Week 14
From 2007.igem.org
(Difference between revisions)
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*Digestion of [http://partsregistry.org/Part:pSB4A3 pSB4A3]; | *Digestion of [http://partsregistry.org/Part:pSB4A3 pSB4A3]; | ||
*Run on electrophoresis gel. | *Run on electrophoresis gel. | ||
| - | *[http://partsregistry.org/Part: | + | *[http://partsregistry.org/Part:pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763033 I763033] band extraction. |
*Ligations for: | *Ligations for: | ||
::-[http://partsregistry.org/Part:BBa_I763004 I763004]+[http://partsregistry.org/Part:BBa_I763033 I763033]; | ::-[http://partsregistry.org/Part:BBa_I763004 I763004]+[http://partsregistry.org/Part:BBa_I763033 I763033]; | ||
| Line 20: | Line 20: | ||
::-[http://partsregistry.org/Part:BBa_I763031 I763031] with Eco/Pst1; | ::-[http://partsregistry.org/Part:BBa_I763031 I763031] with Eco/Pst1; | ||
::-[http://partsregistry.org/Part:BBa_I763036 I763036] with Eco/Pst1; | ::-[http://partsregistry.org/Part:BBa_I763036 I763036] with Eco/Pst1; | ||
| - | ::-[http://partsregistry.org/Part: | + | ::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Eco/Pst1. |
*Run on electrophoresis gel. | *Run on electrophoresis gel. | ||
| - | *[http://partsregistry.org/Part:BBa_I763031 I763031], [http://partsregistry.org/Part:BBa_I763036 I763036] and [http://partsregistry.org/Part: | + | *[http://partsregistry.org/Part:BBa_I763031 I763031], [http://partsregistry.org/Part:BBa_I763036 I763036] and [http://partsregistry.org/Part:pSB4A3 pSB4A3] band extraction. |
*Transformations of: | *Transformations of: | ||
::-[http://partsregistry.org/Part:BBa_I763034 I763034]; | ::-[http://partsregistry.org/Part:BBa_I763034 I763034]; | ||
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::-[http://partsregistry.org/Part:BBa_I763032 I763032]. | ::-[http://partsregistry.org/Part:BBa_I763032 I763032]. | ||
*Ligations for: | *Ligations for: | ||
| - | ::-[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part: | + | ::-[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3]; |
| - | ::-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part: | + | ::-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3]; |
'''Experimental Setup''' | '''Experimental Setup''' | ||
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::'''10/04/07''' | ::'''10/04/07''' | ||
*Trasformation of: | *Trasformation of: | ||
| - | ::-[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part: | + | ::-[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3]; |
| - | ::-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part: | + | ::-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3]; |
| - | *We inoculate [http://partsregistry.org/Part:BBa_I763034 I763034], [http://partsregistry.org/Part:BBa_I763031 I763031] + [http://partsregistry.org/Part: | + | *We inoculate [http://partsregistry.org/Part:BBa_I763034 I763034], [http://partsregistry.org/Part:BBa_I763031 I763031] + [http://partsregistry.org/Part:pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3] (in 6 ml). |
| Line 61: | Line 61: | ||
| - | [[Bologna | Back]] | + | [[Bologna#Diary | Back]] |
Latest revision as of 15:51, 26 October 2007
- 10/01/07
- Meeting: Ethical issue.
- Work on image segmentation algorithm.
- 10/02/07
- Digestion of [http://partsregistry.org/Part:pSB4A3 pSB4A3];
- Run on electrophoresis gel.
- [http://partsregistry.org/Part:pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763033 I763033] band extraction.
- Ligations for:
- -[http://partsregistry.org/Part:BBa_I763004 I763004]+[http://partsregistry.org/Part:BBa_I763033 I763033];
- -[http://partsregistry.org/Part:BBa_I763012 I763012]+[http://partsregistry.org/Part:BBa_I763020 I763020][http://partsregistry.org/Part:BBa_I763032 (I763032)].
- 10/03/07
- Digestions of:
- -[http://partsregistry.org/Part:BBa_I763032 I763032] with Eco/Pst1;
- -[http://partsregistry.org/Part:BBa_I763031 I763031] with Eco/Pst1;
- -[http://partsregistry.org/Part:BBa_I763036 I763036] with Eco/Pst1;
- -[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Eco/Pst1.
- Run on electrophoresis gel.
- [http://partsregistry.org/Part:BBa_I763031 I763031], [http://partsregistry.org/Part:BBa_I763036 I763036] and [http://partsregistry.org/Part:pSB4A3 pSB4A3] band extraction.
- Transformations of:
- -[http://partsregistry.org/Part:BBa_I763034 I763034];
- -[http://partsregistry.org/Part:BBa_I763031 I763031];
- -[http://partsregistry.org/Part:BBa_I763032 I763032].
- Ligations for:
- -[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
- -[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
Experimental Setup
- To have an higher precision for our measurements, we have decided to get, for the same field of view on the same sample of bacteria solution, either a short film segment (from which we can extract frames) and a Microsoft Excel file, which contains a ten seconds interval of values from the photomultiplier output, taken at a 100Hz frequency.
- In order to have the same focus for the microscope's oculars, the photocamera and the photomultiplier we have to calibrate our system by changing the position of both camera and photomultiplier.
- Finally, we check that the photomultiplier field of view is the same size as the microscope's one: by moving the appropiate diaphragms, we adjust the previously done settings.
- 10/04/07
- Trasformation of:
- -[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
- -[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
- We inoculate [http://partsregistry.org/Part:BBa_I763034 I763034], [http://partsregistry.org/Part:BBa_I763031 I763031] + [http://partsregistry.org/Part:pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3] (in 6 ml).
- 10/05/07
- Stock preparation (500 ul) of [http://partsregistry.org/Part:I763034 I763034],[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3].
- Miniprep of [http://partsregistry.org/Part:BBa_I763034 I763034], [http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3].
- Control digestion of:
- -[http://partsregistry.org/Part:BBa_I763034 I763034] with Xba/Pst1;
- -[http://partsregistry.org/Part:BBa_I763031 I763031] with Eco/Xba and Xba/Pst1;
- -[http://partsregistry.org/Part:BBa_I763032 I763032] with Eco/Xba and Xba/Pst1.
- Run on electrophoresis gel and [http://partsregistry.org/Part:BBa_I763032 I763032] (Eco/Xba and Xba/Pst1) band extraction.
