Week 10

From 2007.igem.org

(Difference between revisions)

Latest revision as of 15:53, 26 October 2007

09/03/07

Miniprep for:

-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S0100 S0100];

-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S03520 S03520];

-[http://partsregistry.org/Part:BBa_I763005 I763005]+[http://partsregistry.org/Part:BBa_I763015 I763015];

-[http://partsregistry.org/Part:BBa_J52034 J52034]+[http://partsregistry.org/Part:BBa_E0022 E0022];

Digestion for:

-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S0100 S0100] with Eco/Spe;

-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S03520 S03520] with Eco/Spe;

-[http://partsregistry.org/Part:BBa_I763005 I763005]+[http://partsregistry.org/Part:BBa_I763015 I763015] with Spe/Pst1;

-[http://partsregistry.org/Part:BBa_J52034 J52034]+[http://partsregistry.org/Part:BBa_E0022 E0022] with Spe/Pst1;

-[http://partsregistry.org/Part:BBa_B0015 B0015] with Eco/Xba;

Band extraction from gel for all digestion;
Ligation for:

-[http://partsregistry.org/Part:BBa_I763025 I763025]+[http://partsregistry.org/Part:BBa_B0015 B0015]([http://partsregistry.org/Part:BBa_I763026 I763026]);

-[http://partsregistry.org/Part:BBa_I763024 I763024] +[http://partsregistry.org/Part:BBa_B0015 B0015]([http://partsregistry.org/Part:BBa_I763030 I763030]);

-[http://partsregistry.org/Part:BBa_I763016 I763016]+[http://partsregistry.org/Part:BBa_I763020 I763020]([http://partsregistry.org/Part:BBa_I763019 I763019]);

-[http://partsregistry.org/Part:BBa_I763009 I763009]+[http://partsregistry.org/Part:BBa_P0412 P0412]([http://partsregistry.org/Part:BBa_I763021 I763021]);

-[http://partsregistry.org/Part:BBa_I763009 I763009]+[http://partsregistry.org/Part:BBa_S0100 S0100]([http://partsregistry.org/Part:BBa_I763023 I763023]);

-[http://partsregistry.org/Part:BBa_I763009 I763009]+[http://partsregistry.org/Part:BBa_S03520 S03520]([http://partsregistry.org/Part:BBa_I763022 I763022]);

-[http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_I763020 I763020]([http://partsregistry.org/Part:BBa_I763011 I763011]);

09/04/07

There are colonies for every ligations.
Meeting.

09/05/07

Miniprep for:

- [http://partsregistry.org/Part:BBa_I763016 I763016]+ [http://partsregistry.org/Part:BBa_I763020 I763020] ([http://partsregistry.org/Part:BBa_I763019 I763019]);

- [http://partsregistry.org/Part:BBa_I763025 I763025]+ [http://partsregistry.org/Part:BBa_B0015 B0015] ([http://partsregistry.org/Part:BBa_I763026 I763026]);

- [http://partsregistry.org/Part:BBa_I763024 I763024]+ [http://partsregistry.org/Part:BBa_B0015 B0015] ([http://partsregistry.org/Part:BBa_I763030 I763030]);

- [http://partsregistry.org/Part:BBa_R0051 R0051]+ [http://partsregistry.org/Part:BBa_I763020 I763020] ([http://partsregistry.org/Part:BBa_I763011 I763011]);

- [http://partsregistry.org/Part:BBa_I763009 I763009]+ [http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763021 I763021]);

- [http://partsregistry.org/Part:BBa_I763009 I763009]+ [http://partsregistry.org/Part:BBa_S03520 S03520]([http://partsregistry.org/Part:BBa_I763022 I763022]);

Digestion for:

- [http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Xba;

- [http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe;

- [http://partsregistry.org/Part:BBa_I763030 I763030] with Eco/Spe;

- [http://partsregistry.org/Part:BBa_I763011 I763011] with Xba/Pst1;

- [http://partsregistry.org/Part:BBa_R0051 R0051]+ [http://partsregistry.org/Part:BBa_I13507 I13507]([http://partsregistry.org/Part:BBa_I763007 I763007]) with Xba/Pst1;

- [http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe;

Control digestion for [http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Pst1;
Band extraction from gel for:

- [http://partsregistry.org/Part:BBa_I763019 I763019];

- [http://partsregistry.org/Part:BBa_I763026 I763026];

- [http://partsregistry.org/Part:BBa_I763011 I763011];

- [http://partsregistry.org/Part:BBa_R0051 R0051]+ [http://partsregistry.org/Part:BBa_I13507 I13507] ([http://partsregistry.org/Part:BBa_I763007 I763007]);

New control digestion for:

-[http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Spe;

-[http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Pst1;

-[http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe;

-[http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe;

-[http://partsregistry.org/Part:BBa_I763030 I763030] with Eco/Spe;

Band extraction for [http://partsregistry.org/Part:BBa_I763019 I763019] (digested with Xba/Spe and with Xba/Pst1), [http://partsregistry.org/Part:BBa_I763026 I763026];
Ligation O/N for:

-[http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763019 I763019]([http://partsregistry.org/Part:BBa_I763028 I763028]);

-[http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763004 I763004]([http://partsregistry.org/Part:BBa_I763027 I763027]);

09/06/07

Digestion for:

-[http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Xba;

-[http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe;

-[http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe.

Band extraction from electrophoresis gel for [http://partsregistry.org/Part:BBa_I763019 I763019] and [http://partsregistry.org/Part:BBa_I763026 I763026].
Ligation for [http://partsregistry.org/Part:BBa_I763027 I763027] with new extracts.
Transformations for the new ligations.

09/07/07

Glycerol stocks preparation;
We check without plasmid bacteria growth curve to individuate a model parameter.

Back

@@ Line 2: / Line 2: @@
 *Miniprep for:
--R0040+S0100;
+-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S0100 S0100];
--R0040+S03520;
+-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S03520 S03520];
--I763005+I763015;
+-[http://partsregistry.org/Part:BBa_I763005 I763005]+[http://partsregistry.org/Part:BBa_I763015 I763015];
--J52034+E0022;
+-[http://partsregistry.org/Part:BBa_J52034 J52034]+[http://partsregistry.org/Part:BBa_E0022 E0022];
 *Digestion for:
--R0040+S0100 with Eco/Spe;
+-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S0100 S0100] with Eco/Spe;
--R0040+S03520 with Eco/Spe;
+-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S03520 S03520] with Eco/Spe;
--I763005+I763015 with Spe/Pst1;
+-[http://partsregistry.org/Part:BBa_I763005 I763005]+[http://partsregistry.org/Part:BBa_I763015 I763015] with Spe/Pst1;
--J52034+E0022with Spe/Pst1;
+-[http://partsregistry.org/Part:BBa_J52034 J52034]+[http://partsregistry.org/Part:BBa_E0022 E0022] with Spe/Pst1;
--B0015 with Eco/Xba;
+-[http://partsregistry.org/Part:BBa_B0015 B0015] with Eco/Xba;
-*Band extraction;
+*Band extraction from gel for all digestion;
 *Ligation for:
--I763025+B0015(I763026);
+-[http://partsregistry.org/Part:BBa_I763025 I763025]+[http://partsregistry.org/Part:BBa_B0015 B0015]([http://partsregistry.org/Part:BBa_I763026 I763026]);
--I763024+B0015(I763030);
+-[http://partsregistry.org/Part:BBa_I763024 I763024] +[http://partsregistry.org/Part:BBa_B0015 B0015]([http://partsregistry.org/Part:BBa_I763030 I763030]);
--I763016+I763020(I763019);
+-[http://partsregistry.org/Part:BBa_I763016 I763016]+[http://partsregistry.org/Part:BBa_I763020 I763020]([http://partsregistry.org/Part:BBa_I763019 I763019]);
--I763009+P0412(I763021);
+-[http://partsregistry.org/Part:BBa_I763009 I763009]+[http://partsregistry.org/Part:BBa_P0412 P0412]([http://partsregistry.org/Part:BBa_I763021 I763021]);
--I763009+S0100(I763023);
+-[http://partsregistry.org/Part:BBa_I763009 I763009]+[http://partsregistry.org/Part:BBa_S0100 S0100]([http://partsregistry.org/Part:BBa_I763023 I763023]);
--I763009+S03520(I763022);
+-[http://partsregistry.org/Part:BBa_I763009 I763009]+[http://partsregistry.org/Part:BBa_S03520 S03520]([http://partsregistry.org/Part:BBa_I763022 I763022]);
--R0051+I763020(I763011);
+-[http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_I763020 I763020]([http://partsregistry.org/Part:BBa_I763011 I763011]);
 ::'''09/04/07'''
 *There are colonies for every ligations.
+*Meeting.
@@ Line 47: / Line 47: @@
 Miniprep for:
--I763016+I763020(I763019);
+- [http://partsregistry.org/Part:BBa_I763016 I763016]+ [http://partsregistry.org/Part:BBa_I763020 I763020] ([http://partsregistry.org/Part:BBa_I763019 I763019]);
--I763025+B0015(I763026);
+- [http://partsregistry.org/Part:BBa_I763025 I763025]+ [http://partsregistry.org/Part:BBa_B0015 B0015] ([http://partsregistry.org/Part:BBa_I763026 I763026]);
--I763024+B0015(I763030);
+- [http://partsregistry.org/Part:BBa_I763024 I763024]+ [http://partsregistry.org/Part:BBa_B0015 B0015] ([http://partsregistry.org/Part:BBa_I763030 I763030]);
--R0051+I763020(I763011);
+- [http://partsregistry.org/Part:BBa_R0051 R0051]+ [http://partsregistry.org/Part:BBa_I763020 I763020] ([http://partsregistry.org/Part:BBa_I763011 I763011]);
--I763009+P0412(I763021);
+- [http://partsregistry.org/Part:BBa_I763009 I763009]+ [http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763021 I763021]);
--I763009+S03520(I763022);
+- [http://partsregistry.org/Part:BBa_I763009 I763009]+ [http://partsregistry.org/Part:BBa_S03520 S03520]([http://partsregistry.org/Part:BBa_I763022 I763022]);
 *Digestion for:
--I763019 with Eco/Xba;
+- [http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Xba;
--I763026 with Eco/Spe;
+- [http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe;
--I763030 with Eco/Spe;
+- [http://partsregistry.org/Part:BBa_I763030 I763030] with Eco/Spe;
--I763011 with Xba/Pst1;
+- [http://partsregistry.org/Part:BBa_I763011 I763011] with Xba/Pst1;
--R0051+I13507(I763007) with Xba/Pst1;
+- [http://partsregistry.org/Part:BBa_R0051 R0051]+ [http://partsregistry.org/Part:BBa_I13507 I13507]([http://partsregistry.org/Part:BBa_I763007 I763007]) with Xba/Pst1;
--I763021 with Eco/Spe;
+- [http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe;
-*Control digestion for I763019 with Eco/Pst1;
+*Control digestion for [http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Pst1;
-*Band extraction for
+*Band extraction from gel for:
--I763019;
+- [http://partsregistry.org/Part:BBa_I763019 I763019];
--I763026;
+- [http://partsregistry.org/Part:BBa_I763026 I763026];
--I763011;
+- [http://partsregistry.org/Part:BBa_I763011 I763011];
--R0051+I13507(I763007);
+- [http://partsregistry.org/Part:BBa_R0051 R0051]+ [http://partsregistry.org/Part:BBa_I13507 I13507] ([http://partsregistry.org/Part:BBa_I763007 I763007]);
 *New control digestion for:
--I763019 with Xba/Spe;
+-[http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Spe;
--I763019 with Xba/Pst1;
+-[http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Pst1;
--I763021 with Eco/Spe;
+-[http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe;
--I763026 with Eco/Spe;
+-[http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe;
--I763030 with Eco/Spe;
+-[http://partsregistry.org/Part:BBa_I763030 I763030] with Eco/Spe;
-*Band extraction for I763019 (digested with Xba/Spe and with Xba/Pst1), I763026;
+*Band extraction for [http://partsregistry.org/Part:BBa_I763019 I763019] (digested with Xba/Spe and with Xba/Pst1), [http://partsregistry.org/Part:BBa_I763026 I763026];
 *Ligation O/N for:
--I763026+I763019(I763028);
+-[http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763019 I763019]([http://partsregistry.org/Part:BBa_I763028 I763028]);
--I763026+I763004(I763027);
+-[http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763004 I763004]([http://partsregistry.org/Part:BBa_I763027 I763027]);
 ::'''09/06/07'''
+Digestion for:
+-[http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Xba;
+-[http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe;
+-[http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe.
+*Band extraction from electrophoresis gel for [http://partsregistry.org/Part:BBa_I763019 I763019] and [http://partsregistry.org/Part:BBa_I763026 I763026].
+*Ligation for [http://partsregistry.org/Part:BBa_I763027 I763027] with new extracts.
+*Transformations for the new ligations.
 ::'''09/07/07'''
+*Glycerol stocks preparation;
+*We check without plasmid [[bacteria]] [[Growth Curve - without plasmid bacteria | growth curve]] to individuate a model parameter.
-[[Bologna|Back]]
+[[Bologna#Diary | Back]]

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Week 10

From 2007.igem.org

Latest revision as of 15:53, 26 October 2007