Template:BerkiGEM2007 DavidSequencingFiles
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- | [[David_Tulga_Notebook|David Tulga's Notebook]] | + | '''[[David_Tulga_Notebook|<< Back to David Tulga's Notebook]]'''<br><br> |
+ | |||
+ | '''Standard Primers'''<br> | ||
+ | F = ca998<br> | ||
+ | R = G00101<br> | ||
+ | |||
+ | All Plasmid Numbers are I716... | ||
+ | |||
{| | {| | ||
|- | |- | ||
- | |Sample||Date||Plasmid||Clone||Primer||Result|| | + | |'''Sample'''||'''Date'''||'''Plasmid'''||'''Clone'''||'''Primer'''||'''Result'''||'''Link''' |
|- | |- | ||
- | |DT001|| | + | |DT001||06/14/07||102||1||F||Garbled Sequence||[[BerkiGEM2007-Sequencing-DT001 | DT001]] |
|- | |- | ||
- | |DT002|| | + | |DT002||06/14/07||102||2||F||Garbled Sequence||[[BerkiGEM2007-Sequencing-DT002 | DT002]] |
|- | |- | ||
- | |DT003|| | + | |DT003||06/14/07||104||1||F||Sample Mixup||[[BerkiGEM2007-Sequencing-DT003 | DT003]] |
|- | |- | ||
- | |DT004|| | + | |DT004||06/14/07||104||2||F||Genomic DNA Framgent||[[BerkiGEM2007-Sequencing-DT004 | DT004]] |
|- | |- | ||
- | |DT005|| | + | |DT005||06/14/07||105||1||F||Perfect!||[[BerkiGEM2007-Sequencing-DT005 | DT005]] |
|- | |- | ||
- | |DT006|| | + | |DT006||06/14/07||105||2||F||RFP Parent Recombination A||[[BerkiGEM2007-Sequencing-DT006 | DT006]] |
|- | |- | ||
- | |DT007|| | + | |DT007||06/14/07||106||1||F||Perfect!||[[BerkiGEM2007-Sequencing-DT007 | DT007]] |
|- | |- | ||
- | |DT008|| | + | |DT008||06/14/07||106||2||F||RFP Parent Recombination A||[[BerkiGEM2007-Sequencing-DT008 | DT008]] |
|- | |- | ||
- | |DT009|| | + | |DT009||06/14/07||107||1||F||RFP Parent Recombination A||[[BerkiGEM2007-Sequencing-DT009 | DT009]] |
|- | |- | ||
- | |DT010|| | + | |DT010||06/14/07||107||2||F||Perfect!||[[BerkiGEM2007-Sequencing-DT010 | DT010]] |
|- | |- | ||
- | |DT011|| | + | |DT011||06/15/07||102||1||F||Primer Dimer||[[BerkiGEM2007-Sequencing-DT011 | DT011]] |
|- | |- | ||
- | |DT012|| | + | |DT012||06/15/07||102||2||F||Primer Dimer||[[BerkiGEM2007-Sequencing-DT012 | DT012]] |
|- | |- | ||
- | |DT013|| | + | |DT013||06/15/07||104||1||F||Sample Mixup||[[BerkiGEM2007-Sequencing-DT013 | DT013]] |
|- | |- | ||
- | |DT014|| | + | |DT014||06/15/07||104||2||F||Genomic DNA Framgent||[[BerkiGEM2007-Sequencing-DT014 | DT014]] |
|- | |- | ||
- | |DT015|| | + | |DT015||06/15/07||105||1||F||Perfect!||[[BerkiGEM2007-Sequencing-DT015 | DT015]] |
|- | |- | ||
- | |DT016|| | + | |DT016||06/15/07||106||1||F||Perfect!||[[BerkiGEM2007-Sequencing-DT016 | DT016]] |
|- | |- | ||
- | |DT017|| | + | |DT017||06/15/07||107||2||F||Perfect!||[[BerkiGEM2007-Sequencing-DT017 | DT017]] |
|- | |- | ||
- | |DT018|| | + | |DT018||06/19/07||103||2||F||Genomic DNA Framgent||[[BerkiGEM2007-Sequencing-DT018 | DT018]] |
|- | |- | ||
- | |DT019|| | + | |DT019||06/19/07||103||4||F||Genomic DNA Framgent||[[BerkiGEM2007-Sequencing-DT019 | DT019]] |
|- | |- | ||
- | |DT020|| | + | |DT020||06/26/07||102||1||F||Probably Correct (Read quality at end is low)||[[BerkiGEM2007-Sequencing-DT020 | DT020]] |
|- | |- | ||
- | |DT021|| | + | |DT021||06/26/07||102||2||F||Right Gene - Too many mutations||[[BerkiGEM2007-Sequencing-DT021 | DT021]] |
|- | |- | ||
- | |DT022|| | + | |DT022||06/26/07||102||3||F||Right Gene - Too many mutations||[[BerkiGEM2007-Sequencing-DT022 | DT022]] |
|- | |- | ||
- | |DT023|| | + | |DT023||06/26/07||102||4||F||Probably Correct (Read quality at end is low)||[[BerkiGEM2007-Sequencing-DT023 | DT023]] |
|- | |- | ||
- | |DT024|| | + | |DT024||06/27/07||102||4||R||Correct! (With mutations from template)||[[BerkiGEM2007-Sequencing-DT024 | DT024]] |
|- | |- | ||
- | |DT025|| | + | |DT025||07/02/07||103||2||F||Probably Correct (Gene is much longer than read)||[[BerkiGEM2007-Sequencing-DT025 | DT025]] |
- | | | + | |- |
- | + | | || || || || || || | |
- | + | |- | |
+ | |DT026||07/02/07||103||4||F||Probably Correct (Gene is much longer than read)||[[BerkiGEM2007-Sequencing-DT026 | DT026]] | ||
+ | |- | ||
+ | |DT027||07/02/07||104||2||F||Probably Correct (Gene is much longer than read)||[[BerkiGEM2007-Sequencing-DT027 | DT027]] | ||
+ | |- | ||
+ | |DT028||07/02/07||104||3||F||Probably Correct (Gene is much longer than read)||[[BerkiGEM2007-Sequencing-DT028 | DT028]] | ||
+ | |- | ||
+ | |DT029||07/03/07||103||2||R||Probably Correct (Gene is much longer than read)||[[BerkiGEM2007-Sequencing-DT029 | DT029]] | ||
+ | |- | ||
+ | |DT030||07/03/07||103||4||R||Probably Correct (Gene is much longer than read)||[[BerkiGEM2007-Sequencing-DT030 | DT030]] | ||
+ | |- | ||
+ | |DT031||07/03/07||104||2||R||Probably Correct (Gene is much longer than read)||[[BerkiGEM2007-Sequencing-DT031 | DT031]] | ||
+ | |- | ||
+ | |DT032||07/03/07||104||3||R||Probably Correct (Gene is much longer than read)||[[BerkiGEM2007-Sequencing-DT032 | DT032]] | ||
+ | |- | ||
+ | |DT033||07/10/07||105||M-1||F||Perfect!||[[BerkiGEM2007-Sequencing-DT033 | DT033]] | ||
+ | |- | ||
+ | |DT034||07/10/07||105||M-2||F||Perfect!||[[BerkiGEM2007-Sequencing-DT034 | DT034]] | ||
+ | |- | ||
+ | |DT035||07/13/07||103||3||F||Probably Correct (Gene is much longer than read)||[[BerkiGEM2007-Sequencing-DT035 | DT035]] | ||
+ | |- | ||
+ | |DT036||07/13/07||103||4||F||Correct, except for 1 mutation||[[BerkiGEM2007-Sequencing-DT036 | DT036]] | ||
+ | |- | ||
+ | |DT037||07/13/07||104||1||F||Probably Correct (Gene is much longer than read)||[[BerkiGEM2007-Sequencing-DT037 | DT037]] | ||
+ | |- | ||
+ | |DT038||07/13/07||104||2||F||Probably Correct (Gene is much longer than read)||[[BerkiGEM2007-Sequencing-DT038 | DT038]] | ||
+ | |- | ||
+ | |DT039||07/16/07||103||3||R||Probably Correct (Gene is much longer than read)||[[BerkiGEM2007-Sequencing-DT039 | DT039]] | ||
|- | |- | ||
- | | | + | |DT040||07/16/07||103||4||R||Probably Correct (Gene is much longer than read)||[[BerkiGEM2007-Sequencing-DT040 | DT040]] |
|- | |- | ||
- | | | + | |DT041||07/16/07||104||1||R||Probably Correct (Gene is much longer than read)||[[BerkiGEM2007-Sequencing-DT041 | DT041]] |
|- | |- | ||
- | | | + | |DT042||07/16/07||104||2||R||Probably Correct (Gene is much longer than read)||[[BerkiGEM2007-Sequencing-DT042 | DT042]] |
|- | |- | ||
- | | | + | |DT043||07/17/07||103||3||dt011-T7-seq1||Probably Correct (Gene is much longer than read)||[[BerkiGEM2007-Sequencing-DT043 | DT043]] |
|- | |- | ||
- | | | + | |DT044||07/17/07||104||1||dt011-T7-seq1||Probably Correct (Gene is much longer than read)||[[BerkiGEM2007-Sequencing-DT044 | DT044]] |
|- | |- | ||
- | | | + | |DT045||07/24/07||103||3||dt012-T7-seq2||Correct, except for 1 mutation||[[BerkiGEM2007-Sequencing-DT045 | DT045]] |
|- | |- | ||
- | | | + | |DT046||07/24/07||104||1||dt012-T7-seq2||Correct!||[[BerkiGEM2007-Sequencing-DT046 | DT046]] |
|- | |- | ||
- | | | + | |DT047||07/24/07||109||1||ca1111F||Correct!||[[BerkiGEM2007-Sequencing-DT047 | DT047]] |
|- | |- | ||
- | | | + | |DT048||07/24/07||109||2||ca1111F||Correct!||[[BerkiGEM2007-Sequencing-DT048 | DT048]] |
|- | |- | ||
- | | | + | |DT049||07/24/07||109||3||ca1111F||Correct!||[[BerkiGEM2007-Sequencing-DT049 | DT049]] |
|- | |- | ||
- | | | + | |DT050||07/24/07||109||4||ca1111F||Correct!||[[BerkiGEM2007-Sequencing-DT050 | DT050]] |
|- | |- | ||
- | | | + | | || || || || || || |
|- | |- | ||
- | | | + | |DT051||07/30/07||115||D4||ca570F||Correct, has 1106H RBS (Used PCR)||[[BerkiGEM2007-Sequencing-DT051 | DT051]] |
|- | |- | ||
- | | | + | |DT052||07/31/07||116(101)||2||F,dt003-pir-IR||Truncation Recombination (only ca998 worked)||[[BerkiGEM2007-Sequencing-DT052 | DT052]] |
|- | |- | ||
- | | | + | |DT053||08/03/07||116||1||F||Truncation Recombination||[[BerkiGEM2007-Sequencing-DT053 | DT053]] |
|- | |- | ||
- | | | + | |DT054||08/03/07||116||2||F||Truncation Recombination||[[BerkiGEM2007-Sequencing-DT054 | DT054]] |
|- | |- | ||
- | | | + | |DT055||08/03/07||116||3||F||Truncation Recombination||[[BerkiGEM2007-Sequencing-DT055 | DT055]] |
|- | |- | ||
- | | | + | |DT056||08/03/07||116||4||F||Truncation Recombination||[[BerkiGEM2007-Sequencing-DT056 | DT056]] |
|- | |- | ||
- | | | + | |DT057||08/06/07||117||1||ca570F||Correct!||[[BerkiGEM2007-Sequencing-DT057 | DT057]] |
|- | |- | ||
- | | | + | |DT058||08/06/07||117||2||ca570F||Correct!||[[BerkiGEM2007-Sequencing-DT058 | DT058]] |
|- | |- | ||
- | | | + | |DT059||08/06/07||117||3||ca570F||Correct!||[[BerkiGEM2007-Sequencing-DT059 | DT059]] |
|- | |- | ||
- | | | + | |DT060||08/06/07||117||4||ca570F||Correct!||[[BerkiGEM2007-Sequencing-DT060 | DT060]] |
|- | |- | ||
- | | | + | |DT061||08/13/07||118||1||R||Correct!||[[BerkiGEM2007-Sequencing-DT061 | DT061]] |
|- | |- | ||
- | | | + | |DT062||08/13/07||118||2||R||Appears Correct, but signal is too low||[[BerkiGEM2007-Sequencing-DT062 | DT062]] |
|- | |- | ||
- | | | + | |DT063||08/18/07||119||1||F||Correct!||[[BerkiGEM2007-Sequencing-DT063 | DT063]] |
|} | |} |
Latest revision as of 23:48, 24 October 2007
<< Back to David Tulga's Notebook
Standard Primers
F = ca998
R = G00101
All Plasmid Numbers are I716...
Sample | Date | Plasmid | Clone | Primer | Result | Link |
DT001 | 06/14/07 | 102 | 1 | F | Garbled Sequence | DT001 |
DT002 | 06/14/07 | 102 | 2 | F | Garbled Sequence | DT002 |
DT003 | 06/14/07 | 104 | 1 | F | Sample Mixup | DT003 |
DT004 | 06/14/07 | 104 | 2 | F | Genomic DNA Framgent | DT004 |
DT005 | 06/14/07 | 105 | 1 | F | Perfect! | DT005 |
DT006 | 06/14/07 | 105 | 2 | F | RFP Parent Recombination A | DT006 |
DT007 | 06/14/07 | 106 | 1 | F | Perfect! | DT007 |
DT008 | 06/14/07 | 106 | 2 | F | RFP Parent Recombination A | DT008 |
DT009 | 06/14/07 | 107 | 1 | F | RFP Parent Recombination A | DT009 |
DT010 | 06/14/07 | 107 | 2 | F | Perfect! | DT010 |
DT011 | 06/15/07 | 102 | 1 | F | Primer Dimer | DT011 |
DT012 | 06/15/07 | 102 | 2 | F | Primer Dimer | DT012 |
DT013 | 06/15/07 | 104 | 1 | F | Sample Mixup | DT013 |
DT014 | 06/15/07 | 104 | 2 | F | Genomic DNA Framgent | DT014 |
DT015 | 06/15/07 | 105 | 1 | F | Perfect! | DT015 |
DT016 | 06/15/07 | 106 | 1 | F | Perfect! | DT016 |
DT017 | 06/15/07 | 107 | 2 | F | Perfect! | DT017 |
DT018 | 06/19/07 | 103 | 2 | F | Genomic DNA Framgent | DT018 |
DT019 | 06/19/07 | 103 | 4 | F | Genomic DNA Framgent | DT019 |
DT020 | 06/26/07 | 102 | 1 | F | Probably Correct (Read quality at end is low) | DT020 |
DT021 | 06/26/07 | 102 | 2 | F | Right Gene - Too many mutations | DT021 |
DT022 | 06/26/07 | 102 | 3 | F | Right Gene - Too many mutations | DT022 |
DT023 | 06/26/07 | 102 | 4 | F | Probably Correct (Read quality at end is low) | DT023 |
DT024 | 06/27/07 | 102 | 4 | R | Correct! (With mutations from template) | DT024 |
DT025 | 07/02/07 | 103 | 2 | F | Probably Correct (Gene is much longer than read) | DT025 |
DT026 | 07/02/07 | 103 | 4 | F | Probably Correct (Gene is much longer than read) | DT026 |
DT027 | 07/02/07 | 104 | 2 | F | Probably Correct (Gene is much longer than read) | DT027 |
DT028 | 07/02/07 | 104 | 3 | F | Probably Correct (Gene is much longer than read) | DT028 |
DT029 | 07/03/07 | 103 | 2 | R | Probably Correct (Gene is much longer than read) | DT029 |
DT030 | 07/03/07 | 103 | 4 | R | Probably Correct (Gene is much longer than read) | DT030 |
DT031 | 07/03/07 | 104 | 2 | R | Probably Correct (Gene is much longer than read) | DT031 |
DT032 | 07/03/07 | 104 | 3 | R | Probably Correct (Gene is much longer than read) | DT032 |
DT033 | 07/10/07 | 105 | M-1 | F | Perfect! | DT033 |
DT034 | 07/10/07 | 105 | M-2 | F | Perfect! | DT034 |
DT035 | 07/13/07 | 103 | 3 | F | Probably Correct (Gene is much longer than read) | DT035 |
DT036 | 07/13/07 | 103 | 4 | F | Correct, except for 1 mutation | DT036 |
DT037 | 07/13/07 | 104 | 1 | F | Probably Correct (Gene is much longer than read) | DT037 |
DT038 | 07/13/07 | 104 | 2 | F | Probably Correct (Gene is much longer than read) | DT038 |
DT039 | 07/16/07 | 103 | 3 | R | Probably Correct (Gene is much longer than read) | DT039 |
DT040 | 07/16/07 | 103 | 4 | R | Probably Correct (Gene is much longer than read) | DT040 |
DT041 | 07/16/07 | 104 | 1 | R | Probably Correct (Gene is much longer than read) | DT041 |
DT042 | 07/16/07 | 104 | 2 | R | Probably Correct (Gene is much longer than read) | DT042 |
DT043 | 07/17/07 | 103 | 3 | dt011-T7-seq1 | Probably Correct (Gene is much longer than read) | DT043 |
DT044 | 07/17/07 | 104 | 1 | dt011-T7-seq1 | Probably Correct (Gene is much longer than read) | DT044 |
DT045 | 07/24/07 | 103 | 3 | dt012-T7-seq2 | Correct, except for 1 mutation | DT045 |
DT046 | 07/24/07 | 104 | 1 | dt012-T7-seq2 | Correct! | DT046 |
DT047 | 07/24/07 | 109 | 1 | ca1111F | Correct! | DT047 |
DT048 | 07/24/07 | 109 | 2 | ca1111F | Correct! | DT048 |
DT049 | 07/24/07 | 109 | 3 | ca1111F | Correct! | DT049 |
DT050 | 07/24/07 | 109 | 4 | ca1111F | Correct! | DT050 |
DT051 | 07/30/07 | 115 | D4 | ca570F | Correct, has 1106H RBS (Used PCR) | DT051 |
DT052 | 07/31/07 | 116(101) | 2 | F,dt003-pir-IR | Truncation Recombination (only ca998 worked) | DT052 |
DT053 | 08/03/07 | 116 | 1 | F | Truncation Recombination | DT053 |
DT054 | 08/03/07 | 116 | 2 | F | Truncation Recombination | DT054 |
DT055 | 08/03/07 | 116 | 3 | F | Truncation Recombination | DT055 |
DT056 | 08/03/07 | 116 | 4 | F | Truncation Recombination | DT056 |
DT057 | 08/06/07 | 117 | 1 | ca570F | Correct! | DT057 |
DT058 | 08/06/07 | 117 | 2 | ca570F | Correct! | DT058 |
DT059 | 08/06/07 | 117 | 3 | ca570F | Correct! | DT059 |
DT060 | 08/06/07 | 117 | 4 | ca570F | Correct! | DT060 |
DT061 | 08/13/07 | 118 | 1 | R | Correct! | DT061 |
DT062 | 08/13/07 | 118 | 2 | R | Appears Correct, but signal is too low | DT062 |
DT063 | 08/18/07 | 119 | 1 | F | Correct! | DT063 |