Bologna University/Plates preparation
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::1. Autoclave LB medium with 2% agar. | ::1. Autoclave LB medium with 2% agar. | ||
::2. Cool at 50 °C to prevent agar polymerization. | ::2. Cool at 50 °C to prevent agar polymerization. | ||
| - | ::3. Before preparing plates add antibiotic (Ampicillin | + | ::3. Before preparing plates add antibiotic (Ampicillin 1000x [ ] or Kanamicin 200x [ ]). |
| - | ::4. Put about | + | ::4. Put about 20ml of medium per plate. |
::5. Leave it solidify and store at 4°C. | ::5. Leave it solidify and store at 4°C. | ||
[[Bologna University/Protocols | Back]] | [[Bologna University/Protocols | Back]] | ||
Latest revision as of 09:45, 25 October 2007
- 1. Autoclave LB medium with 2% agar.
- 2. Cool at 50 °C to prevent agar polymerization.
- 3. Before preparing plates add antibiotic (Ampicillin 1000x [ ] or Kanamicin 200x [ ]).
- 4. Put about 20ml of medium per plate.
- 5. Leave it solidify and store at 4°C.
