Melbourne

From 2007.igem.org

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<center>[[Image:Melbourne banner.png|800px]]</center>
<center>[[Image:Melbourne banner.png|800px]]</center>
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[[Image:Melbourne-team6.jpg|750px]]
'''The [[Melbourne/team|team]] welcomes you to the Melbourne University IGEM2007 Wiki!'''
'''The [[Melbourne/team|team]] welcomes you to the Melbourne University IGEM2007 Wiki!'''
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<td valign="top" width="20%">
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[[Melbourne/Melbourne overview|Project overview]]
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This project aims to use light to produce a solid fluorescent mass of E. coli where two light beams intersect in a suspension of cells.
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[[Melbourne/Background|Background & references]]
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[[Melbourne/Plan|Original Plan]]
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[[Image:Melbourne-team1.jpg|120px]]
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[[Image:Melbourne-team7.jpg|120px]]
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[[Image:Melbourne-team5.jpg|120px]]
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[[melbourne overview|Project overview (China workshop presentation)]]
 
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[[melb:Background|Background & Papers referenced]]
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[[melb:Plan|Plan]]
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'''''Project'''''
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This project aims to use light to form a solid fluorescent mass of E. coli where two light beams intersect in a suspension of cells. We've called our building system "Coliforming".
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[[melb:Achievements|Achievements]]
 
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[[melb:Applications|Applications]]
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'''''Motivation'''''
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Possible applications in building complex scaffolds in tissue engineering.
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'''''Requirements'''''
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The system requires six biological components.
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# Red photosensor
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# Blue photosensor
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# AND gate
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# GFP fluorescent reporter
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# Promotable surface expression of cadherins
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# Gas vesicle expression to produce neutrally bouyant bacteria
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'''''High Level System Design'''''
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* Induce or constitutively express gas vesicles for neutral bouyancy
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* If Red & Blue light detected, then promote adhesion molecules.
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'''''Achievements'''''
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* Produced bouyant bacteria biobrick.[[Melbourne/Lab GV Notebook|(Have a look)]]
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* Produced parts of other biological components required.
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[[melb:Future Prospects|Conclusions and Future Work]]
 
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<center><h2><font face="broadway,verdana">Lab Procedures, Equipement ,Safety, Location</font></h2></center>
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<center><h2><font face="broadway,verdana">Procedures & Practical Matters</font></h2></center>
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* [[melb:Primary Reagents & disposables|Primary Reagents & disposables]]
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* [[Melbourne/Primary Reagents & disposables|Primary Reagents & disposables]]
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* [[melb:Protocols for Secondary Reagents|Protocols for secondary Reagents]]
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* [[Melbourne/Protocols for Secondary Reagents|Protocols for secondary Reagents and waste disposal]]
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* [[melb:Protocols for Standard Methods|Protocols for standard methods]]
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* [[Melbourne/Protocols for Standard Methods|Protocols for standard methods]]
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* [[melb:equipement|Fixed Equipement & tools]]
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* [[Melbourne/equipement|Fixed Equipement & tools]]
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* [[melb:Experiment|Experiment report]]
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* [[Melbourne/Software|Software links]]
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* [[melb:Lab Notebook|Lab notebook/diary]]
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* [[Melbourne/Lab Notebook|Lab notebook/diary]]
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* [[melb:Parts used|Parts Used by Melbourne]]
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* [[Melbourne/Parts used|Parts Used by Melbourne]]
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* [[melb:meeting minutes|Meeting Minutes]]
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* [[Melbourne/Parts created|Parts Created by Melbourne]]
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* [[melb:promotional material|Promotional Material]]
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* [[Melbourne/meeting minutes|Meeting Minutes]]
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<center><h2><font face="broadway,verdana">Sponsors</font></h2></center>
<center><h2><font face="broadway,verdana">Sponsors</font></h2></center>
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<td valign="top" width="60%">
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* University of Melbourne
* University of Melbourne
* Faculty of Biomedical Engineering
* Faculty of Biomedical Engineering
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<td valign="top" width="40%">
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* Bio21 Institute
* Bio21 Institute
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* Vice Chancellor (Research) ’s Office  
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* Vice Chancellor (Research) Office  
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Latest revision as of 16:29, 26 October 2007

Melbourne banner.png

Melbourne-team6.jpg The team welcomes you to the Melbourne University IGEM2007 Wiki!


"Coliforming"

Project overview

Background & references

Original Plan

Melbourne-team1.jpg

Melbourne-team7.jpg

Melbourne-team5.jpg


Project This project aims to use light to form a solid fluorescent mass of E. coli where two light beams intersect in a suspension of cells. We've called our building system "Coliforming".


Motivation Possible applications in building complex scaffolds in tissue engineering.


Requirements The system requires six biological components.

  1. Red photosensor
  2. Blue photosensor
  3. AND gate
  4. GFP fluorescent reporter
  5. Promotable surface expression of cadherins
  6. Gas vesicle expression to produce neutrally bouyant bacteria


High Level System Design

  • Induce or constitutively express gas vesicles for neutral bouyancy
  • If Red & Blue light detected, then promote adhesion molecules.


Achievements

  • Produced bouyant bacteria biobrick.(Have a look)
  • Produced parts of other biological components required.


Procedures & Practical Matters



Sponsors

  • University of Melbourne
  • Faculty of Biomedical Engineering
  • Bio21 Institute
  • Vice Chancellor (Research) Office


Retrieved from "http://2007.igem.org/wiki/index.php/Melbourne"