Week 17

From 2007.igem.org

Revision as of 10:19, 23 October 2007

10/22/07

• Ligation:

-[http://partsregistry.org/Part:BBa_I763032 I763032] (on [http://partsregistry.org/Part:pSB4A3 pSB4A3]);

• Transformation:

-[http://partsregistry.org/Part:BBa_I763032 I763032] (on [http://partsregistry.org/Part:pSB4A3 pSB4A3]);

• We inoculate:

-[http://partsregistry.org/Part:BBa_I763040 I763040] (on high copy plasmid);

-[http://partsregistry.org/Part:BBa_I763031 I763031] (on low copy plasmid), 3 different inocules using 3 different colonies;

-[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (high copy plasmid).

-[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (low copy plasmid).

10/23/07

• Miniprep:

-[http://partsregistry.org/Part:BBa_I763040 I763040] (on high copy plasmid);

-[http://partsregistry.org/Part:BBa_I763029 I763029](high copy plasmid).

-[http://partsregistry.org/Part:BBa_I763029 I763029](low copy plasmid).

• Control Digestion for results impagination:

-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763036 I763036];

-[http://partsregistry.org/Part:BBa_I763019 I763019];

-[http://partsregistry.org/Part:BBa_I763032 I763032];

-[http://partsregistry.org/Part:BBa_I763025 I763025] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763027 I763027];

-[http://partsregistry.org/Part:BBa_I763029 I763029] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763029 I763029] (high copy plasmid).

• Inoculate [http://partsregistry.org/Part:BBa_I763032 I763032] (on [http://partsregistry.org/Part:pSB4A3 pSB4A3]).

10/24/07

10/25/07

10/26/07

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