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| | [[Melbourne|<Back to team home page>]] | | [[Melbourne|<Back to team home page>]] |
| - | ==Week 1==
| + | *Gas Vesicle lab notebook: |
| - | ===25 June 2007===
| + | ** [[Melbourne/Lab GV Notebook|Gas Vesicles Notebook]] |
| | | | |
| - | Prepared LB agar plates.
| + | *Blue photoreceptor notebook: |
| | + | ** [[Melbourne/Lab BL Notebook|Blue Photoreceptor Notebook]] |
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| - | ====Transformation====
| + | *Photoreceptor & control system lab notebooks: |
| - | #resuspended the following from Registry plates:
| + | ** [[Melbourne/Lab Notebook Weeks 1-4|Weeks 1-4]] |
| - | **BBa_I15008 - ho1 (P2 21A) | + | ** [[Melbourne/Lab Notebook Weeks 5-8|Weeks 5-8]] |
| - | **BBa_I15009 - PcyA (P2 21C) | + | ** [[Melbourne/Lab Notebook Weeks 9-12|Weeks 9-12]] |
| - | **BBa_R0084 - OmpR positive promoter (P1 11H) | + | ** [[Melbourne/Lab Notebook Weeks 13-16|Weeks 13-16]] |
| - | ##Punctured foil with pipette tip.
| + | ** [[Melbourne/Lab Notebook Weeks 17-18|Weeks 17-18]] |
| - | ##Resuspended in 15uL ddH2O.
| + | |
| - | ##Stored in-20 (after taking 1uL for transformation).
| + | |
| - | #[[Melb:Transformation Protocol|Transformed]] into competent DH5alpha cells with shorter incubation times as follows:
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| - | **30min on ice after DNA addition | + | |
| - | **10min on ice after heat shock
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| - | **30min at 37degrees with LB
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| - | | + | |
| - | ===26 June 2007===
| + | |
| - | | + | |
| - | ====Transformation from Monday====
| + | |
| - | *Transformation of BBa_I15008 and BBa_I15009 failed. No colonies on plates
| + | |
| - | *Small number of colonies on BBa_R0084 plate.
| + | |
| - | *Placed in cool room
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| - | | + | |
| - | ====Transformation====
| + | |
| - | #Resuspended the following parts in 15uL:
| + | |
| - | *BBa_B0034 (RBS, plate 1 3O, Amp)
| + | |
| - | *BBa_C0051 (C1 repressor, plate 1 5G, Amp)
| + | |
| - | *BBa_B0010 (Terminator, plate 2 3P, Amp)
| + | |
| - | **Think some DNA may have remained in wells
| + | |
| - | #[[Melb:Transformation Protocol|Transformed]] into competent DH5alpha cells from Joe
| + | |
| - | | + | |
| - | ====Streak plates====
| + | |
| - | #Streaked the following cells:
| + | |
| - | *PJS010 (from solid agar, Amp) | + | |
| - | *Fusion protein (from glycerol stock, Kan) | + | |
| - | *BBa_I15010 (from solid agar, Kan)
| + | |
| - | | + | |
| - | ===27 June 2007===
| + | |
| - | ===28 June 2007===
| + | |
| - | ===29 June 2007===
| + | |
| - | ===30 June 2007===
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| - | ==Week 2==
| + | |
| - | ===2 July 2007===
| + | |
| - | =Now=
| + | |
| - | ===3 July 2007===
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| - | ===4 July 2007===
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| - | ===5 July 2007===
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| - | ===6 July 2007===
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| - | ==Week 3==
| + | |
| - | ===9 July 2007===
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| - | ===10 July 2007===
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| - | ===11 July 2007===
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| - | ===12 July 2007===
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| - | ===13 July 2007===
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| - | ==Week 4==
| + | |
| - | ===16 July 2007===
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| - | ===17 July 2007===
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| - | ===18 July 2007===
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| - | ===19 July 2007===
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| - | ===20 July 2007===
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| - | ==Week 5==
| + | |
| - | ===23 July 2007===
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| - | ===24 July 2007===
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| - | ===25 July 2007===
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| - | ===26 July 2007===
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| - | ===27 July 2007===
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| - | ==Week 6==
| + | |
| - | ===30 July 2007===
| + | |
| - | ===31 July 2007===
| + | |
| - | ===1 Aug 2007===
| + | |
| - | ===2 Aug 2007===
| + | |
| - | ===3 Aug 2007===
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