Imperial/Cell by Date/Implementation

From 2007.igem.org

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|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
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* '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/CE1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Chas1.1 |Results]])
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* '''Testing ''in vitro''''': Failed ([[Imperial/Wet_Lab/Protocols/CE1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Chas1.1 |Results]])
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* '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/CBD1.2|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/CBD1.2 Results])
* '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/CBD1.2|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/CBD1.2 Results])

Revision as of 01:41, 27 October 2007


Cell by Date: Implementation

DNA Construct Implementation
Construct Design Concept 1
IC07 design CBDcon1.png
 Concept 2
IC07 design CBDcon2.png
Stage 1: Availability

Icegems-Devcycle-downarrow.png 		Found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in Registry Found as [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] in Registry.
Stage 2: Cloning / Amplification

Icegems-Devcycle-downarrow.png
BBa_I13522 - Lane R2 (937bp + 2079bp vector)
BBa_E7104 - Lane 11 (Missing 826bp)
Stage 3: Ligation

Icegems-Devcycle-downarrow.png 		Not Required Since [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] was not functional, we made our own promoter and and ligated it to [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]


Imperial CBD Design CBD4.png + Imperial CBD Design CBD3.png = Imperial CBD Design CBD2.2.png
Stage 4: Purification of Activator Proteins

Icegems-Devcycle-downarrow.png 		Not Required Not Required
Stage 5: Quality Control

Icegems-Devcycle-downarrow.png
IC2007 Experiments Phase1 Protocol1-1.jpg

  • Testing in vivo: Passed (Protocols and Results)
  • Sequencing of Construct: Completed
IC 2007 PT7-invitro.png

  • Testing in vitro: Passed (Protocols and Results)
  • Sequencing of Construct: Completed
Stage 6: Final Implementation Status Ready for Testing Ready for Testing



Chassis Construct Implementation
Chassis Design IC07 CFS components.png
Stage 1: Availability

Icegems-Devcycle-downarrow.png 		Possibility of making Homemade S30 Cell Extract. Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega].
Stage 2: Preparation

Icegems-Devcycle-downarrow.png 		Preparation: Done (Protocol) Preparation: Done (Protocol)
Stage 3: Quality Control

Icegems-Devcycle-downarrow.png
Stage 4: Final Implementation Status Aborted.
Expression capacity of chassis was low. 		Ready for Testing



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