Tokyo/New AHL check
From 2007.igem.org
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<br>The final concantrations of AHL applied here were 0.01, 0.1, 0.5, 1, 10, 100, and 1000 nM. | <br>The final concantrations of AHL applied here were 0.01, 0.1, 0.5, 1, 10, 100, and 1000 nM. | ||
===Result & Conclusion: === | ===Result & Conclusion: === | ||
| - | The ability of the new AHL to activate the | + | The ability of the new AHL to activate the pomoters was almost the twice of that of old one. |
== == | == == | ||
[[Tokyo/Preliminary assays |"Back to Preliminary assays"]] | [[Tokyo/Preliminary assays |"Back to Preliminary assays"]] | ||
Revision as of 11:48, 25 October 2007
Contents |
Purpose:
To determine and compare the activity of the AHL used so far and that of the newly perchased AHL.
Samples:
A4 placQI in pTrc99A (pos. con.)
A4ΔP in pTrc99A (neg. con.)
A4 hybrid promoter in pBR322
Procedure:
The old and the newly delivered AHL was used.
AHL assay Standard protocol
The final concantrations of AHL applied here were 0.01, 0.1, 0.5, 1, 10, 100, and 1000 nM.
Result & Conclusion:
The ability of the new AHL to activate the pomoters was almost the twice of that of old one.
