Ljubljana/biobricks

From 2007.igem.org

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Revision as of 22:18, 26 October 2007

Company Name

Biobricks

This year, our team has been the driving force of expanding the registry, creating no less than 77 parts. For our purpose we created, in addition to other parts, biobricks coding for individual domains of multi-domain proteins, involved in cellular signalling and antiviral response. These provide particular enrichment of the registry as they allow endless possibility to readily create novel chimeric proteins.

Slovenian Team Jewelbox

Following parts were deposited into BioBrick database:


NameTypeDescriptionLength
  BBa_I712002CodingCCR51059
  BBa_I712003CodingCCR5-NUb1194
   BBa_I712004RegulatoryCMV promoter654
  BBa_I712007Tagc-Myc-tag30
  BBa_I712008TagHA-tag30
  BBa_I712009CodingCD4 signal peptide75
  BBa_I712010CodingCD4 sequence without signal peptide1299
  BBa_I712011CodingCUb (split ubiquitin system)135
  BBa_I712012CodingTIR domain of TLR3456
  BBa_I712015Othercleavage site for HIV-1 protease42
  BBa_I712016Othermyri + TEVP57
  BBa_I712017CodingChemokine (CXC motif) receptor 4, fused to N-terminal half of ubiquitin.1191
  BBa_I712019ReporterFirefly luciferase - luciferase from Photinus pyralis1653
  BBa_I712020CodingHomo sapiens caspase 3834
  BBa_I712021CodingInterferon beta564
  BBa_I712022CodingT7 RNA polymerase with NLS2673
  BBa_I712024CodingCD41374
  BBa_I712026Tagmyristoilation site21
  BBa_I712027CompositeCMV + CCR5-NUb1854
  BBa_I712028CodingCherryNLS - synthetic construct monomeric red fluorescent protein with nuclear localization sequence733
  BBa_I712029CompositeCMV-myc-CXCR4-NUb1887
  BBa_I712030CompositeCMV-CXCR4-NUb1851
  BBa_I712031IntermediateCMVp-CD4-HA-CUb2219
  BBa_I712033IntermediateCMVp - myristoilation site681
  BBa_I712034IntermediateCMVp - myristoilation site - HIV protease cleavage site731
  BBa_I712035CompositeCMVp-CD4-CUb2175
  BBa_I712037CompositeCMVp - CD4 - CUb - mCherry - NLS2914
  BBa_I712038CompositeCMVp - CD4 - CUb - GFP2901
  BBa_I712039IntermediateCMVp - HA - CD4 - CUb - GFP2945
  BBa_I712040IntermediateCMVp-CD4-HIVcleavageSite2084
  BBa_I712041CompositeCMVp - CD4 - HIV protease cleavage site - mCherryNLS2823
  BBa_I712043CompositeCMVp - myristoilation site - HIV protease cleavage site - T7RNAP - NLS3410
  BBa_I712044CompositeCMVp - myristoilation site - HIV protease cleavage site - mCherry - NLS1470
  BBa_I712045CompositeCMVp - myristoilation site - HIV protease cleavage site - GFP1457
  BBa_I712046CompositeCMVp - HA - CD4 - HIV protease cleavage site - GFP2854
  BBa_I712048CompositeCMV + CD4 with ha tag + TIR (TLR3)2542
   BBa_I712049CompositeCMV + Myc + CCR5 + TIR(TLR3)2183
  BBa_I712050CompositeCMV promoter - RBS - T7 RNA polymerase - NLS3340
  BBa_I712054ReporterpT7-mCer772
  BBa_I712055Compositemyri - TEVP - mCherry - NLS796
  BBa_I712056Compositemyri - TEVP - GFP783
  BBa_I712057Compositemyri - TEVP - T7 RNAP - NLS2736
  BBa_I712058CompositeCMVp - myri - TEVP - GFP1443
  BBa_I712059CompositeCMVp - myri - TEVP - T7 RNAP - NLS3396
  BBa_I712064CompositepT7-IFNbeta616
  BBa_I712069CodingRBS-T7 RNA polymerase with NLS2678
  BBa_I712070CompositeT7 promoter - RBS -T7 RNA polymerase with NLS2732
  BBa_I712073DeviceCMV-Cas31494
  BBa_I712074RegulatoryT7 promoter (strong promoter from T7 bacteriophage)46
   BBa_I712076CodingCMV promoter - Myc tag - CCR5 (shortened)1608
  BBa_I712077CodingTEV protease (N-terminal half)357
  BBa_I712078CodingTEV protease (C-terminal half)375
  BBa_I712079CodingCMV promoter - Myc tag - CCR5 (shortened) - TEV N1973
  BBa_I712080CodingCMV promoter - Myc tag - CCR5 (shortened) - TLR3 TIR2072
  BBa_I712082CodingCMV promoter - CD4 (shortened)1907
  BBa_I712083CompositeCMV promoter - CD4 (shortened) - TEV C2290
  BBa_I712084CompositeCMV promoter - CD4 (shortened) - TLR3 TIR2371
  BBa_I712085CompositeCMV - CD4(shortened) - Cub2048
  BBa_I712086CompositeCMV - CD4(shortened) - Cub - CherryNLS2646
  BBa_I712087CompositeCMV - CD4(shortened) - Cub - T7polNLS4727
  BBa_I712088CompositeCMV - CD4(shortened) - Cub - GFP2774
  BBa_I712090CompositeCMVp-CD42034
  BBa_I712564CompositeCMV + fLuc2313
  BBa_I712666CompositeCMV + Myc + CCR51755
   BBa_I712667CodingHIV protease303
   BBa_I712668CompositeCMV - HIV protease963
   BBa_I712669CompositeCMV - mCer1380
  BBa_I712670IntermediateCMV-HA690
  BBa_I712671IntermediateCMV-c-myc690
  BBa_I712672CompositeCMV-IFNbeta1224