Melbourne/Lab Notebook

From 2007.igem.org

< Melbourne(Difference between revisions)
(→Transformation)
 
(84 intermediate revisions not shown)
Line 1: Line 1:
[[Melbourne|<Back to team home page>]]  
[[Melbourne|<Back to team home page>]]  
-
==Week 1==
+
*Gas Vesicle lab notebook:
-
===25 June 2007===
+
** [[Melbourne/Lab GV Notebook|Gas Vesicles Notebook]]
-
Prepared LB agar plates.
+
*Blue photoreceptor notebook:
 +
** [[Melbourne/Lab BL Notebook|Blue Photoreceptor Notebook]]
-
====Transformation====
+
*Photoreceptor & control system lab notebooks:
-
#resuspended the following from Registry plates:
+
** [[Melbourne/Lab Notebook Weeks 1-4|Weeks 1-4]]
-
**BBa_I15008 - ho1 (P2 21A)
+
** [[Melbourne/Lab Notebook Weeks 5-8|Weeks 5-8]]
-
**BBa_I15009 - PcyA (P2 21C)
+
** [[Melbourne/Lab Notebook Weeks 9-12|Weeks 9-12]]
-
**BBa_R0084 - OmpR positive promoter (P1 11H)
+
** [[Melbourne/Lab Notebook Weeks 13-16|Weeks 13-16]]
-
##Punctured foil with pipette tip.
+
** [[Melbourne/Lab Notebook Weeks 17-18|Weeks 17-18]]
-
##Resuspended in 15uL ddH2O.
+
-
##Stored in-20 (after taking 1uL for transformation).
+
-
#[[Melb:Transformation Protocol|Transformed]] into competent DH5alpha cells with shorter incubation times as follows:
+
-
**30min on ice after DNA addition
+
-
**10min on ice after heat shock
+
-
**30min at 37degrees with LB
+
-
 
+
-
===26 June 2007===
+
-
===27 June 2007===
+
-
===28 June 2007===
+
-
===29 June 2007===
+
-
===30 June 2007===
+
-
==Week 2==
+
-
===2 July 2007===
+
-
=Now=
+
-
===3 July 2007===
+
-
===4 July 2007===
+
-
===5 July 2007===
+
-
===6 July 2007===
+
-
==Week 3==
+
-
===9 July 2007===
+
-
===10 July 2007===
+
-
===11 July 2007===
+
-
===12 July 2007===
+
-
===13 July 2007===
+
-
==Week 4==
+
-
===16 July 2007===
+
-
===17 July 2007===
+
-
===18 July 2007===
+
-
===19 July 2007===
+
-
===20 July 2007===
+
-
==Week 5==
+
-
===23 July 2007===
+
-
===24 July 2007===
+
-
===25 July 2007===
+
-
===26 July 2007===
+
-
===27 July 2007===
+
-
==Week 6==
+
-
===30 July 2007===
+
-
===31 July 2007===
+
-
===1  Aug 2007===
+
-
===2  Aug 2007===
+
-
===3  Aug 2007===
+

Latest revision as of 14:25, 26 October 2007

<Back to team home page>

Retrieved from "http://2007.igem.org/wiki/index.php/Melbourne/Lab_Notebook"