Freiburg07/Ligation
From 2007.igem.org
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'''Ligation''' | '''Ligation''' | ||
- | 1 µl ligase | + | total volume: 10 µl<br> |
- | 1 µl ligase buffer | + | |
- | + | 2 µl vector DNA<br> | |
+ | 6 µl insert DNA<br> | ||
+ | 1 µl ligase<br> | ||
+ | 1 µl ligase buffer (1:10)<br> | ||
+ | |||
+ | |||
+ | |||
+ | [https://2007.igem.org/Freiburg BACK] |
Latest revision as of 12:57, 24 October 2007
Ligation
total volume: 10 µl
2 µl vector DNA
6 µl insert DNA
1 µl ligase
1 µl ligase buffer (1:10)