other parameters to satisfy the simulation by the experiments using E. coli.

5.Future works

Based on the results from the wet experiments in the step 4, the next dry approach - analysis and simulation - should be performed, which will in turn be testified and confirmed by wet experiments. Continueing these processes, if time permits, would further sophisticate our model.


3,About our team TeamLogo.jpg


Students
Satoru Akama | Kenichiro Iwasaki | Hiroki Kawahara | Hajime Nakatani

Advisors: Last year's member
Shotaro Ayukawa | Akio Kobayashi

Instructors
[http://www.sb.dis.titech.ac.jp/ Daisuke Kiga] | [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura] | [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya] | [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]

Links sunaba

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