Melbourne/Parts created

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{| border="1"
|+ Parts Created by Melbourne [[Melbourne/parts created graphical| <GRAPHICAL>]]
|+ Parts Created by Melbourne [[Melbourne/parts created graphical| <GRAPHICAL>]]
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! Label !! Part Name !! Part Size(bp) !! Plasmid Vector !! Plasmid Size(bp) !! Total size(bp) !! Resistance  !! Status !! Sequenced
+
! Part Name !! Sub Parts !! Part Size(bp) !! Plasmid Vector !! Plasmid Size(bp) !! Total size(bp) !! Resistance  !! Status !! Sequenced
|-
|-
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! [[Melbourne/OMP-EYFP|P1 1P-P1 11A]]
+
! Gas Vesicle polycistronic ______gene______ [http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
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| OmpR-EYFP (RBS+ LVA term) || 108+878 || pSB1A2 || 2079 || 3065 || Amp || Glycerol stocks in -80 || yes
+
| - || 6064 || BBa_J61035 || 3551 || 9129 || Amp,Gen || Glycerol stocks in -80 || yes
|-
|-
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! [[Melbourne/OmpR-RBS-LacZa-double terminator|P115-P13O-P29E-P11G]]
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! OmpR-EYFP
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| OmpR-RBS-LacZa-double terminator [[Melbourne/BBa_R0082|P1 15P]]-[[Melbourne/BBa_B0034|P1 3O]]-[[Melbourne/BBa_E0033|P2 9E]]-[[Melbourne/BBa_B0014|P1 1G]]|| 108+12+353+95 || pSB1A2 || 2079 || 2657 || Amp || Glycerol stocks in -80 || yes
+
| [http://partsregistry.org/Part:BBa_R0082 OmpR] - [http://partsregistry.org/Part:BBa_E0430 EYFP]|| 108+878 || pSB1A2 || 2079 || 3065 || Amp || Glycerol stocks in -80 || yes
-
! [[Melbourne/OmpR-c1 inverter|P115-P213K]]
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|-
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| OmpR-c1 inverter [[Melbourne/BBa_R0082|P1 15P]]-[[Melbourne/BBa_Q04510|P2 13K]]|| 108+987 || pSB1A2 || 2079 || 3068 || Amp || Glycerol stocks in -80 || yes
+
! LacZ reporter
 +
|  [http://partsregistry.org/Part:BBa_B0034 RBS] - [http://partsregistry.org/Part:BBa_E0033 LacZ alpha] - [http://partsregistry.org/Part:BBa_B0014 double terminator] || 12+353+95 || pSB1A2 || 2079 || 2549 || Amp || Glycerol stocks in -80 || yes
 +
|-
 +
! OmpR-LacZ reporter
 +
|  [http://partsregistry.org/Part:BBa_R0082 OmpR] - [http://partsregistry.org/Part:BBa_B0034 RBS] - [http://partsregistry.org/Part:BBa_E0033 LacZ alpha] - [http://partsregistry.org/Part:BBa_B0014 double terminator] || 108+12+353+95 || pSB1A2 || 2079 || 2657 || Amp || Glycerol stocks in -80 || yes
 +
|-
 +
! OmpR-c1 inverter
 +
|  [http://partsregistry.org/Part:BBa_R0082 OmpR] - [http://partsregistry.org/Part:BBa_Q04510 c1 inverter] || 108+987 || pSB1A2 || 2079 || 3068 || Amp || Glycerol stocks in -80 || yes
 +
|-
 +
! ComPalt
 +
| [[Melbourne/Lab BL Notebook/ComP'|ComP']]||2406 ||pSB1A3||2157||4563||Amp|| Glycerol stocks in -80 || no
 +
|-
 +
! ____ComAalt____  [http://partsregistry.org/Part:BBa_I750013 BBa_I750013]
 +
| [[Melbourne/Lab BL Notebook/ComA'|ComA']] ||741 ||pSB1A3 ||2157 ||2898||Amp|| Glycerol stocks in -80 || no
 +
|-
 +
! GenRBS+ComPalt+ter [http://partsregistry.org/Part:BBa_I750012 BBa_I750012]
 +
| Gen[http://partsregistry.org/Part:BBa_B0034 RBS],[[Melbourne/Lab BL Notebook/ComP'|ComP']], [http://partsregistry.org/Part:BBa_B0014 double terminator]||2468 +1500Gen || pSB1AK3 ||3189||7.1kb||Amp, Kan, Gen|| Miniprepped || no
 +
|-
 +
! GenRBS+ComAalt+ter [http://partsregistry.org/Part:BBa_I750014 BBa_I750014]
 +
| Gen[http://partsregistry.org/Part:BBa_B0034 RBS], [[Melbourne/Lab BL Notebook/ComA'|ComA']], [http://partsregistry.org/Part:BBa_B0014 double terminator]||803 +1500Gen ||pSB1AK3 ||3189|| 5.5kb ||Amp, Kan, Gen|| Miniprepped || no
|-
|-
|}
|}

Latest revision as of 04:54, 25 October 2007

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Last Updated:

Parts Created by Melbourne <GRAPHICAL>
Part Name Sub Parts Part Size(bp) Plasmid Vector Plasmid Size(bp) Total size(bp) Resistance Status Sequenced
Gas Vesicle polycistronic ______gene______ [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] - 6064 BBa_J61035 3551 9129 Amp,Gen Glycerol stocks in -80 yes
OmpR-EYFP [http://partsregistry.org/Part:BBa_R0082 OmpR] - [http://partsregistry.org/Part:BBa_E0430 EYFP] 108+878 pSB1A2 2079 3065 Amp Glycerol stocks in -80 yes
LacZ reporter [http://partsregistry.org/Part:BBa_B0034 RBS] - [http://partsregistry.org/Part:BBa_E0033 LacZ alpha] - [http://partsregistry.org/Part:BBa_B0014 double terminator] 12+353+95 pSB1A2 2079 2549 Amp Glycerol stocks in -80 yes
OmpR-LacZ reporter [http://partsregistry.org/Part:BBa_R0082 OmpR] - [http://partsregistry.org/Part:BBa_B0034 RBS] - [http://partsregistry.org/Part:BBa_E0033 LacZ alpha] - [http://partsregistry.org/Part:BBa_B0014 double terminator] 108+12+353+95 pSB1A2 2079 2657 Amp Glycerol stocks in -80 yes
OmpR-c1 inverter [http://partsregistry.org/Part:BBa_R0082 OmpR] - [http://partsregistry.org/Part:BBa_Q04510 c1 inverter] 108+987 pSB1A2 2079 3068 Amp Glycerol stocks in -80 yes
ComPalt ComP'2406 pSB1A321574563Amp Glycerol stocks in -80 no
____ComAalt____ [http://partsregistry.org/Part:BBa_I750013 BBa_I750013] ComA' 741 pSB1A3 2157 2898Amp Glycerol stocks in -80 no
GenRBS+ComPalt+ter [http://partsregistry.org/Part:BBa_I750012 BBa_I750012] Gen[http://partsregistry.org/Part:BBa_B0034 RBS],ComP', [http://partsregistry.org/Part:BBa_B0014 double terminator]2468 +1500Gen pSB1AK3 31897.1kbAmp, Kan, Gen Miniprepped no
GenRBS+ComAalt+ter [http://partsregistry.org/Part:BBa_I750014 BBa_I750014] Gen[http://partsregistry.org/Part:BBa_B0034 RBS], ComA', [http://partsregistry.org/Part:BBa_B0014 double terminator]803 +1500Gen pSB1AK3 3189 5.5kb Amp, Kan, Gen Miniprepped no
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