Melbourne

From 2007.igem.org

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[[Image:Melbourne-team6.jpg|750px]]
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'''The [[Melbourne/team|team]] welcomes you to the Melbourne University IGEM2007 Wiki!'''
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! height="50pt" | <center>[[Image:Banner.png|800px]]</center>
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<center><font size="2"><font face = "arial">The newsletter of Imperial College London iGEM 2006</font></font></center>
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! [[IGEM:IMPERIAL/2006/Contributions|Our Contributions]] !! [[IGEM:IMPERIAL/2006/Before_the_Jamboree|To Do List]] !! [[IGEM:IMPERIAL/2006/project/Oscillator/project_browser|Biological Oscillator Parts]] !! [[IGEM:IMPERIAL/2006/project/Oscillator/Theoretical_Analyses/Results| Modelling]] !! [[IGEM:IMPERIAL/2006/Protocols|Protocols]] !! [[IGEM:IMPERIAL/2006/Resources|Resources]]
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<center><h2><font face="broadway,verdana">"Coliforming"</font></h2></center>
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==<font color=yellow><center> Breaking News </center></font>==
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*'''6 Nov 2006:''' <font color=yellow>Interested to join? more info </font> [[IGEM:IMPERIAL/2007| <font color=yellow><u>here</u>!</font>]]
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*'''5 Nov 2006:''' <font color=yellow>I.CoLi wins 1st Runner Up Prize at </font> [[IGEM:IMPERIAL/2006/Presentation| <font color=yellow><u>iGEM Jamboree</u>!</font>]]
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[[Melbourne/Melbourne overview|Project overview]]
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*'''30 Oct 2006:''' <font color=yellow>300+ pages documentation on OWW.</font> [https://2006.igem.org/wiki/index.php/Imperial_College_2006 <font color=yellow>New iGEM page</font>]
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[[Melbourne/Background|Background & references]]
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*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Breaking_News <font face="arial" font style="color: #FFFFFF" >News Archive</font>]
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[[Melbourne/Plan|Original Plan]]
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[[Image:Melbourne-team1.jpg|120px]]
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Location of our visitors
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<a href="http://clustrmaps.com/counter/maps.php?url=http://openwetware.org/wiki/IGEM:IMPERIAL/2006&type=small&clusters=no&map=world" id="clustrMapsLink"><img src="http://clustrmaps.com/counter/index2.php?url=http://openwetware.org/wiki/IGEM:IMPERIAL/2006" border=1 alt="Locations of visitors to this page"onError="this.onError=null; this.src='http://www.meetomatic.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://clustrmaps.com/'">
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[[Image:Melbourne-team7.jpg|120px]]
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==<font color=#2171B8>Imperial College London iGEM Project(s) 2006</font>==
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[[Image:Melbourne-team5.jpg|120px]]
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<big>[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser '''<font color=red>Engineering a Molecular Predation Oscillator.</font>''']
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{{Click || image=Oscillator_Icon.PNG | link=IGEM:IMPERIAL/2006/project/Oscillator/project_browser| width=300px | height=300px}}
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! <big>'''[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/popsblocker <font color=red>PoPs Blocker</font>] '''</big> !! <big>'''[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Bio_elec_interface <font color=red>Biological to Electrical Interface</font>]'''</big>
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</td>
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==<font color=yellow>Celebrities</font>==
 
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[[Image:IGEM GroupPhotos0015.JPG|200px|center]]
 
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Get to know the I.Coli team and their advisors:
 
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<showhide>
 
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'''<font face="arial" font style="color: yellow" >The Undergrads</font>'''
 
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__HIDER__
 
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<hide>
 
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*[[User:Cys|<font face="arial" font style="color: #FFFFFF" >Christin Sander</font>]]
 
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*[[User:da104|<font face="arial" font style="color: #FFFFFF" >Deepti Aswani</font>]]
 
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*[[User:Farah|<font face="arial" font style="color: #FFFFFF" >Farah Vohra</font>]]
 
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*[[User:Baijiongjun|<font face="arial" font style="color: #FFFFFF" >Jiongjun Bai</font>]]
 
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*[[User:Johnsy|<font face="arial" font style="color: #FFFFFF" >John Sy</font>]]
 
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*[[User:JohnChattaway|<font face="arial" font style="color: #FFFFFF" >John Chattaway</font>]]
 
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*[[User:Jonny|<font face="arial" font style="color: #FFFFFF" >Jonathan Wells</font>]]
 
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*[[User:Tom|<font face="arial" font style="color: #FFFFFF" >Tom Hinson</font>]]
 
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</hide></showhide>
 
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<showhide>
 
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'''<font face="arial" font style="color: yellow" >The Advisors</font>'''__HIDER__
 
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<hide>
 
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*[http://www3.imperial.ac.uk/people/r.kitney <font face="arial" font style="color: #FFFFFF" >Prof. Richard Kitney</font>]
 
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*[http://www3.imperial.ac.uk/people/p.freemont <font face="arial" font style="color: #FFFFFF" >Prof. Paul Freemont</font>]
 
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*[http://www3.imperial.ac.uk/people/d.mann <font face="arial" font style="color: #FFFFFF" >Dr. David Mann</font>]
 
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*<font face="arial" font style="color: #FFFFFF" >Kirsten Jensen</font>
 
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*[[User:Vincent|<font face="arial" font style="color: #FFFFFF" >Vincent Rouilly</font>]]
 
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*[[User:chuehloo|<font face="arial" font style="color: #FFFFFF" >Chueh-Loo Poh</font>]]
 
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*[[User:SxE00|<font face="arial" font style="color: #FFFFFF" >Matthieu Bultelle</font>]]
 
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</hide></showhide>
 
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[[IGEM:IMPERIAL/2006/Acknowledgments|<font face="arial" font style="color: #FFFFFF" >Acknowledgements</font>]]
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'''''Project'''''
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This project aims to use light to form a solid fluorescent mass of E. coli where two light beams intersect in a suspension of cells. We've called our building system "Coliforming".
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__NOTOC__
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==<font color=yellow>Activities</font>==
 
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</center>
 
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*[[IGEM:IMPERIAL/2006/Calendar|<font face="arial" font style="color: #FFFFFF" >Event Calendar</font>]]
 
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*[[IGEM:IMPERIAL/2006/Presentation|<font face="arial" font style="color: #FFFFFF" >MIT Jamboree</font>]]
 
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*[http://openwetware.org/wiki/BioSysBio:abstracts/2007/iGEM2006_Imperial_College <font face="arial" font style="color: #FFFFFF" >BioSysBio Conference, UK</font>]
 
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*[[IGEM:IMPERIAL/2006/Calendar/2006-7-26|<font face="arial" font style="color: #FFFFFF" >UK iGEM Teams Meeting in Cambridge</font>]]
 
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'''''Motivation'''''
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<center>
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Possible applications in building complex scaffolds in tissue engineering.
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==<font color=#2171B8>In the Wetlab</font>==
 
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</center>
 
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*[[IGEM:IMPERIAL/2006/LabCalendar| Lab Notebook]]
 
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*[[IGEM:IMPERIAL/2006/Protocols|Protocols]]
 
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*[[IGEM:IMPERIAL/2006/project/parts/lab_status| Lab Status]]
 
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*[[IGEM:IMPERIAL/2006/project/parts|Testing]]
 
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*[[IGEM:IMPERIAL/2006/project/sequencing|Sequencing of Parts]]
 
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*[[IGEM:IMPERIAL/2006/project/primers|Primers]]
 
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'''''Requirements'''''
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The system requires six biological components.
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# Red photosensor
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# Blue photosensor
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# AND gate
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# GFP fluorescent reporter
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# Promotable surface expression of cadherins
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# Gas vesicle expression to produce neutrally bouyant bacteria
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==<font color=#2171B8>Education</font>==
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'''''High Level System Design'''''
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</center>
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* Induce or constitutively express gas vesicles for neutral bouyancy
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*[[IGEM:IMPERIAL/2006/Brainstorming|Brainstorming]]
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* If Red & Blue light detected, then promote adhesion molecules.
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*[[IGEM:IMPERIAL/2006/Journal Club|Journal Club]]<br\>
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*[[IGEM:IMPERIAL/2006/Lecture Notes|Lecture Notes]]<br\>
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*[[IGEM:IMPERIAL/2006/Bibliography & Papers|Bibliography/Papers]]<br\>
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*[[IGEM:IMPERIAL/2006/Resources|Resources]]<br\>
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'''''Achievements'''''
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<center>
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* Produced bouyant bacteria biobrick.[[Melbourne/Lab GV Notebook|(Have a look)]]
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* Produced parts of other biological components required.
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==<font color=yellow>Entertainment</font>==
 
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</center>
 
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*[[IGEM:IMPERIAL/2006/Photos|<font face="arial" font style="color: #FFFFFF" >Photos</font>]]
 
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*[[IGEM:IMPERIAL/2006/Logo|<font face="arial" font style="color: #FFFFFF" >Logo & Team Shirts</font>]]
 
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*[[IGEM:IMPERIAL/2006/Quotes|<font face="arial" font style="color: #FFFFFF" >Inspirational Quotes</font>]]
 
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*[[IGEM:IMPERIAL/2006/The Talk Page|<font face="arial" font style="color: #FFFFFF" >Project Discussion</font>]]
 
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*[[IGEM:IMPERIAL/2006/Sandbox| <font face="arial" font style="color: #FFFFFF" >Sandbox</font>]]
 
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<center><h2><font face="broadway,verdana">Procedures & Practical Matters</font></h2></center>
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<td valign="top" width="50%">
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* [[Melbourne/Primary Reagents & disposables|Primary Reagents & disposables]]
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* [[Melbourne/Protocols for Secondary Reagents|Protocols for secondary Reagents and waste disposal]]
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* [[Melbourne/Protocols for Standard Methods|Protocols for standard methods]]
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* [[Melbourne/equipement|Fixed Equipement & tools]]
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* [[Melbourne/Software|Software links]]
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</td>
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<td valign="top" width="50%">
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* [[Melbourne/Lab Notebook|Lab notebook/diary]]
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* [[Melbourne/Parts used|Parts Used by Melbourne]]
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* [[Melbourne/Parts created|Parts Created by Melbourne]]
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* [[Melbourne/meeting minutes|Meeting Minutes]]
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<center><h2><font face="broadway,verdana">Sponsors</font></h2></center>
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* University of Melbourne
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* Faculty of Biomedical Engineering
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</td>
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* Bio21 Institute
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* Vice Chancellor (Research) Office
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__NOTOC__

Latest revision as of 16:29, 26 October 2007

Melbourne banner.png

Melbourne-team6.jpg The team welcomes you to the Melbourne University IGEM2007 Wiki!


"Coliforming"

Project overview

Background & references

Original Plan

Melbourne-team1.jpg

Melbourne-team7.jpg

Melbourne-team5.jpg


Project This project aims to use light to form a solid fluorescent mass of E. coli where two light beams intersect in a suspension of cells. We've called our building system "Coliforming".


Motivation Possible applications in building complex scaffolds in tissue engineering.


Requirements The system requires six biological components.

  1. Red photosensor
  2. Blue photosensor
  3. AND gate
  4. GFP fluorescent reporter
  5. Promotable surface expression of cadherins
  6. Gas vesicle expression to produce neutrally bouyant bacteria


High Level System Design

  • Induce or constitutively express gas vesicles for neutral bouyancy
  • If Red & Blue light detected, then promote adhesion molecules.


Achievements

  • Produced bouyant bacteria biobrick.(Have a look)
  • Produced parts of other biological components required.


Procedures & Practical Matters



Sponsors

  • University of Melbourne
  • Faculty of Biomedical Engineering
  • Bio21 Institute
  • Vice Chancellor (Research) Office


Retrieved from "http://2007.igem.org/wiki/index.php/Melbourne"