Paris/October 19
From 2007.igem.org
< Paris(Difference between revisions)
Eismoustique (Talk | contribs) |
(→Digestion reactions) |
||
| (8 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| + | [[Paris/October 18|yesterday]] -- [[Paris/October 20|tomorrow]] <br> | ||
| + | == FACS == | ||
| + | |||
| + | == PCR reactions == | ||
| + | |||
| + | |||
| + | |||
== Digestion reactions == | == Digestion reactions == | ||
| Line 16: | Line 23: | ||
|width=1%| | |width=1%| | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" |D22bis |
| - | | | + | |pSB1A2 BBv |
| - | | | + | |MP6.1 et .2 |
|EcoRI | |EcoRI | ||
|PstI | |PstI | ||
| | | | ||
| - | | | + | |pSB1A2 vector digested with EcoRI and PstI enzymes |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| | | | ||
| | | | ||
| Line 56: | Line 52: | ||
| | | | ||
|pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning | |pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| | | | ||
| | | | ||
| Line 128: | Line 58: | ||
| style="background: #ccffcc;" |D88 | | style="background: #ccffcc;" |D88 | ||
|pTet>>lox71-gfptripqrt FV | |pTet>>lox71-gfptripqrt FV | ||
| - | |L34 | + | |L34.1 et .2 |
|EcoRI | |EcoRI | ||
|XbaI | |XbaI | ||
| Line 139: | Line 69: | ||
| style="background: #ccffcc;" |D89 | | style="background: #ccffcc;" |D89 | ||
|T-lox66-rbsdapAcoli BV | |T-lox66-rbsdapAcoli BV | ||
| - | |L53 | + | |L53.7 |
|SpeI | |SpeI | ||
|PstI | |PstI | ||
| Line 161: | Line 91: | ||
| style="background: #ccffcc;" |D91 | | style="background: #ccffcc;" |D91 | ||
|ptet>>lox71-gfpT-lox66-mrfp pSB1A2 BV | |ptet>>lox71-gfpT-lox66-mrfp pSB1A2 BV | ||
| - | |L59 | + | |L59.6, .7 |
|SpeI | |SpeI | ||
|PstI | |PstI | ||
| Line 170: | Line 100: | ||
| | | | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" |D92 |
|rbsdapAcoli BI | |rbsdapAcoli BI | ||
| - | |L39 | + | |L39.1 |
|XbaI | |XbaI | ||
|PstI | |PstI | ||
| Line 181: | Line 111: | ||
| | | | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" |D93 |
|rbsdapAsubtilis BI | |rbsdapAsubtilis BI | ||
| - | |L64 | + | |L64.1, .2 |
|XbaI | |XbaI | ||
|PstI | |PstI | ||
Latest revision as of 23:38, 26 October 2007
FACS
PCR reactions
Digestion reactions
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description | |||
| D22bis | pSB1A2 BBv | MP6.1 et .2 | EcoRI | PstI | pSB1A2 vector digested with EcoRI and PstI enzymes | ||||
| D80 | PacI[ptet>>lox71-gfpT-lox66-mrfp]SmaI | P15 | PacI | SmaI | ptet>>lox71-gfpT-lox66-mrfp P15 PCR product digested by PacI-SmaI for cloning into pY-2C-IntC | ||||
| D81 | PacI[pY-2C-IntC]SmaI | pY-2C-IntC plasmid | PacI | SmaI | pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning | ||||
| D88 | pTet>>lox71-gfptripqrt FV | L34.1 et .2 | EcoRI | XbaI | pTet>>lox71-gfptripqrt pSB1A2 dig. for use as a FV | ||||
| D89 | T-lox66-rbsdapAcoli BV | L53.7 | SpeI | PstI | T-lox66-rbsdapAcoli pSB1A2 dig. for use as a BV | ||||
| D90 | T-lox66-rbsdapAsubtilis BV | L56 | SpeI | PstI | T-lox66-rbsdapAsubtilis pSB1A2 dig. for use as a BV | ||||
| D91 | ptet>>lox71-gfpT-lox66-mrfp pSB1A2 BV | L59.6, .7 | SpeI | PstI | ptet>>lox71-gfpT-lox66-mrfp pSB1A2 dig. for use as a BV | ||||
| D92 | rbsdapAcoli BI | L39.1 | XbaI | PstI | rbsdapAcoli extracted for use as a BI | ||||
| D93 | rbsdapAsubtilis BI | L64.1, .2 | XbaI | PstI | rbsdapAsubtilis extracted for use as a BI | ||||
