Imperial/Wet Lab/Lab Notebook/2007-09-10

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__NOTOC__
= 10 September 2007 =
= 10 September 2007 =
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== Vesicles ==
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'''Results''' <br>
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Sample 8, produced on [[Imperial/Wet Lab/Lab Notebook/2007-09-04|04.09.2007]] was looked at again under the fluorescence microscope, but this time only a single 2&mu;m vesicle was found.
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'''Preparations''' <br>
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Three POPC/dodecane 10ml suspensions were prepared.
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==Cloning of Biobricks (for Maxiprep)==
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#Placed 100 ml of LB in a 500 ml conical flask
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#Innoculated 1 ml of culture into LB media in flask
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#Incubated overnight at 37 °C
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<font size=-2>
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*25. BBa_J37032 [pLux-GFP]
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*13. BBa_B0015 [ ]
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</font>
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Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Maxiprep | Maxiprep] in the general protocols page
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==Transformation of Biobricks==
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#1 biobrick was transformed into XL1-Blue
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#Cells were grown for half an hour and plated
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#Plates were incubated at 37&deg;C overnight
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*Transformed in XL1-Blue<font size=-2>
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**pSB1A3 [pSB1A3] (re-clone)</font>
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Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electropotion | Electroporation] in the general protocols page
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Latest revision as of 15:27, 26 October 2007

10 September 2007

Vesicles

Results

Sample 8, produced on 04.09.2007 was looked at again under the fluorescence microscope, but this time only a single 2μm vesicle was found.

Preparations

Three POPC/dodecane 10ml suspensions were prepared.

Cloning of Biobricks (for Maxiprep)

  1. Placed 100 ml of LB in a 500 ml conical flask
  2. Innoculated 1 ml of culture into LB media in flask
  3. Incubated overnight at 37 °C

  • 25. BBa_J37032 [pLux-GFP]
  • 13. BBa_B0015 [ ]

Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Maxiprep | Maxiprep] in the general protocols page

Transformation of Biobricks

  1. 1 biobrick was transformed into XL1-Blue
  2. Cells were grown for half an hour and plated
  3. Plates were incubated at 37°C overnight
  • Transformed in XL1-Blue
    • pSB1A3 [pSB1A3] (re-clone)

Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electropotion | Electroporation] in the general protocols page

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