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-	Transformation of frozen competent cells	+	'''Transformation of frozen chem. competent cells'''
-	1. Thaw frozen cells on ice, 10-15 minutes.	+	1. Thaw frozen cells.<BR>
-	2. Add 80 µl cells to the 20 µl ligation reaction.	+	2. Add 10 µl ligation and shake gently.<BR>
-	3. Incubate on ice for 5 minutes.	+	3. Incubate on ice for 5-10 minutes.<br>
-	4. Heat shock cells for 10 minutes at 37 °C.	+	4. Heat shock cells for 10-20 minutes at 37 °C.<BR>
-	5. Bring up in 1 mL LB and shake gently for 1 hour at 37 °C.	+	5. Add 900 µl DYT-Medium and shake gently for 60-70 minutes at 37 °C.<br>
		+
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Latest revision as of 15:00, 24 October 2007

Transformation of frozen chem. competent cells

1. Thaw frozen cells.

2. Add 10 µl ligation and shake gently.

3. Incubate on ice for 5-10 minutes.

4. Heat shock cells for 10-20 minutes at 37 °C.

5. Add 900 µl DYT-Medium and shake gently for 60-70 minutes at 37 °C.

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