Berkeley LBL/MimiSequence

From 2007.igem.org

(Difference between revisions)
Line 24: Line 24:
''S-chlD:''
''S-chlD:''
-
           '''S-chlD3-NotI-bglII'''
+
           '''S-chlD3-NotI-bglII-long'''
-
           5' - TTA AAG ATC TTT TGC GGC CGC TTT ATT CTA TTG CAT GTC GGC GAT CGC CTG GCG GGC C - 3'
+
           5' - TTG AAG CCT AGG AGA AGA GTA GGG AAT TTT TTG ACC AAT TTC TAG - 3'
-
           TM (50mM NaCL): 72.5 °C  
+
           TM (50mM NaCL): 63.1 °C  
-
           GC Content: 53.4%  
+
           GC Content: 37.8%  
-
           '''S-chlD5-speI-rbs'''
+
           '''S-chlD5-speI-rbs-long'''
-
           5' - TAT AAC TAG TAA GAA GGA GAT ATA CTT ATG ACC ACC CTG ACC CCC TTT ATT CCC CTC - 3'
+
           5' - ATT GGG AGT CAA CTT CTA CTA AAT CTT CAG AAA AAC GAT ATT TGG - 3'
-
           TM (50mM NaCL): 66 °C  
+
           TM (50mM NaCL): 61.6 °C  
-
           GC Content: 42.1%
+
           GC Content: 33.3%

Revision as of 04:35, 26 October 2007

Sequences and Properties of Oligonucleotides:

S-chlH: 

         S-chlH3-kpnI-BamHI
         5' - TAA AGG ATC CAA AGG TAC CTT ATT CAA CCC CTT CAA TGC GGT CTT CC - 3'
         TM (50mM NaCL): 66.7 °C 
         GC Content: 44.7% 

         S-chlH5-ndeI  
         5' - AAT TCA TAT GAC TGC CAC CCT TGC CGC CCC CAG C - 3'
         TM (50mM NaCL): 70 °C 
         GC Content: 58.8%

S-chlI: 

         S-chlI3-speI-NotI-bglII  
         5' - TAT TAG ATC TTT AGC GGC CGC TTT ACT AGT TTA AGC TTC ATC GAC AAC GCC AAA AAC CCG - 3'
         TM (50mM NaCL): 68.4 °C 
         GC Content: 43.3% 

         S-chlI5-kpnI-rbs  
         5' - TTT AGG TAC CAA GAA GGA GAT ATA CTT ATG ACT GCC ACC CTT GCC GCC CCC AGC - 3'
         Properties TM (50mM NaCL): 70.5 °C 
         GC Content: 51.9%

S-chlD: 

         S-chlD3-NotI-bglII-long
         5' - TTG AAG CCT AGG AGA AGA GTA GGG AAT TTT TTG ACC AAT TTC TAG - 3'
         TM (50mM NaCL): 63.1 °C 
         GC Content: 37.8% 

         S-chlD5-speI-rbs-long
         5' - ATT GGG AGT CAA CTT CTA CTA AAT CTT CAG AAA AAC GAT ATT TGG - 3'
         TM (50mM NaCL): 61.6 °C 
         GC Content: 33.3%
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