Tianjin/DIODE/Experiment2

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Revision as of 08:20, 26 October 2007

Introduction

Equipment

Test Result

Figure 1: We test the equipment containing the generator cell and the block cell at different flux and different time after the flux was changed.The result shows that the output concentration of AHL will go down when the flux increased and the block cell will degradate the AHL produced by the generator cell but still a bit higher than we expected.



Figure 2:We put in more block cells than that in figure 1,that is 1ml immobilized generator cells and 4ml immobilized block cells .The result shows that the block cells also work but not as good as we want.



Figure 3:We test the postive-biased and the negative-biased condition of the system .The result show that the block cell cause the concentration of AHL get down.We can observe that the fluorescence value of postive-biased and negative-biased condition are almost the same which reveals that the amplifier cells are opened at both condition .When we add IPTG,the fluorescence of postive-biased will reach a hihi valuev as we expected.



Figure 4：We test generator cells together with the amplifier cells at flux=55.6ul/s .The result show that the concentration of AHL get higher when amplifier cells was added and the IPTG was added.