From 2007.igem.org

Revision as of 09:38, 10 September 2007 (view source) Robertina (Talk | contribs) ← Older edit		Latest revision as of 11:36, 23 October 2007 (view source) Diego (Talk | contribs)
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-	Our finally vector will be YCplac22.This is a centromeric plasmid,with trip resistence,it's long 4854pb.	+	Our final vector will be YCplac22,an YC-type (centromeric) shuttle vector.
		+
		+	'''Vector Information''':
		+
		+	'''Vector type''': plasmid
		+
		+	'''Size (kb): ''' 4.854
		+
		+	'''Construct size (kb): ''' 4.854
		+
		+	'''Construction:''' pUC19
		+
		+	'''Markers:''' TRP1, ampR
		+
		+	'''Features:''' insert detection: lacZ'
		+
		+	'''marker(s):''' TRP1, ampR
		+
		+	'''promoter:''' lac
		+
		+	'''replicon:''' ARS1, pMB1
		+
		+	'''MCS:''' HindIII...EcoRI
		+
		+	'''centromere:''' CEN4
		+
	[[Image:YCplac22_map.jpg]]		[[Image:YCplac22_map.jpg]]
		+
		+	'''Selected features'''
		+
		+	lac_promoter                      142 - 171
		+
		+	M13_pUC_rev_primer                185 - 207
		+
		+	M13_reverse_primer                206 - 224
		+
		+	M13_forward20_primer              306 - 290
		+
		+	M13_pUC_fwd_primer                321 - 299
		+
		+	CEN4                              504 - 1356
		+
		+	Orf frame 2                      2740 - 2066
		+
		+	TRP1                              2740 - 2066
		+
		+	AmpR_promoter                    2983 - 3011
		+
		+	Orf frame 2                      3053 - 3913
		+
		+	Ampicillin                        3053 - 3913
		+
		+	pBR322_origin                    4068 - 4687

---

Latest revision as of 11:36, 23 October 2007

Our final vector will be YCplac22,an YC-type (centromeric) shuttle vector.

Vector Information:

Vector type: plasmid

Size (kb): 4.854

Construct size (kb): 4.854

Construction: pUC19

Markers: TRP1, ampR

Features: insert detection: lacZ'

marker(s): TRP1, ampR

promoter: lac

replicon: ARS1, pMB1

MCS: HindIII...EcoRI

centromere: CEN4

Selected features

lac_promoter 142 - 171

M13_pUC_rev_primer 185 - 207

M13_reverse_primer 206 - 224

M13_forward20_primer 306 - 290

M13_pUC_fwd_primer 321 - 299

CEN4 504 - 1356

Orf frame 2 2740 - 2066

TRP1 2740 - 2066

AmpR_promoter 2983 - 3011

Orf frame 2 3053 - 3913

Ampicillin 3053 - 3913

pBR322_origin 4068 - 4687