PennState/Project/Diauxie/PromoterTest
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| - | <html><img src="http://openwetware.org/images/8/88/Pctest.jpg" id="logo" name="logo" height="150" width="300" alt="" /></html>This construct uses the xylF and xylA facing promoters that includes the CRP binding region. | + | <html><img src="http://openwetware.org/images/8/88/Pctest.jpg" id="logo" name="logo" height="150" width="300" alt="" /></html>This construct uses the xylF and xylA facing promoters that includes the CRP-cAMP binding region. |
| - | <html><img src="http://openwetware.org/images/d/d1/Pnctest.jpg" id="logo" name="logo" height="150" width="300" alt="" /></html>This construct uses the xylA facing | + | <html><img src="http://openwetware.org/images/d/d1/Pnctest.jpg" id="logo" name="logo" height="150" width="300" alt="" /></html>This construct uses the xylF and xylA facing promoters that do not include the CRP-cAMP binding region. |
| - | After each of the four promoter constructs are tested individually, the two | + | After each of the four promoter constructs are tested individually, the two which include the CRP-cAMP are changed so that a gene expressing the mutant CRP* is included. |
| + | |||
| + | <html><img src="http://openwetware.org/images/d/d1/Pnctest.jpg" id="logo" name="logo" height="150" width="300" alt="" /></html>This construct uses the xylF and xylA facing promoters that do not include the CRP-cAMP binding region followed by the CRP* gene. | ||
Revision as of 21:36, 26 October 2007
Promoter Test Constructs
To test each of the four xyl promoters, each is placed in a construct followed by a ribosome binding site, the reporter green florescent protein, and a terminator. The strength of each is measured by gfp emission.
This construct uses the xylF and xylA facing promoters that includes the CRP-cAMP binding region.
This construct uses the xylF and xylA facing promoters that do not include the CRP-cAMP binding region.
After each of the four promoter constructs are tested individually, the two which include the CRP-cAMP are changed so that a gene expressing the mutant CRP* is included.
This construct uses the xylF and xylA facing promoters that do not include the CRP-cAMP binding region followed by the CRP* gene.
