From 2007.igem.org

Revision as of 21:53, 26 October 2007 (view source) Jmonk (Talk | contribs) (→Experimentation) ← Older edit		Revision as of 21:54, 26 October 2007 (view source) Jmonk (Talk | contribs) (→Protocols) Newer edit →
Line 6:		Line 6:
	Please consult the relevant vendors for protocol documents.		Please consult the relevant vendors for protocol documents.
-	Assembly Line Flow	+	==Assembly Line Flow==
	Design Primers		Design Primers

---

Revision as of 21:54, 26 October 2007

Experimentation

Protocols

For reliability, a majority of our actual experimentation has followed protocols provided by the supplier of relevant projects, including enzymes ([http://www.neb.com/ NEB]) and kits ([http://www.qiagen.com/ Qiagen]). The [http://www.qiagen.com/ Qiagen] maxiprep kit was replaced with a [http://www.bio-rad.com/ Bio-Rad] kit to obtain higher yields with lower processing times.

Please consult the relevant vendors for protocol documents.

Assembly Line Flow

Design Primers

PCR

Run Gel

Gel Extraction and determine concentrations

Digestion with Restriction Enzymes

PCR purification and determine concentrations

Ligation