Melbourne

From 2007.igem.org

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'''The [[Melbourne/team|team]] welcomes you to the Melbourne University IGEM2007 Wiki!'''
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<center><font size="2"><font face = "arial">The University of Melbourne iGEM 2007</font></font></center>
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[[protocols]]
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<center><h2><font face="broadway,verdana">"Coliforming"</font></h2></center>
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[[Melbourne/Melbourne overview|Project overview]]
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[[Melbourne/Background|Background & references]]
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[[Melbourne/Plan|Original Plan]]
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[[Image:Melbourne-team1.jpg|120px]]
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[[Image:Melbourne-team7.jpg|120px]]
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[[Image:Melbourne-team5.jpg|120px]]
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'''''Project'''''
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This project aims to use light to form a solid fluorescent mass of E. coli where two light beams intersect in a suspension of cells. We've called our building system "Coliforming".
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'''''Motivation'''''
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Possible applications in building complex scaffolds in tissue engineering.
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'''''Requirements'''''
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The system requires six biological components.
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# Red photosensor
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# Blue photosensor
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# AND gate
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# GFP fluorescent reporter
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# Promotable surface expression of cadherins
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# Gas vesicle expression to produce neutrally bouyant bacteria
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'''''High Level System Design'''''
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* Induce or constitutively express gas vesicles for neutral bouyancy
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* If Red & Blue light detected, then promote adhesion molecules.
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'''''Achievements'''''
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* Produced bouyant bacteria biobrick.[[Melbourne/Lab GV Notebook|(Have a look)]]
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* Produced parts of other biological components required.
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<center><h2><font face="broadway,verdana">Procedures & Practical Matters</font></h2></center>
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* [[Melbourne/Primary Reagents & disposables|Primary Reagents & disposables]]
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* [[Melbourne/Protocols for Secondary Reagents|Protocols for secondary Reagents and waste disposal]]
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* [[Melbourne/Protocols for Standard Methods|Protocols for standard methods]]
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* [[Melbourne/equipement|Fixed Equipement & tools]]
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* [[Melbourne/Software|Software links]]
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* [[Melbourne/Lab Notebook|Lab notebook/diary]]
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* [[Melbourne/Parts used|Parts Used by Melbourne]]
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* [[Melbourne/Parts created|Parts Created by Melbourne]]
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* [[Melbourne/meeting minutes|Meeting Minutes]]
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<center><h2><font face="broadway,verdana">Sponsors</font></h2></center>
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* University of Melbourne
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* Faculty of Biomedical Engineering
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* Bio21 Institute
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* Vice Chancellor (Research) Office
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__NOTOC__

Latest revision as of 16:29, 26 October 2007

Melbourne banner.png

Melbourne-team6.jpg The team welcomes you to the Melbourne University IGEM2007 Wiki!


"Coliforming"

Project overview

Background & references

Original Plan

Melbourne-team1.jpg

Melbourne-team7.jpg

Melbourne-team5.jpg


Project This project aims to use light to form a solid fluorescent mass of E. coli where two light beams intersect in a suspension of cells. We've called our building system "Coliforming".


Motivation Possible applications in building complex scaffolds in tissue engineering.


Requirements The system requires six biological components.

  1. Red photosensor
  2. Blue photosensor
  3. AND gate
  4. GFP fluorescent reporter
  5. Promotable surface expression of cadherins
  6. Gas vesicle expression to produce neutrally bouyant bacteria


High Level System Design

  • Induce or constitutively express gas vesicles for neutral bouyancy
  • If Red & Blue light detected, then promote adhesion molecules.


Achievements

  • Produced bouyant bacteria biobrick.(Have a look)
  • Produced parts of other biological components required.


Procedures & Practical Matters



Sponsors

  • University of Melbourne
  • Faculty of Biomedical Engineering
  • Bio21 Institute
  • Vice Chancellor (Research) Office


Retrieved from "http://2007.igem.org/wiki/index.php/Melbourne"