Princeton/lab/experimentation
From 2007.igem.org
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Revision as of 21:55, 26 October 2007
Contents |
Experimentation
Protocols
For reliability, a majority of our actual experimentation has followed protocols provided by the supplier of relevant projects, including enzymes ([http://www.neb.com/ NEB]) and kits ([http://www.qiagen.com/ Qiagen]). The [http://www.qiagen.com/ Qiagen] maxiprep kit was replaced with a [http://www.bio-rad.com/ Bio-Rad] kit to obtain higher yields with lower processing times.
Please consult the relevant vendors for protocol documents.
Assembly Line Flow
Bio-Brick Creation
Design Primers
PCR
Run Gel
Gel Extraction and determine concentrations
Digestion with Restriction Enzymes
PCR purification and determine concentrations
Ligation
