Melbourne
From 2007.igem.org
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<center>[[Image:Melbourne banner.png|800px]]</center> | <center>[[Image:Melbourne banner.png|800px]]</center> | ||
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'''The [[Melbourne/team|team]] welcomes you to the Melbourne University IGEM2007 Wiki!''' | '''The [[Melbourne/team|team]] welcomes you to the Melbourne University IGEM2007 Wiki!''' | ||
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+ | [[Melbourne/Melbourne overview|Project overview]] | ||
- | + | [[Melbourne/Background|Background & references]] | |
+ | [[Melbourne/Plan|Original Plan]] | ||
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+ | [[Image:Melbourne-team1.jpg|120px]] | ||
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+ | [[Image:Melbourne-team7.jpg|120px]] | ||
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+ | [[Image:Melbourne-team5.jpg|120px]] | ||
</td> | </td> | ||
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- | + | '''''Project''''' | |
+ | This project aims to use light to form a solid fluorescent mass of E. coli where two light beams intersect in a suspension of cells. We've called our building system "Coliforming". | ||
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+ | '''''Motivation''''' | ||
+ | Possible applications in building complex scaffolds in tissue engineering. | ||
+ | |||
+ | |||
+ | '''''Requirements''''' | ||
+ | The system requires six biological components. | ||
+ | # Red photosensor | ||
+ | # Blue photosensor | ||
+ | # AND gate | ||
+ | # GFP fluorescent reporter | ||
+ | # Promotable surface expression of cadherins | ||
+ | # Gas vesicle expression to produce neutrally bouyant bacteria | ||
+ | |||
+ | |||
+ | '''''High Level System Design''''' | ||
+ | * Induce or constitutively express gas vesicles for neutral bouyancy | ||
+ | * If Red & Blue light detected, then promote adhesion molecules. | ||
+ | |||
+ | |||
+ | '''''Achievements''''' | ||
+ | * Produced bouyant bacteria biobrick.[[Melbourne/Lab GV Notebook|(Have a look)]] | ||
+ | * Produced parts of other biological components required. | ||
</td> | </td> | ||
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</tr></table> | </tr></table> | ||
- | <center><h2><font face="broadway,verdana"> | + | <center><h2><font face="broadway,verdana">Procedures & Practical Matters</font></h2></center> |
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* [[Melbourne/Protocols for Standard Methods|Protocols for standard methods]] | * [[Melbourne/Protocols for Standard Methods|Protocols for standard methods]] | ||
* [[Melbourne/equipement|Fixed Equipement & tools]] | * [[Melbourne/equipement|Fixed Equipement & tools]] | ||
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* [[Melbourne/Software|Software links]] | * [[Melbourne/Software|Software links]] | ||
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<center><h2><font face="broadway,verdana">Sponsors</font></h2></center> | <center><h2><font face="broadway,verdana">Sponsors</font></h2></center> | ||
+ | <table cellpadding="5" border="0"><tr> | ||
+ | <td valign="top" width="60%"> | ||
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* University of Melbourne | * University of Melbourne | ||
* Faculty of Biomedical Engineering | * Faculty of Biomedical Engineering | ||
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+ | </td> | ||
+ | <td valign="top" width="40%"> | ||
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* Bio21 Institute | * Bio21 Institute | ||
- | * Vice Chancellor (Research) | + | * Vice Chancellor (Research) Office |
__NOTOC__ | __NOTOC__ |
Latest revision as of 16:29, 26 October 2007
The team welcomes you to the Melbourne University IGEM2007 Wiki!
"Coliforming"
Procedures & Practical Matters
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