PennState/Project/Dosimeter/FinalConstructs
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| + | '''Final Parts''' | ||
| + | Once the dose threshold is estabilished using the test construct, the lambda promoter with corrected cro RBS is placed upstream of readily identifiable reporter proteins for an easy to use warning system. Luciferase and mCherry expressed simultaneously would provide good signal visibility in a range of lighting conditions. | ||
| + | <html><img src="http://openwetware.org/images/thumb/e/e8/PSU_IGEM_GFL_mCherryDosimeter.jpg/800px-PSU_IGEM_GFL_mCherryDosimeter.jpg" id="logo" name="logo" height="128" alt="" /><br></html> | ||
| - | + | This construct is the final dosimeter construct that produces mCherry and luciferase in response to a threshold dose of radiation. | |
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<html> | <html> | ||
<!-- START OF LUC'S CODE --> | <!-- START OF LUC'S CODE --> | ||
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<div class="podcast"> | <div class="podcast"> | ||
<!-- this is where you need to post the link to where it will go (the second one)--> | <!-- this is where you need to post the link to where it will go (the second one)--> | ||
| - | <h3><a rel="enclosure" href="https://2007.igem.org/PennState/Project/ | + | <h3><a rel="enclosure" href="https://2007.igem.org/PennState/Project/Dosimeter" ="window.popup_player_258600 = window.open('https://2007.igem.org/PennState/Project/Dosimeter','post_258600','toolbar=no,scrollbars=no,directories=no,resizable=yes,width=360,height=305,top=20,left=20,location=no,menubar=no,status=yes,'); return false;"> |
Introduction | Introduction | ||
</a></h3> | </a></h3> | ||
<div class="screenshot"> | <div class="screenshot"> | ||
| - | <!-- little white box thing --> <a rel="enclosure" href="https://2007.igem.org/PennState/Project/ | + | <!-- little white box thing --> <a rel="enclosure" href="https://2007.igem.org/PennState/Project/Dosimeter" |
<img src="http://openwetware.org/images/9/97/Psuigem2007promoterimage.jpg" width="133" height="100" alt="" /> | <img src="http://openwetware.org/images/9/97/Psuigem2007promoterimage.jpg" width="133" height="100" alt="" /> | ||
</a> | </a> | ||
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<div class="podcast"> | <div class="podcast"> | ||
<!-- this is where you need to post the link to where it will go (the second one)--> | <!-- this is where you need to post the link to where it will go (the second one)--> | ||
| - | <h3><a rel="enclosure" href="https://2007.igem.org/PennState/Project/ | + | <h3><a rel="enclosure" href="https://2007.igem.org/PennState/Project/Dosimeter/InitialTest" ="window.popup_player_258600 = window.open('https://2007.igem.org/PennState/Project/Dosimeter/InitialTest','post_258600','toolbar=no,scrollbars=no,directories=no,resizable=yes,width=360,height=305,top=20,left=20,location=no,menubar=no,status=yes,'); return false;"> |
| - | + | Test Constructs | |
</a></h3> | </a></h3> | ||
<div class="screenshot"> | <div class="screenshot"> | ||
| - | <!-- little white box thing --> <a rel="enclosure" href="https://2007.igem.org/PennState/Project/ | + | <!-- little white box thing --> <a rel="enclosure" href="https://2007.igem.org/PennState/Project/Dosimeter/InitialTest" |
<img src="http://openwetware.org/images/b/be/Psuigem2007RBSimage.jpg" width="133" height="100" alt="" /> | <img src="http://openwetware.org/images/b/be/Psuigem2007RBSimage.jpg" width="133" height="100" alt="" /> | ||
</a> | </a> | ||
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<div class="column"><div class="podcast"> | <div class="column"><div class="podcast"> | ||
<!-- this is where you need to post the link to where it will go (the second one)--> | <!-- this is where you need to post the link to where it will go (the second one)--> | ||
| - | <h3><a rel="enclosure" href="https://2007.igem.org/PennState/Project/ | + | <h3><a rel="enclosure" href="https://2007.igem.org/PennState/Project/Dosimeter/Dosimetry" ="window.popup_player_258600 = window.open('http://openwetware.org/images/c/c9/XylRdimer1.png','post_258600','toolbar=no,scrollbars=no,directories=no,resizable=yes,width=360,height=305,top=20,left=20,location=no,menubar=no,status=yes,'); return false;"> |
| - | + | Dosimetry | |
</a></h3> | </a></h3> | ||
<div class="screenshot"> | <div class="screenshot"> | ||
| - | <!-- little white box thing --> <a rel="enclosure" href="https://2007.igem.org/PennState/Project/ | + | <!-- little white box thing --> <a rel="enclosure" href="https://2007.igem.org/PennState/Project/Dosimeter/Dosimetry" |
<img src="http://openwetware.org/images/2/21/Psuigem2007geneimage.jpg" width="133" height="100" alt="" /> | <img src="http://openwetware.org/images/2/21/Psuigem2007geneimage.jpg" width="133" height="100" alt="" /> | ||
</a> | </a> | ||
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<div class="column"><div class="podcast"> | <div class="column"><div class="podcast"> | ||
<!-- this is where you need to post the link to where it will go (the second one)--> | <!-- this is where you need to post the link to where it will go (the second one)--> | ||
| - | <h3><a rel="enclosure" href="https://2007.igem.org/PennState/Project/ | + | <h3><a rel="enclosure" href="https://2007.igem.org/PennState/Project/Dosimeter/FinalConstructs" ="window.popup_player_258600 = window.open('https://2007.igem.org/PennState/Project/Dosimeter/FinalConstructs','post_258600','toolbar=no,scrollbars=no,directories=no,resizable=yes,width=360,height=305,top=20,left=20,location=no,menubar=no,status=yes,'); return false;"> |
Final Constructs | Final Constructs | ||
</a></h3> | </a></h3> | ||
<div class="screenshot"> | <div class="screenshot"> | ||
| - | <!-- little white box thing --> <a rel="enclosure" href="https://2007.igem.org/PennState/Project/ | + | <!-- little white box thing --> <a rel="enclosure" href="https://2007.igem.org/PennState/Project/Dosimeter/FinalConstructs" |
<img src="http://openwetware.org/images/9/9b/Psuigem2007TTimage.jpg" width="133" height="100" alt="" /> | <img src="http://openwetware.org/images/9/9b/Psuigem2007TTimage.jpg" width="133" height="100" alt="" /> | ||
</a> | </a> | ||
Latest revision as of 03:29, 27 October 2007
Final Parts
Once the dose threshold is estabilished using the test construct, the lambda promoter with corrected cro RBS is placed upstream of readily identifiable reporter proteins for an easy to use warning system. Luciferase and mCherry expressed simultaneously would provide good signal visibility in a range of lighting conditions.
This construct is the final dosimeter construct that produces mCherry and luciferase in response to a threshold dose of radiation.
