Primers Design
From 2007.igem.org
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| - | We | + | We designed two oligo primers for 2Xore,1ORE and Luc with our restriction enzyme to amplify from plasmid p2UG-2XOre-Luc: |
[[Image:oligo.jpg]] | [[Image:oligo.jpg]] | ||
2X ORE – CYC1 Hind III ORE Fw | 2X ORE – CYC1 Hind III ORE Fw | ||
| - | 5’-CCC | + | 5’-CCC AAGCTT TAGTCAATAATGGGAGCTGG-3’ |
Tm 62°C | Tm 62°C | ||
2X ORE – CYC1 BamHI Rev | 2X ORE – CYC1 BamHI Rev | ||
| - | 5’-CGC | + | 5’-CGC GGATCC GTGCGTTTGTGGCGCCCTTA-3’ |
Tm 68°C | Tm 68°C | ||
1X ORE – CYC1 Hind III ORE Fw | 1X ORE – CYC1 Hind III ORE Fw | ||
| - | 5’-CCC | + | 5’-CCC AAGCTT GGCTCTACCGAGCTGGTAGA-3’ |
1X ORE – CYC1 BamHI Rev | 1X ORE – CYC1 BamHI Rev | ||
| - | 5’-CGC | + | 5’-CGC GGATCC GTGCGTTTGTGGCGCCCTTA-3’ |
LUCIFERASE BamH1 Fv | LUCIFERASE BamH1 Fv | ||
| - | 5’- CGC | + | 5’- CGC GGATCC ATGGAAGACGCCAAAAACATAA-3’ |
Tm 60°C | Tm 60°C | ||
LUCIFERASE SacI Rv | LUCIFERASE SacI Rv | ||
| - | 5’- CGC | + | 5’- CGC GAGCTC TTAAAGCTTCTTTCCGCCCTT-3’ |
Tm 60°C | Tm 60°C | ||
| + | |||
| + | PHO 80 fw Bam HI | ||
| + | 5’- CGC GGATCC ATGGAAAGCACATCAGGAGA-3’ | ||
| + | Tm 69.5°C | ||
| + | |||
| + | PHO 80 rev Sac I | ||
| + | 5’- CGC GAGCTC TTAATCTGGCTTTGATCGCT-3’ | ||
| + | Tm 68.1°C | ||
Latest revision as of 11:41, 23 October 2007
We designed two oligo primers for 2Xore,1ORE and Luc with our restriction enzyme to amplify from plasmid p2UG-2XOre-Luc:
2X ORE – CYC1 Hind III ORE Fw 5’-CCC AAGCTT TAGTCAATAATGGGAGCTGG-3’ Tm 62°C
2X ORE – CYC1 BamHI Rev 5’-CGC GGATCC GTGCGTTTGTGGCGCCCTTA-3’ Tm 68°C
1X ORE – CYC1 Hind III ORE Fw 5’-CCC AAGCTT GGCTCTACCGAGCTGGTAGA-3’
1X ORE – CYC1 BamHI Rev 5’-CGC GGATCC GTGCGTTTGTGGCGCCCTTA-3’
LUCIFERASE BamH1 Fv 5’- CGC GGATCC ATGGAAGACGCCAAAAACATAA-3’ Tm 60°C
LUCIFERASE SacI Rv 5’- CGC GAGCTC TTAAAGCTTCTTTCCGCCCTT-3’ Tm 60°C
PHO 80 fw Bam HI 5’- CGC GGATCC ATGGAAAGCACATCAGGAGA-3’ Tm 69.5°C
PHO 80 rev Sac I 5’- CGC GAGCTC TTAATCTGGCTTTGATCGCT-3’ Tm 68.1°C
