Week 17

From 2007.igem.org

(Difference between revisions)
 
(10 intermediate revisions not shown)
Line 1: Line 1:
::'''10/22/07'''
::'''10/22/07'''
 +
*Ligation:
 +
::-[http://partsregistry.org/Part:BBa_I763032 I763032] (on [http://partsregistry.org/Part:pSB4A3 pSB4A3]);
 +
 +
*Transformation:
 +
::-[http://partsregistry.org/Part:BBa_I763032 I763032] (on [http://partsregistry.org/Part:pSB4A3 pSB4A3]);
 +
 +
*We inoculate:
 +
::-[http://partsregistry.org/Part:BBa_I763034 I763034] (on high copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763031 I763031] (on low copy plasmid), 3 different inocules using 3 different colonies;
 +
::-[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (high copy plasmid).
 +
::-[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (low copy plasmid).
Line 5: Line 16:
::'''10/23/07'''
::'''10/23/07'''
 +
*Miniprep:
 +
::-[http://partsregistry.org/Part:BBa_I763034 I763034] (on high copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763029 I763029] (high copy plasmid).
 +
::-[http://partsregistry.org/Part:BBa_I763029 I763029] (low copy plasmid).
 +
*Control Digestion for results impagination:
 +
::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763036 I763036];
 +
::-[http://partsregistry.org/Part:BBa_I763019 I763019];
 +
::-[http://partsregistry.org/Part:BBa_I763032 I763032];
 +
::-[http://partsregistry.org/Part:BBa_I763025 I763025] (low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763027 I763027];
 +
::-[http://partsregistry.org/Part:BBa_I763029 I763029] (low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763029 I763029] (high copy plasmid).
 +
*Inoculate [http://partsregistry.org/Part:BBa_I763032 I763032] (on [http://partsregistry.org/Part:pSB4A3 pSB4A3]).
-
::'''10/24/07'''
 
 +
::'''10/24/07'''
 +
 +
Data elaboration and wiki updating
::'''10/25/07'''
::'''10/25/07'''
-
 
+
Data elaboration and wiki updating
      
      
::'''10/26/07'''
::'''10/26/07'''
 +
Data elaboration and wiki updating
-
 
+
[[Bologna#Diary | Back]]
-
[[Bologna | Back]]
+

Latest revision as of 15:50, 26 October 2007

10/22/07
  • Ligation:
-[http://partsregistry.org/Part:BBa_I763032 I763032] (on [http://partsregistry.org/Part:pSB4A3 pSB4A3]);
  • Transformation:
-[http://partsregistry.org/Part:BBa_I763032 I763032] (on [http://partsregistry.org/Part:pSB4A3 pSB4A3]);
  • We inoculate:
-[http://partsregistry.org/Part:BBa_I763034 I763034] (on high copy plasmid);
-[http://partsregistry.org/Part:BBa_I763031 I763031] (on low copy plasmid), 3 different inocules using 3 different colonies;
-[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (high copy plasmid).
-[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (low copy plasmid).



10/23/07
  • Miniprep:
-[http://partsregistry.org/Part:BBa_I763034 I763034] (on high copy plasmid);
-[http://partsregistry.org/Part:BBa_I763029 I763029] (high copy plasmid).
-[http://partsregistry.org/Part:BBa_I763029 I763029] (low copy plasmid).
  • Control Digestion for results impagination:
-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763036 I763036];
-[http://partsregistry.org/Part:BBa_I763019 I763019];
-[http://partsregistry.org/Part:BBa_I763032 I763032];
-[http://partsregistry.org/Part:BBa_I763025 I763025] (low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763027 I763027];
-[http://partsregistry.org/Part:BBa_I763029 I763029] (low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763029 I763029] (high copy plasmid).
  • Inoculate [http://partsregistry.org/Part:BBa_I763032 I763032] (on [http://partsregistry.org/Part:pSB4A3 pSB4A3]).



10/24/07

Data elaboration and wiki updating


10/25/07

Data elaboration and wiki updating


10/26/07

Data elaboration and wiki updating


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