Melbourne

From 2007.igem.org

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[[Image:Melbourne-team6.jpg|750px]]
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'''Welcome to the Melbourne University IGEM2007 Wiki!'''  
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'''The [[Melbourne/team|team]] welcomes you to the Melbourne University IGEM2007 Wiki!'''
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<center><h2><font face="broadway,verdana">Coliforming</font></h2></center>
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<center><h2><font face="broadway,verdana">"Coliforming"</font></h2></center>
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[[Melbourne/Melbourne overview|Project overview]]
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This project aims to use light to produce a solid fluorescent mass of ecoli where two light beams intersect in a suspension of cells.
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[[Melbourne/Background|Background & references]]
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[[Melbourne/Plan|Original Plan]]
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[[Image:Melbourne-team1.jpg|120px]]
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[[Image:Melbourne-team7.jpg|120px]]
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[[Image:Melbourne-team5.jpg|120px]]
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[[melb Background|Background]]
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[[melb Plan|Plan]]
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'''''Project'''''
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This project aims to use light to form a solid fluorescent mass of E. coli where two light beams intersect in a suspension of cells. We've called our building system "Coliforming".
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[[melb Achievements|Achievements]]
 
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[[melb Applications|Applications]]
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'''''Motivation'''''
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Possible applications in building complex scaffolds in tissue engineering.
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'''''Requirements'''''
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The system requires six biological components.
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# Red photosensor
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# Blue photosensor
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# AND gate
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# GFP fluorescent reporter
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# Promotable surface expression of cadherins
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# Gas vesicle expression to produce neutrally bouyant bacteria
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'''''High Level System Design'''''
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* Induce or constitutively express gas vesicles for neutral bouyancy
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* If Red & Blue light detected, then promote adhesion molecules.
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'''''Achievements'''''
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* Produced bouyant bacteria biobrick.[[Melbourne/Lab GV Notebook|(Have a look)]]
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* Produced parts of other biological components required.
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[[melb Future Prospects|Conclusions and Future Work]]
 
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<center><h2><font face="broadway,verdana">Lab Procedures</font></h2></center>
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<center><h2><font face="broadway,verdana">Procedures & Practical Matters</font></h2></center>
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* [[Melbourne/Primary Reagents & disposables|Primary Reagents & disposables]]
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* [[Melbourne/Protocols for Secondary Reagents|Protocols for secondary Reagents and waste disposal]]
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* [[Melbourne/Protocols for Standard Methods|Protocols for standard methods]]
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* [[Melbourne/equipement|Fixed Equipement & tools]]
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* [[Melbourne/Software|Software links]]
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* [[melb Primary|Primary Reagents]]
 
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* [[melb secondary|Protocols for secondary Reagents]]
 
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* [[melb tertiary|Protocols for standard methods]]
 
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* [[melb Experiment|Experiment report]]
 
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* [[melb Lab Notebook|Lab notebook/diary]]
 
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<h3><ul><li>'''Templates:'''</ul></h3>
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* [[melb Primary T|Primary Reagents]]
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* [[Melbourne/Lab Notebook|Lab notebook/diary]]
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* [[melb secondary T|Protocols for secondary Reagents]]
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* [[Melbourne/Parts used|Parts Used by Melbourne]]
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* [[melb tertiary T|Protocols for standard methods]]
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* [[Melbourne/Parts created|Parts Created by Melbourne]]
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* [[melb Experiment T|Experiment report]]
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* [[Melbourne/meeting minutes|Meeting Minutes]]
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<center><h2><font face="broadway,verdana">Other</font></h2></center>
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<center><h2><font face="broadway,verdana">Sponsors</font></h2></center>
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* [[melb News]]
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* [[melb Team Members]]
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* [[melb Relevant Papers]]
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* University of Melbourne
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* Faculty of Biomedical Engineering
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* Bio21 Institute
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* Vice Chancellor (Research) Office
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The team consists of [[melb undergrad|six undergraduate]] students, [[melb postgrad|two postgraduate]] students, and [[melb profs|two professors]].
 
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Latest revision as of 16:29, 26 October 2007

Melbourne banner.png

Melbourne-team6.jpg The team welcomes you to the Melbourne University IGEM2007 Wiki!


"Coliforming"

Project overview

Background & references

Original Plan

Melbourne-team1.jpg

Melbourne-team7.jpg

Melbourne-team5.jpg


Project This project aims to use light to form a solid fluorescent mass of E. coli where two light beams intersect in a suspension of cells. We've called our building system "Coliforming".


Motivation Possible applications in building complex scaffolds in tissue engineering.


Requirements The system requires six biological components.

  1. Red photosensor
  2. Blue photosensor
  3. AND gate
  4. GFP fluorescent reporter
  5. Promotable surface expression of cadherins
  6. Gas vesicle expression to produce neutrally bouyant bacteria


High Level System Design

  • Induce or constitutively express gas vesicles for neutral bouyancy
  • If Red & Blue light detected, then promote adhesion molecules.


Achievements

  • Produced bouyant bacteria biobrick.(Have a look)
  • Produced parts of other biological components required.


Procedures & Practical Matters



Sponsors

  • University of Melbourne
  • Faculty of Biomedical Engineering
  • Bio21 Institute
  • Vice Chancellor (Research) Office


Retrieved from "http://2007.igem.org/wiki/index.php/Melbourne"