Week 15

From 2007.igem.org

(Difference between revisions)

Latest revision as of 15:51, 26 October 2007

10/08/07

We inoculate:

-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);

-E.Coli;

-[http://partsregistry.org/Part:BBa_I763032 I763032] (high copy plasmid).

Digestion of:

-[http://partsregistry.org/Part:BBa_I763035 I763035] with Eco/Pst1;

-[http://partsregistry.org/Part:BBa_I763007 I763007] with Eco/spe;

-[http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Xba;

-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Spe/Xba.

Ligation for:

-[http://partsregistry.org/Part:BBa_I763035 I763035]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];

-[http://partsregistry.org/Part:BBa_I763007 I763007]+[http://partsregistry.org/Part:BBa_I763026 I763026];

-[http://partsregistry.org/Part:BBa_I763007 I763007]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];

-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3].

Run on electrophoresis gel.
[http://partsregistry.org/Part:BBa_I763007 I763007] and [http://partsregistry.org/Part:BBa_I763026 I763026] band extraction.
O/N ligation for:

-[http://partsregistry.org/Part:BBa_I763026 I763026]+[http://partsregistry.org/Part:BBa_I763007 I763007];

-[http://partsregistry.org/Part:BBa_I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];

-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];

-[http://partsregistry.org/Part:BBa_I763007 I763007]+[http://partsregistry.org/Part:pSB4A3 pSB4A3].

We inoculate:

-[http://partsregistry.org/Part:BBa_I763031 I763031](low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763016 I763016](low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763032 I763032] (high copy plasmid).

10/09/07

Transformation of:

-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007];

-[http://partsregistry.org/Part:BBa_I763024 I763024] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763032 I763032] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid).

We inoculate in 6ml:

-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid).

Ligation for:

-[http://partsregistry.org/Part:BBa_I763016 I763016] (Eco/Spe)+GFP.(Eco/Xba).

10/10/07

Miniprep of:

-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid).

Digestion for :

-[http://partsregistry.org/Part:BBa_I763031 I763031] with Xba/Pst1;

-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid) with Spe/Pst1;

Run on electrophoresis gel and [http://partsregistry.org/Part:I763016 I763016] band extraction.
Ligation for :

-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid)+GFP;

-[http://partsregistry.org/Part:BBa_I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];

-[http://partsregistry.org/Part:BBa_I763024 I763024]+[http://partsregistry.org/Part:BBa_C0012 C0012] (high copy plasmid).

We inoculate in 6ml:

-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007](high copy plasmid);

-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763024 I763024] (low copy plasmid).

10/11/07

Stock preparation (500ul) of:

-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007](high copy plasmid);

-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763024 I763024] (low copy plasmid).

Miniprep of:

-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763007 I763007](high copy plasmid);

-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763024 I763024] (low copy plasmid).

Fluorescence test of:

-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007] (high copy plasmid);

-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid).

Control digestion of:

-[http://partsregistry.org/Part:BBa_I763031 I763031] with Xba/Pst1;

-[http://partsregistry.org/Part:BBa_I763016 I763016] with Xba/Pst1 and Spe/Xba;

-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007] with Xba/Pst1 and Spe/Xba;

-[http://partsregistry.org/Part:BBa_I763024 I763024] with Xba/Pst1;

-[http://partsregistry.org/Part:BBa_I763007 I763007] with Xba/Pst1.

Run on electrophoresis gel and band extraction.
Transformation of:

-[http://partsregistry.org/Part:BBa_I763019 I763019];

-[http://partsregistry.org/Part:BBa_I763024 I763024]+[http://partsregistry.org/Part:BBa_C0012 C0012];

-[http://partsregistry.org/Part:BBa_I763032 I763032].

We inoculate

-[http://partsregistry.org/Part:BBa_I763019 I763019].

10/12/07

Miniprep of:

-[http://partsregistry.org/Part:BBa_I763019 I763019].

Digestion of:

-[http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Pst1;

-[http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Xba.

Run on electrophoresis gel.
[http://partsregistry.org/Part:BBa_I763019 I763019] (Xba/Pst1) and [http://partsregistry.org/Part:BBa_I763019 I763019] (Eco/Xba) band extraction.

Back

@@ Line 1: / Line 1: @@
-::'''10/01/07'''
+::'''10/08/07'''
+*We inoculate:
+::-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);
+::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
+::-E.Coli;
+::-[http://partsregistry.org/Part:BBa_I763032 I763032] (high copy plasmid).
+*Digestion of:
+::-[http://partsregistry.org/Part:BBa_I763035 I763035] with Eco/Pst1;
+::-[http://partsregistry.org/Part:BBa_I763007 I763007] with Eco/spe;
+::-[http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Xba;
+::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Spe/Xba.
+*Ligation for:
+::-[http://partsregistry.org/Part:BBa_I763035 I763035]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
+::-[http://partsregistry.org/Part:BBa_I763007 I763007]+[http://partsregistry.org/Part:BBa_I763026 I763026];
+::-[http://partsregistry.org/Part:BBa_I763007 I763007]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
+::-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3].
+*Run on electrophoresis gel.
+*[http://partsregistry.org/Part:BBa_I763007 I763007] and [http://partsregistry.org/Part:BBa_I763026 I763026] band extraction.
+*O/N ligation for:
+::-[http://partsregistry.org/Part:BBa_I763026 I763026]+[http://partsregistry.org/Part:BBa_I763007 I763007];
+::-[http://partsregistry.org/Part:BBa_I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
+::-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
+::-[http://partsregistry.org/Part:BBa_I763007 I763007]+[http://partsregistry.org/Part:pSB4A3 pSB4A3].
+*We inoculate:
+::-[http://partsregistry.org/Part:BBa_I763031 I763031](low copy plasmid);
+::-[http://partsregistry.org/Part:BBa_I763016 I763016](low copy plasmid);
+::-[http://partsregistry.org/Part:BBa_I763032 I763032] (high copy plasmid).
+::'''10/09/07'''
+*Transformation of:
+::-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007];
+::-[http://partsregistry.org/Part:BBa_I763024 I763024] (low copy plasmid);
+::-[http://partsregistry.org/Part:BBa_I763032 I763032] (low copy plasmid);
+::-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid).
+*We inoculate in 6ml:
+::-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);
+::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid).
+*Ligation for:
+::-[http://partsregistry.org/Part:BBa_I763016 I763016] (Eco/Spe)+GFP.(Eco/Xba).
-::'''10/02/07'''
+::'''10/10/07'''
+*Miniprep of:
+::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
+::-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid).
+*Digestion for :
+::-[http://partsregistry.org/Part:BBa_I763031 I763031] with Xba/Pst1;
+::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid) with Spe/Pst1;
+*Run on electrophoresis gel and [http://partsregistry.org/Part:I763016 I763016] band extraction.
+*Ligation for :
+::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid)+GFP;
+::-[http://partsregistry.org/Part:BBa_I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
+::-[http://partsregistry.org/Part:BBa_I763024 I763024]+[http://partsregistry.org/Part:BBa_C0012 C0012] (high copy plasmid).
+*We inoculate in 6ml:
+::-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);
+::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
+::-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007](high copy plasmid);
+::-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid);
+::-[http://partsregistry.org/Part:BBa_I763024 I763024] (low copy plasmid).
+::'''10/11/07'''
+*Stock preparation (500ul) of:
+::-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);
+::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
+::-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007](high copy plasmid);
+::-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid);
+::-[http://partsregistry.org/Part:BBa_I763024 I763024] (low copy plasmid).
+*Miniprep of:
+::-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);
+::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
+::-[http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763007 I763007](high copy plasmid);
+::-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid);
+::-[http://partsregistry.org/Part:BBa_I763024 I763024] (low copy plasmid).
+*Fluorescence test of:
+::-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);
+::-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007] (high copy plasmid);
+::-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid).
+*Control digestion of:
+::-[http://partsregistry.org/Part:BBa_I763031 I763031] with Xba/Pst1;
+::-[http://partsregistry.org/Part:BBa_I763016 I763016] with Xba/Pst1 and Spe/Xba;
+::-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007] with Xba/Pst1 and Spe/Xba;
+::-[http://partsregistry.org/Part:BBa_I763024 I763024] with Xba/Pst1;
+::-[http://partsregistry.org/Part:BBa_I763007 I763007] with Xba/Pst1.
+*Run on electrophoresis gel and band extraction.
+*Transformation of:
+::-[http://partsregistry.org/Part:BBa_I763019 I763019];
+::-[http://partsregistry.org/Part:BBa_I763024 I763024]+[http://partsregistry.org/Part:BBa_C0012 C0012];
+::-[http://partsregistry.org/Part:BBa_I763032 I763032].
+*We inoculate
+::-[http://partsregistry.org/Part:BBa_I763019 I763019].
+::'''10/12/07'''
+*Miniprep of:
+::-[http://partsregistry.org/Part:BBa_I763019 I763019].
+*Digestion of:
+::-[http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Pst1;
+::-[http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Xba.
+*Run on electrophoresis gel.
+*[http://partsregistry.org/Part:BBa_I763019 I763019] (Xba/Pst1) and [http://partsregistry.org/Part:BBa_I763019 I763019] (Eco/Xba) band extraction.
-::'''10/03/07'''
+[[Bologna#Diary | Back]]
-::'''10/04/07'''
-::'''10/05/07'''
-[[Bologna | Back]]

Views

Personal tools

Week 15

From 2007.igem.org

Latest revision as of 15:51, 26 October 2007