Berkeley LBL/Methods

From 2007.igem.org

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==Experimental==
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==Protocols==
[[Berkeley_LBL/PCRphusion|PCR (Using Phusion Polymerase)]]
[[Berkeley_LBL/PCRphusion|PCR (Using Phusion Polymerase)]]

Revision as of 20:05, 26 October 2007

Home Project Description Methods Notebook Results and Discussion Resources

Experimental

Protocols

PCR (Using Phusion Polymerase)

PCR (Using TaKaRa Ex Taq Polymerase)

PCR Clean Up/Purification

DNA Gel Electrophoresis

Miniprep

Digestion for PCR Product or Miniprepped DNA

Analytic Digestion

Gel Extraction

Ligation

KCM Competent Cell Production

KCM Competent Cell Transformation

Electroporation Transformation

Overexpression

Sonication

SDS-PAGE