Melbourne

From 2007.igem.org

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[[Image:Melbourne-team6.jpg|750px]]
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'''The [[melb team|team]] welcomes you to the Melbourne University IGEM2007 Wiki!'''
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'''The [[Melbourne/team|team]] welcomes you to the Melbourne University IGEM2007 Wiki!'''
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<center><h2><font face="broadway,verdana">Coliforming</font></h2></center>
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<center><h2><font face="broadway,verdana">"Coliforming"</font></h2></center>
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[[Melbourne/Melbourne overview|Project overview]]
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This project aims to use light to produce a solid fluorescent mass of ecoli where two light beams intersect in a suspension of cells.
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[[Melbourne/Background|Background & references]]
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[[Melbourne/Plan|Original Plan]]
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[[Image:Melbourne-team1.jpg|120px]]
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[[Image:Melbourne-team7.jpg|120px]]
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[[Image:Melbourne-team5.jpg|120px]]
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[[melb overview|Project overview (China workshop presentation)]]
 
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[[melb Background|Background & Pappers referenced]]
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[[melb Plan|Plan]]
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'''''Project'''''
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This project aims to use light to form a solid fluorescent mass of E. coli where two light beams intersect in a suspension of cells. We've called our building system "Coliforming".
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[[melb Achievements|Achievements]]
 
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[[melb Applications|Applications]]
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'''''Motivation'''''
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Possible applications in building complex scaffolds in tissue engineering.
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'''''Requirements'''''
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The system requires six biological components.
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# Red photosensor
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# Blue photosensor
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# AND gate
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# GFP fluorescent reporter
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# Promotable surface expression of cadherins
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# Gas vesicle expression to produce neutrally bouyant bacteria
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'''''High Level System Design'''''
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* Induce or constitutively express gas vesicles for neutral bouyancy
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* If Red & Blue light detected, then promote adhesion molecules.
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'''''Achievements'''''
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* Produced bouyant bacteria biobrick.[[Melbourne/Lab GV Notebook|(Have a look)]]
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* Produced parts of other biological components required.
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[[melb Future Prospects|Conclusions and Future Work]]
 
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<center><h2><font face="broadway,verdana">Lab Procedures, Equipement ,Safety, Location</font></h2></center>
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<center><h2><font face="broadway,verdana">Procedures & Practical Matters</font></h2></center>
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* [[melb Primary|Primary Reagents & disposables]]
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* [[Melbourne/Primary Reagents & disposables|Primary Reagents & disposables]]
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* [[melb secondary|Protocols for secondary Reagents]]
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* [[Melbourne/Protocols for Secondary Reagents|Protocols for secondary Reagents and waste disposal]]
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* [[melb tertiary|Protocols for standard methods]]
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* [[Melbourne/Protocols for Standard Methods|Protocols for standard methods]]
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* [[melb equipement|Fixed Equipement & tools]]
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* [[Melbourne/equipement|Fixed Equipement & tools]]
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* [[melb Experiment|Experiment report]]
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* [[Melbourne/Software|Software links]]
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* [[melb Lab Notebook|Lab notebook/diary]]
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* [[melb meeting minutes]]
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* [[Melbourne/Lab Notebook|Lab notebook/diary]]
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* [[melb promotional material]]
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* [[Melbourne/Parts used|Parts Used by Melbourne]]
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<h3><ul><li>'''Templates:'''</ul></h3>
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* [[Melbourne/Parts created|Parts Created by Melbourne]]
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* [[melb Primary T|Primary Reagents]]
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* [[Melbourne/meeting minutes|Meeting Minutes]]
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* [[melb secondary T|Protocols for secondary Reagents]]
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* [[melb tertiary T|Protocols for standard methods]]
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* [[melb Experiment T|Experiment report]]
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<center><h2><font face="broadway,verdana">Sponsors</font></h2></center>
<center><h2><font face="broadway,verdana">Sponsors</font></h2></center>
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* University of Melbourne
* University of Melbourne
* Faculty of Biomedical Engineering
* Faculty of Biomedical Engineering
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* Bio21 Institute
* Bio21 Institute
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* Vice Chancellor (Research) ’s Office  
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* Vice Chancellor (Research) Office  
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The team consists of [[melb undergrad|six undergraduate]] students, [[melb postgrad|two postgraduate]] students, and [[melb profs|two professors]].
 
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Latest revision as of 16:29, 26 October 2007

Melbourne banner.png

Melbourne-team6.jpg The team welcomes you to the Melbourne University IGEM2007 Wiki!


"Coliforming"

Project overview

Background & references

Original Plan

Melbourne-team1.jpg

Melbourne-team7.jpg

Melbourne-team5.jpg


Project This project aims to use light to form a solid fluorescent mass of E. coli where two light beams intersect in a suspension of cells. We've called our building system "Coliforming".


Motivation Possible applications in building complex scaffolds in tissue engineering.


Requirements The system requires six biological components.

  1. Red photosensor
  2. Blue photosensor
  3. AND gate
  4. GFP fluorescent reporter
  5. Promotable surface expression of cadherins
  6. Gas vesicle expression to produce neutrally bouyant bacteria


High Level System Design

  • Induce or constitutively express gas vesicles for neutral bouyancy
  • If Red & Blue light detected, then promote adhesion molecules.


Achievements

  • Produced bouyant bacteria biobrick.(Have a look)
  • Produced parts of other biological components required.


Procedures & Practical Matters



Sponsors

  • University of Melbourne
  • Faculty of Biomedical Engineering
  • Bio21 Institute
  • Vice Chancellor (Research) Office


Retrieved from "http://2007.igem.org/wiki/index.php/Melbourne"