Tianjin/DIODE
From 2007.igem.org
Sunlovedie (Talk | contribs) (→Model) |
Sunlovedie (Talk | contribs) (→Model) |
||
Line 23: | Line 23: | ||
We make a mathematical model to simulate the behavior of the system.The model is base on ODEs.With the help of this model,we shows the ideal performance of each immobilized cells ,especially the amplifier cell, under different cell number and flux conditions.We also simulate the whole system at positive and negative and find out the difference of each cell of at positive and negative as well as the situation the diode was broken down.<br> | We make a mathematical model to simulate the behavior of the system.The model is base on ODEs.With the help of this model,we shows the ideal performance of each immobilized cells ,especially the amplifier cell, under different cell number and flux conditions.We also simulate the whole system at positive and negative and find out the difference of each cell of at positive and negative as well as the situation the diode was broken down.<br> | ||
The model can help us with design and experiment. | The model can help us with design and experiment. | ||
+ | <br> | ||
<font size="3" color="#0000CC" bgcolor="#FF9933">1. </font>[[Tianjin/DIODE/Model0|<font size="3" color="#0000CC">Model design and construction</font>]] | <font size="3" color="#0000CC" bgcolor="#FF9933">1. </font>[[Tianjin/DIODE/Model0|<font size="3" color="#0000CC">Model design and construction</font>]] | ||
Revision as of 07:18, 26 October 2007
DesignBrief Introduction of Bio-diode
The diode, one of the most significant parts in the electric integrate circuit and electronic equipment, functions as allowing the current get through when the diode is positive-biased, and cut off while it is negative-biased. When the negative voltage increases to a threshold, the diode tends to be negatively broken down. In this project, the molecular signal AHL is considered as the current of electric circuit.Three types of cells: Generator cells、Amplifier cells and Block cells are immobilized into small beads with a diameter of 0.5 millimeter, then different beads are settled into particular parts of our specially designed equipment sequentially and respectively. On the contrary, if the positions of amplifier cells and block cells in the equipment are reversed, the AHL produced by generator cell would be degraded by block cells at the very beginning resulting in no amplification of the signal. Thus, hardly AHL could be detected in the outflow culture, a condition called ‘negative-biased block’. |
ModelWe make a mathematical model to simulate the behavior of the system.The model is base on ODEs.With the help of this model,we shows the ideal performance of each immobilized cells ,especially the amplifier cell, under different cell number and flux conditions.We also simulate the whole system at positive and negative and find out the difference of each cell of at positive and negative as well as the situation the diode was broken down.
2. Model result 2.1 Model of Generator Cells |
Experiment1.Datas and Graphs of Detector cells
|